Table1_Modification of m5C regulators in sarcoma can guide different immune infiltrations as well as immunotherapy.xls

BackgroundRecent studies have found that 5-methylcytosine (m5C) modulators are associated with the prognosis and treatment of cancer. However, the relevance of m5C modulators in sarcoma prognosis and the tumour microenvironment is unclear.MethodsWe selected 15 m5C regulators and performed unsupervised clustering to identify m5C modification patterns and differentially expressed genes associated with the m5C phenotype in The Cancer Genome Atlas (TCGA) sarcomas. The extent of immune cell infiltration in different clustering groups was explored using single-sample gene set enrichment analysis and estimation algorithms. A principal component analysis algorithm-based m5C scoring protocol was performed to assess the m5C modification patterns of individual tumors.ResultsWe identified two distinct m5C modification patterns in the TCGA sarcoma cohort, which possess different clinical outcomes and biological processes. Tumour microenvironment analysis revealed two groups of immune infiltration patterns highly consistent with m5C modification patterns, classified as immune inflammatory and immune desert types. We constructed m5C scores and found that high m5C scores were closely associated with leiomyosarcoma and other subtypes, and were associated with poorer prognosis, lower PD-L1 expression, and poorer immunotherapy outcomes. The best application was validated against the m5C database.ConclusionWe constructed an m5C score for sarcoma based on the TCGA database and identified a poorer prognosis in the high m5c score group. The stability and good prognostic predictive power of the m5C score was verified by an external database. We found that sarcomas in the low m5C score group may have a better response to immunotherapy.</p

New dammarane-type triterpenoids from the whole plant of <i>Euphorbia hypericifolia</i>

Euphorhypenoids A (1) and B (2), two new dammarane-type triterpenoids, along with four known tetracyclic triterpenoids (3−6), were isolated from the whole plant of Euphorbia hypericifolia. The structures of new compounds were mainly elucidated by a series of extensive spectroscopic methods, including HR-ESI-MS, NMR, IR, and UV. Compound 1 exhibited significant inhibitory effect on platelet aggregation at concentrations of 10 − 200 µM.</p

Amorphous Cobalt Phosphate Porous Braced-Frame Nanocubes as Highly Efficient Supercapacitor Electrodes

It is extremely urgent to develop novel electrode materials with large capacity, high charge–discharge speed, and cycle stability for the particular application of supercapacitors in electric vehicles. Cobalt-based phosphate materials have attracted increasing attention due to their outstanding properties. Herein, cobalt phosphate with a braced-frame nanocube morphology consisting of amorphous nanoparticles is synthesized through a one-step ion-exchange method using Prussian Blue Analog Co3[Co(CN)6]2 nanocubes as precursors. Benefiting from the special nanostructure, this material shows a specific capacitance of up to 539.2 F g–1 at 1 A g–1, a capacitance retention of 92% even at 8 A g–1, and remarkable cycle stability characteristics, with a capacity preservation of 89.1% after 3000 charge–discharge cycles at 2 A g–1. The kinetic mechanism demonstrates that the diffusion-controlled procedure has made a great contribution to the large capacitance. This study will be helpful for the development of new electrode materials for supercapacitors