Data_Sheet_1_COVID-19 symptoms and compliance: The mediating role of fundamental social motives.ZIP

BackgroundUnderstanding the compliance of infected individuals and the psychological process underlying compliance during pandemics is important for preventing and controlling the spread of pathogens. Our study investigated whether fundamental social motives mediate the relationship between having infectious disease and compliance.MethodsAn online survey was conducted in March 2020, during the severe phase of the COVID-19 outbreak in China to collect data from 15,758 participants. The survey comprised self-report questionnaires with items pertaining to current symptoms (COVID-19 symptoms, other symptoms or no symptoms), the Fundamental Social Motive Inventory, and measures of compliance. Correlation analysis, linear regression analysis, and structural equation model were used for data analysis.ResultsThe participants with COVID-19 symptoms had lower levels of compliance than those without symptoms, and their lower compliance was caused by a decrease in disease avoidance (indirect effect = −0.058, 95% CI = [−0.061, −0.056]) and familial motives (indirect effect = −0.113, 95% CI = [−0.116, −0.062]). Whereas exclusion concern (indirect effect = 0.014, 95% CI = [0.011, 0.017]) suppressed the effects of COVID-19 symptoms on compliance, the effect disappeared in the multiple mediation model, while those of disease avoidance and familial motives remained.ConclusionOur findings emphasize the critical role of disease avoidance and familial motives in promoting compliance with public health norms during pandemics and suggest that enhancing these motives may serve as an effective intervention strategy to mitigate noncompliance among potentially infected individuals.</p

Molecular Evidence of Glycosylation Effect on the Peptide Assemblies Identified with Scanning Tunneling Microscopy

Glycosylation not only plays a functional role in biological events, but also significantly affects physicochemical properties of proteins. Glycoprotein MUC1 with a variable number of tandem repeats (VNTRs) serves as a promising target for immunotherapy of epithelial cancer. Herein, we synthesized the pristine VNTR and glycosylated VNTR with T antigen functionalized Thr₉ and Tn antigen modified Ser₁₅, involving both disaccharide and monosaccharide. The pristine peptides and glycopeptides are observed to form homogeneous assemblies on the highly oriented pyrolytic graphite surfaces by using scanning tunneling microscopy. These peptide assemblies down to the molecular level demonstrate pronounced site-specific instability induced by glycosylation on graphite surface. Moreover, it can be recognized that disaccharide exerts greater influence on the stability of peptide assemblies than monosaccharide. These results could contribute to the structural insights of glycoprotein and pertinent design of biological applications

Uncovering ceRNA integrated networks that associate with fertility in a photoperiod and temperature sensitive male sterile wheat line

The pollen fertility of photoperiod/temperature sensitive genic male sterile (P/TGMS) wheat is controlled by light and/or temperature. Circular RNA (circRNA) and long non-coding RNA (lncRNA) are known to participate in the development of anthers in plants, but their impact on male sterility in the P/TGMS line is not well understood. In this study, we carried out high-throughput sequencing to investigate the differential expression of lncRNAs and circRNAs and their biological functions in anthers of photo-thermosensitive genic male sterile (PTGMS) wheat line BS366-42L during the transition phase of male fertility under four different photoperiod and temperature treatments. Eight lncRNAs, 40 mRNAs and three circRNAs were screened out and thought as essential candidates that closely related to male sterility. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses were performed to predict the potential functions of differentially expressed RNAs. The results indicated that carbohydrate-related metabolism was important for male sterility in the wheat PTGMS line BS366-42L. lncRNA/circRNA-mRNA-miRNA (ceRNA) integrate networks were constructed to reflect their complex inner association with male sterility. Our study provides a systematic perspective on the potential function of RNAs in male fertility in PTGMS lines of wheat.</p