Direct Extrusion Freeforming of Ceramic Pastes

Microextrusion freeforming of ceramic lattices from high solids ceramic pastes provides multi-scale hierarchical void structures with the advantages of low shrinkage stress and high sintered density. Alumina lattices were directly fabricated using 80-500 Pm diameter filaments. We report here on the implementation of design and fabrication of these scaffolds for band gap materials and micro fluidic devices.Mechanical Engineerin

Computer Generation of Metal Components by Simultaneous Deposition of Mould, Cores and Part

A new solid freeforming method based on co-delivery of mould powder materials and part powder materials using vibration-controlled, dry powder valves is presented in this paper. Thin layers of stainless steel powder are delivered to the forming area according to the cross-section of the CAD file to produce the component. Mould powder which has low sinterability is delivered to the non-forming areas of the same layer. All powders are delivered by computer-controlled, acoustic powder valves. The flow rate and switching of the valves provides the composition and shape control during fabrication. The stacked layers of loose powder are then sintered in a conventional furnace. The mould materials are removed after sintering. This method avoids the high thermal stress problem in selective laser sintering, avoids high capitalisation, makes use of conventional furnaces and allows for the incorporation of three dimensional function gradients. Test pieces including step wedge and Spierpinski’s cube were fabricated. Advantages, limitations and problems are discussed.Mechanical Engineerin

A novel bioactive PEEK/HA composite with controlled 3D interconnected HA network

Polyetheretherketone (PEEK) is a high-performance thermoplastic biomaterial which is currently used in a variety of biomedical orthopaedic applications. It has comparable tensile and compressive strength to cortical bone with favourable biocompatibility. However, natural grade PEEK-OPTIMA has shown insufficient bioactivity and limited bone integration. Bioactive PEEK composites (e.g., PEEK/calcium phosphates or Bioglass) and porous PEEK have been used to improve bone-implant interface of PEEK-based devices, but the bioactive phase distribution or porosity control is poor. In this paper, a novel method is developed to fabricate a bioactive PEEK/hydroxyapatite (PEEK/HA) composite with a unique configuration in which the HA (bioactive phase) distribution is computer-controlled within a PEEK matrix. This novel process results in complete interconnectivity of the HA network within a composite material, representing a superior advantage over alternative forms of product. The technique combines extrusion freeforming, a type of additive manufacturing (AM), and compression moulding. Compression moulding parameters, including pressure, temperature, dwelling time, and loading method together with HA microstructure were optimized by experimentation for successful biocomposite production. PEEK/HA composites with a range of HA were produced using static pressure loading to minimise air entrapment within PEEK matrix. In addition, the technique can also be employed to produce porous PEEK structures with controlled pore size and distribution

Dry Powder Microfeeding System for Solid Freeform Fabrication

Second generation SFF techniques allow both composition and shape to be downloaded directly from a computer file so that 3D functionally graded materials (FGM) can be assembled. Methods for multi-material deposition are also needed in combinatorial research, colour management and pharmaceutical dosing. In this work, computer-controlled microfeeding systems using ultrasonic vibration of a capillary were built. A wide range of stable flow rate control and switching control were achieved in the acoustic vibration system, and uniform powder doses were obtained in the ultrasonic system. The experimental results show that the nozzle diameter, transmission fluid depth, waveforms, voltage amplitude, frequency and oscillation duration all influence the dose mass. Among these factors, the nozzle diameter, voltage amplitude and oscillation duration can be used to control the dose mass. Raster printing of patterns with various resolution and dot size are demonstrated.Mechanical Engineerin

Generic protease detection technology for monitoring periodontal disease

Periodontal diseases are inflammatory conditions that affect the supporting tissues of teeth and can lead to destruction of the bone support and ultimately tooth loss if untreated. Progression of periodontitis is usually site specific but not uniform, and currently there are no accurate clinical methods for distinguishing sites where there is active disease progression from sites that are quiescent. Consequently, unnecessary and costly treatment of periodontal sites that are not progressing may occur. Three proteases have been identified as suitable markers for distinguishing sites with active disease progression and quiescent sites: human neutrophil elastase, cathepsin G and MMP8. Generic sensor materials for the detection of these three proteases have been developed based on thin dextran hydrogel films cross-linked with peptides. Degradation of the hydrogel films was monitored using impedance measurements. The target proteases were detected in the clinically relevant range within a time frame of 3 min. Good specificity for different proteases was achieved by choosing appropriate peptide cross-linkers.<br/

Agent-Based Modeling and Simulation for the Bus-Corridor Problem in a Many-to-One Mass Transit System

With the growing problem of urban traffic congestion, departure time choice is becoming a more important factor to commuters. By using multiagent modeling and the Bush-Mosteller reinforcement learning model, we simulated the day-to-day evolution of commuters’ departure time choice on a many-to-one mass transit system during the morning peak period. To start with, we verified the model by comparison with traditional analytical methods. Then the formation process of departure time equilibrium is investigated additionally. Seeing the validity of the model, some initial assumptions were relaxed and two groups of experiments were carried out considering commuters’ heterogeneity and memory limitations. The results showed that heterogeneous commuters’ departure time distribution is broader and has a lower peak at equilibrium and different people behave in different pattern. When each commuter has a limited memory, some fluctuations exist in the evolutionary dynamics of the system, and hence an ideal equilibrium can hardly be reached. This research is helpful in acquiring a better understanding of commuter’s departure time choice and commuting equilibrium of the peak period; the approach also provides an effective way to explore the formation and evolution of complicated traffic phenomena

The 3D printing of a polymeric electrochemical cell body and its characterisation

An undivided flow cell was designed and constructed using additive manufacturing technology and its mass transport characteristics were evaluated using the reduction of ferricyanide, hexacyanoferrate (III) ions at a nickel surface. The dimensionless mass transfer correlation Sh = aRebScdLee was obtained using the convective-diffusion limiting current observed in linear sweep voltammetry; this correlation compared closely with that reported in the literature from traditionally machined plane parallel rectangular flow channel reactors. The ability of 3D printer technology, aided by computational graphics, to rapidly and conveniently design, manufacture and re-design the geometrical characteristics of the flow cell ishighlighted

6G Network Operation Support System

6G is the next-generation intelligent and integrated digital information infrastructure, characterized by ubiquitous interconnection, native intelligence, multi-dimensional perception, global coverage, green and low-carbon, native network security, etc. 6G will realize the transition from serving people and people-things communication to supporting the efficient connection of intelligent agents, and comprehensively leading the digital, intelligent and green transformation of the economy and the society. As the core support system for mobile communication network, 6G OSS needs to achieve high-level network automation, intelligence and digital twinning capabilities to achieve end-to-end autonomous network operation and maintenance, support the operation of typical 6G business scenarios and play a greater social responsibility in the fields of environment, society, and governance (ESG).This paper provides a detailed introduction to the overall vision, potential key technologies, and functional architecture of 6G OSS . It also presents an evolutionary roadmap and technological prospects for the OSS from 5G to 6G.Comment: 103 pages, 20 figures, 52 references (chinese version

SY18ΔL60L: a new recombinant live attenuated African swine fever virus with protection against homologous challenge

IntroductionAfrican swine fever (ASF) is an acute and highly contagious disease and its pathogen, the African swine fever virus (ASFV), threatens the global pig industry. At present, management of ASF epidemic mainly relies on biological prevention and control methods. Moreover, due to the large genome of ASFV, only half of its genes have been characterized in terms of function.MethodsHere, we evaluated a previously uncharacterized viral gene, L60L. To assess the function of this gene, we constructed a deletion strain (SY18ΔL60L) by knocking out the L60L gene of the SY18 strain. To evaluate the growth characteristics and safety of the SY18ΔL60L, experiments were conducted on primary macrophages and pigs, respectively.ResultsThe results revealed that the growth trend of the recombinant strain was slower than that of the parent strain in vitro. Additionally, 3/5 (60%) pigs intramuscularly immunized with a 105 50% tissue culture infectious dose (TCID50) of SY18ΔL60L survived the 21-day observation period. The surviving pigs were able to protect against the homologous lethal strain SY18 and survive. Importantly, there were no obvious clinical symptoms or viremia.DiscussionThese results suggest that L60L could serve as a virulence- and replication-related gene. Moreover, the SY18ΔL60L strain represents a new recombinant live-attenuated ASFV that can be employed in the development of additional candidate vaccine strains and in the elucidation of the mechanisms associated with ASF infection

Generic protease detection technology for monitoring periodontal disease

Periodontal diseases are inflammatory conditions that affect the supporting tissues of teeth and can lead to destruction of the bone support and ultimately tooth loss if untreated. Progression of periodontitis is usually site specific but not uniform, and currently there are no accurate clinical methods for distinguishing sites where there is active disease progression from sites that are quiescent. Consequently, unnecessary and costly treatment of periodontal sites that are not progressing may occur. Three proteases have been identified as suitable markers for distinguishing sites with active disease progression and quiescent sites: human neutrophil elastase, cathepsin G and MMP8. Generic sensor materials for the detection of these three proteases have been developed based on thin dextran hydrogel films cross-linked with peptides. Degradation of the hydrogel films was monitored using impedance measurements. The target proteases were detected in the clinically relevant range within a time frame of 3 min. Good specificity for different proteases was achieved by choosing appropriate peptide cross-linkers.<br/