Identification of <i>B. mori</i> proteins separated by SDS-PAGE.

<p>Identification of <i>B. mori</i> proteins separated by SDS-PAGE.</p

Hypothesized roles of the identified BmNPV-binding proteins of <i>B. mori</i> in the virus infection process.

<p>The envelope contained BmNPV nucleocapsid binded to the cytomembrane, then endocytosis was triggered, and vacuolar ATP synthase catalytic subunit A and subunit B on the endosome membrane could promote the fusion of the envelope and endosome to release the nucleocapsid into the cytoplasm. The released nucleocapsid was transported into the nucleus with the assistance of cytoskeleton (Actin, PGK and En). The virus DNA was released in the nucleus, and RP0 could regulate RNA expression of BmNPV. In the infection process, GDAP would trigger apotosis to inhibit BmNPV infection, while PHB2, CRT, RCX1 and HSP60 had the opposite effects.</p

Identification of <i>B. mori</i> proteins separated by 2-DE.

<p>The bands and spots numbers corresponded to the numbers given in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0115032#pone-0115032-g001" target="_blank">Fig. 1</a>, <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0115032#pone-0115032-g002" target="_blank">Fig. 2</a> and <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0115032#pone-0115032-g003" target="_blank">Fig. 3</a>. Protein bands and spots were subjected to in-gel trypsin digestion. Protein fragments were then analyzed by MALDI-TOF/TOF MS analysis on ABI 4800 Plus MALDI TOF/TOF Analyzer. The peptide sequences obtained from MALDI-TOF/TOF MS were searched against the protein sequences from NCBInrmetazoa using the Mascot algorithm (<a href="http://www.matrixscience.com" target="_blank">http://www.matrixscience.com</a>). Protein identification was accepted when the matching scores were significant at P<0.05, as based on the Mowse score (Matrix Science, London, UK).</p><p>Identification of <i>B. mori</i> proteins separated by 2-DE.</p

Virus binding experiment on total proteins of <i>B. mori</i> midgut resolved by 2-DE.

<p>A) Virus overlay binding experiment. B) Separation of <i>B. mori</i> midgut total proteins by 2-DE. Molecular mass was indicated on the left and isoelectric point (PI) range on the top. Arrows named A to H in A) and B) referred to the detected spots on PVDF membrane and the corresponding proteins in gel, respectively.</p

Real-time PCR analysis of expression profiles of BmNPV binding proteins in <i>B. mori</i> midguts.

<p>Columns with different colors indicated the experimental treats to the larvae. A to L refer to relative expression level of ATP-A, ATP-B, AK, RP0, Actin, En, PGK, GDAP, PHB2, HSP60, Crt, and RCX1 respectively. Data were normalized using<i>Bmrps3</i>and represented as means±standard errors of the means from three independent experiments. ** Indicates statistical significance<0.01(ANOVA and LSD aposteriori test).</p

The traffic state trend of a link in different seven days.

<p>The traffic state trend of a link in different seven days.</p

RMSE of traffic state estimation by using TDI, BPCA and PPCA.

<p>RMSE of traffic state estimation by using TDI, BPCA and PPCA.</p

The simulated test site.

<p>The simulated test site.</p

Virus binding experiment on lipid-associated and hydrophilic proteins of <i>B. mori</i> midgut resolved by SDS-PAGE.

<p>A) Virus overlay binding experiment. B) Separation of <i>B. mori</i> midgut lipid-associated and hydrophilic proteins by SDS-PAGE. M indicated the standard prestained protein molecular weight marker, NPV was set as positive control, Supernatants meant hydrophilic proteins. Arrows named d, e in A) and B) referred to the detected bands on PVDF membrane and the corresponding proteins in gel.</p

Virus binding experiment on total proteins of <i>B. mori</i> midgut resolved by SDS-PAGE.

<p>A) Virus overlay binding experiment. B) Separation of <i>B. mori</i> midgut total proteins by SDS-PAGE. M indicated the standard prestained protein molecular weight marker (Thermo Scientific), NPV was set as positive control, Midgut referred to <i>B. mori</i> midgut total proteins separated by SDS-PAGE, and the right lane in A) was the negative control that overlaid in binding buffer without BmNPV particles before incubated with monoclonal antibodies against baculovirus gp64. The plus and minus signs on the top meant membranes incubated with or without BmNPV particles. Arrows named a, b, c in A) and B) referred to the detected bands on PVDF membrane and the corresponding proteins in gel.</p