Bacterial diversity and functional characteristics of natural rainwater lakes in Saihanba artificial forest farm as revealed by 16S rRNA sequencing

The Saihanba Mechanical Forest is an artificial national forest park with a forest-steppe landscape. The flora and fauna have been extensively studied, but a comprehensive understanding of the bacterial community composition and structure of the natural rainwater lakes present in the area has rarely been reported. In this study, the structure and functional characteristics of bacterial communities in lake sediments and water samples in the Saihanba artificial forest were investigated using 16s rRNA high-throughput sequencing. Microbial diversity analyses revealed that Proteobacteria, Acidobacteria, Bacteroidota and Verrucomicrobiota microbiota dominated. The abundance of Proteobacteria and Actinobacteriota was significantly higher (p < .05) in water samples compared to sediment samples. PICRUSt2 functional analysis predicted genes associated with the degradation of xenobiotics and the execution of essential metabolic processes. Here, we report differences in the composition of native bacterial communities in sediments and water under the Saihanba artificial forest and make functional gene predictions. This study provides a reference for further exploring the structure and functional characteristics of microbial communities in water samples and sediment environments of lakes under planted forests.</p

Mental ion effect on <i>At</i>GUS-E and <i>At</i>GUS(-3t)-E.

<p>Mental ion effect on <i>At</i>GUS-E and <i>At</i>GUS(-3t)-E.</p

Far-UV spectra of <i>At</i>GUS-E and <i>At</i>GUS(-3t)-E.

<p>Far-UV spectra were recorded at 25°C in the range from 200 to 250 nm with a spectral resolution of 0.2 nm.</p

Intron deletion of <i>At</i>gus (A) and electrophoresis of the target sequence(B and C).

<p>M: marker, DL2000; L1: the target gene with intron; L2: the full length of the gene without intron(<i>At</i>gus); L3: the target gene without C-terminal non-conservative sequence[<i>At</i>gus(-3t)].</p

Enzymatic properties of <i>At</i>GUS-E and <i>At</i>GUS(-3t)-E.

<p>(A) the optimum pH, (B) the optimum temperature, and the thermal stability of <i>At</i>GUS(-3t)-E (C) and <i>At</i>GUS-E (D).</p

Domains analysis of <i>At</i>GUS, <i>P</i>GUS and <i>Au</i>GUS.

a<p>the E value was obtained by alignment with pfam02837, glycosyl hydrolase family 2, sugar binding domain;</p>b<p>the E value was obtained by alignment with pfam00703, glycosyl hydrolase family 2, immunoglobulin-like beta-sandwich domain;</p>c<p>the E value was obtained by alignment with pfam02836, glycosyl hydrolase family 2, TIM barrel domain.</p

A three-dimensional model of β-glucuronidase(<i>At</i>GUS).

<p>I: sugar binding domain; II: immunoglobulin-like beta-sandwich domain; III: TIM barrel domain; IV: non-conservative domain; catalytic face and stability face were shown CF and SF for short.</p

Additional file 1: of Effect of in ovo folic acid injection on hepatic IGF2 expression and embryo growth of broilers

The summary of regression analysis for methylation data. (DOC 42 kb

Genotype of rs11077 SNP site in XPO5 and its association with HCC survival.

<p>rs11077 SNP site in XPO5 (C/C: SNPs, A/C: heterozygous SNP, A/A:WT). Cum =  cumulative.</p

Phylogenetic tree of target protein with GHF2 β-glucuronidase (GUS), β-galactosidase (GAL), β-mannosidase (MAN).

<p>The scale corresponds to a genetic distance of 0.2 substitution per position (20% difference). <i>Aspergillus niger</i> CBS 513.88 GUS: XP_001388566; <i>Penicillium purpurogenum</i> GUS: ABU68712; <i>Aspergillus oryzae</i> RIB40 GUS: XP_001825002; <i>Penicillium canescens</i> GUS: AAV91787; <i>Escherichia coli</i> K-12 GUS: AAC74689; <i>Mus musculus</i> GUS: AAA37696; <i>Chlorocebus aethiops</i> GUS: AAC34593; <i>Canis lupus</i> GUS: AAC48809; <i>Thermotoga maritima</i> MSB8 GUS: AAD36143; <i>Kluyveromyces lactis</i> GAL: AAA35265; <i>Bacillus megaterium</i> DSM 319 GAL: CAA04267; <i>Lactobacillus sakei</i> GAL: CAA57730; <i>Pseudoalteromonas haloplanktis</i> GAL: CAA10470; <i>Escherichia coli</i> GAL: AAA24053; <i>Cellulomonas fimi</i> ATCC 484 MAN: AAD42775; <i>Aspergillus aculeatus</i> MAN: BAA29029; <i>Mus musculus</i> MAN: AAK18177; <i>Bos Taurus</i> MAN: AAC48460; <i>Homo sapiens</i> MAN: AAC39573.</p