The changes in BFs (A) and response magnitudes (B) of CN neurons induced by ES_AI as the function of BF differences between the stimulated AI and measured CN neurons.

<p>These changes of BF-matched and BF-unmatched CN neurons were clearly different. The line in A is a regression line. Open circles represent data sampled with protocol 1 and filled circles represent data sampled with protocol 2. The grey boxes and bars in B represent the mean ± SD of percentage changes in spike number following ES_AI.</p

Time required for 10% change, maximum changes, and 50% recovery in response latencies and magnitudes of the BF-matched and BF-unmatched neurons after ES_AI.

<p>Data presented were sampled with protocol 1. *: <i>p</i><0.001, compared between matched and unmatched neurons.</p

Changes in response latencies and magnitudes of BF-matched (A1 and A2) and BF-unmatched (B1 and B2) CN neurons to the time interval from onset of the ES_AI.

<p>Data presented were sampled with protocol 1. The grey bars represent ES_AI.</p

Effects of electrical stimulation of non-AI cortical area on the auditory responses of CN neuron.

<p><b>A</b>. Raster plot of typical changes in auditory responses of a CN neuron before, during and after electrical stimulation. <b>B</b>. Averaged spike numbers calculated within a 10-minute window during the observation period. <b>C</b>. Averaged response latencies calculated within a 10-minute window during the observation period. Arrowheads indicate supplementary injection of ketamine and xylazine. ES: electrical stimulation. N: no electrical stimulation.</p

Effects of sham stimulation of the AI on the auditory responses of CN neuron.

<p><b>A</b>. Raster plot of typical changes in auditory responses of a CN neuron when a stimulating electrode was placed in the AI but no electrical stimulation was given. <b>B</b>. Averaged spike numbers calculated within a 10-minute window during the observation period. <b>C</b>. Averaged response latencies calculated within a 10-minute window during the observation period. The asterisk represents the BF of the AI neuron where the electrode was placed. Arrowheads indicate supplementary injection of ketamine and xylazine. N: no electrical stimulation.</p

Effect of ES_AI on the auditory responses of a BF-matched CN neuron sampled with protocol 1.

<p><b>A</b>. Raster plot of typical changes in auditory responses of a CN neuron with the same BF as that of AI neuron before, during, and after ES_AI. The responses to 19–20 kHz tone markedly increased following ES_AI. <b>B–D</b>. Changes in the latencies of this neuron in response to 19 kHz, 20 kHz and 21 kHz tones following ES_AI. The response latencies were reduced by ES_AI. The asterisk represents the BF of the stimulated AI neurons. The grey lines in B–D represent ES_AI.</p

Effect of ES_AI on the auditory responses of a BF-unmatched CN neuron sampled with protocol 1.

<p><b>A</b>. Raster plotting of a typical change in auditory responses of a CN neuron that had a BF different from that of AI neurons before, during, and after ES_AI. The responses to 12–13 kHz decreased and then ceased whereas the responses to 14 kHz appeared and gradually increased following electrical stimulation of AI neuron tuned 16 kHz. <b>B–D</b>. Changes in the latencies of this neuron in response to 12 kHz, 13 kHz and 14 kHz tones following ES_AI. The response latencies to 12–13 kHz tones gradually increased before the cessation of auditory responses and recovered with extremely long latencies. The changes in response latencies were similar to that of BF-matched neurons depicted in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0014038#pone-0014038-g001" target="_blank">Fig. 1</a>. The asterisk represents the BF of the stimulated AI neurons. The grey lines in B–D represent ES_AI.</p

Additional file 1 of The characteristics and prognosis of different disease patterns of multiple primary lung cancers categorized according to the 8th edition lung cancer staging system

Supplementary Material