Additional file 2 of Whole-genome resequencing of wild and cultivated cannabis reveals the genetic structure and adaptive selection of important traits

Additional file 2: Table S2. Sequencing reads and mapping rate of 21 cannabis accessions

Additional file 5 of Whole-genome resequencing of wild and cultivated cannabis reveals the genetic structure and adaptive selection of important traits

Additional file 5: Table S5. Positive selection genes identified by Fst and Δπ in cultivated cannabis accessions (Group 1 and Group 2) by comparison of cultivated cannabis and wild cannabis accessions (Group 3 and Group 4)

Additional file 7 of Whole-genome resequencing of wild and cultivated cannabis reveals the genetic structure and adaptive selection of important traits

Additional file 7: Fig. S1. Analysis of the differences in the main phenotypic characteristics between wild cannabisand cultivated cannabis. The data represent the means ± SDs. Significant differences were determined using GraphPad Prism 8 software (* indicates P <0.05; ** indicates P < 0.01; *** indicates P < 0.001; **** indicates P< 0.0001)

Additional file 3 of Whole-genome resequencing of wild and cultivated cannabis reveals the genetic structure and adaptive selection of important traits

Additional file 3: Table S3. Distribution of SNPs across the whole genome of 52 cannabis accessions

Additional file 4 of Whole-genome resequencing of wild and cultivated cannabis reveals the genetic structure and adaptive selection of important traits

Additional file 4: Table S4. The nucleotide diversities of 52 samples

Additional file 6 of Whole-genome resequencing of wild and cultivated cannabis reveals the genetic structure and adaptive selection of important traits

Additional file 6: Table S6. The primers and probes used in this study

Additional file 9 of Whole-genome resequencing of wild and cultivated cannabis reveals the genetic structure and adaptive selection of important traits

Additional file 9: Fig. S3. Expression of FT-like in wild (W4) and cultivated (C4) cannabis accessions grown underLD conditions at different time points on the same day. Thephotoperiod was set such that it was 18 h of light/6 h of darkness (6:00-24:00for light). Samples were taken every three hours. The sampling location was thefirst to second pair of true leaves, from the top down

Additional file 8 of Whole-genome resequencing of wild and cultivated cannabis reveals the genetic structure and adaptive selection of important traits

Additional file 8: Fig. S2. Summary of nucleotide diversity and correlations between latitude and diversity among cultivated cannabis accessions. A Nucleotide diversity calculated for each individual and plotted based on different groups interms of population structure. The boxes and inside lines represent quartile ranges and median values, respectively. B Scatterplot and linear fitting curve of the latitude and diversity of 13 cultivated cannabis varieties (C1-C6, C9-C12, YNN, GXI and SCN), with some admixed samples removed. C Correlations between latitude and diversity among the 13 cultivated cannabis accessions

Additional file 1 of Whole-genome resequencing of wild and cultivated cannabis reveals the genetic structure and adaptive selection of important traits

Additional file 1: Table S1. Morphological and agronomic characteristics of 21 cannabis accessions in Kunming

Data_Sheet_1_The Impact of Renal Denervation on the Progression of Heart Failure in a Canine Model Induced by Right Ventricular Rapid Pacing.pdf

Heart failure (HF) has been proposed as a potential indication of renal denervation (RDN). However, the mechanisms enabling RDN to attenuate HF are not well understood, especially the central effects of RDN. The aim of this study was to decipher the mode of operation of RDN in the treatment of HF using a canine model of right ventricular rapid pacing-induced HF. Accordingly, 24 Chinese Kunming dogs were randomly grouped to receive sham procedure (sham-operated group), bilateral RDN (RDN group), rapid pacing to induce HF (HF-control group), and bilateral RDN plus rapid pacing (RDN + HF group). Echocardiography, plasma brain natriuretic peptide (BNP), and norepinephrine (NE) concentrations of randomized dogs were measured at baseline and 4 weeks after interventions, followed by histological and molecular analyses. Twenty dogs completed the research successfully and were enrolled for data analyses. Results showed that the average optical density of renal efferent and afferent nerves were significantly lower in the RDN and RDN + HF groups than in the sham-operated group, with a significant reduction of renal NE concentration. Rapid pacing in the RDN + HF and HF-control groups, compared with the sham-operated group, induced a significant increase in left ventricular end-diastolic volume and decrease in left ventricular ejection fraction and correspondingly resulted in cardiac fibrosis and dysfunction. Cardiac fibrosis evaluated by Masson’s trichrome staining and the expression of transforming growth factor-β1 (TGF-β1) were significantly higher in the HF-control group than in the sham-operated group, which were remarkably attenuated by the application of the RDN technique in the RDN + HF group. In terms of central renin–angiotensin system (RAS), the expression of angiotensin II (AngII)/angiotensin-converting enzyme (ACE)/AngII type 1 receptor (AT1R) in the hypothalamus of dogs in the HF-control group, compared with the sham-operated group, was upregulated and that of the angiotensin-(1-7) [Ang-(1-7)]/ACE2 was downregulated. Furthermore, both of them were significantly attenuated by the RDN therapy in the RDN + HF group. In conclusion, the RDN technique could damage renal nerves and suppress the cardiac remodeling procedure in canine with HF while concomitantly attenuating the overactivity of central RAS in the hypothalamus.</p