Additional file 1 of The association between ambient air pollution and scarlet fever in Qingdao, China, 2014–2018: a quantitative analysis

Additional file 1: Results of spearman correlation and sensitivity analysis

Patient characteristics and Univariate analysis (n = 446).

a<p>Tumor differentiation according to the WHO classification for gastric cancer in 2000; ^bTNM stage according to TNM 7th edition by AJCC(American Joint Committee on Cancer); NA Not arrival; *<i>P</i><0.05.</p

Immunohistochemical staining of TGF-β, HIF-1α, VEGF and pERK 1/2 expression in gastric cancer.

<p>A. Immunohistochemical staining of TGF-β was located mainly in the cytoplasm of tumor cells (positive expression ×400); B. TGF-β original magnification ×100; C. HIF-1α was located mainly in the nucleus of tumor cells (positive expression ×400); D. HIF-1α original magnification ×100; E. VEGF was located mainly in the cytoplasm of tumor cells (positive expression ×400); F. VEGF original magnification×100; G. pERK1/2 was located in the cytoplasm and nucleus of tumor cells (positive expression ×400); H. pERK1/2 original magnification ×100.</p

Two procedures for evaluation of HER-2 expression.

<p>* IRS score (Immunoreactive Score) according to Remmele <i>et al</i> and Halon <i>et al</i><a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0090678#pone.0090678-Remmele1" target="_blank">[8]</a>, <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0090678#pone.0090678-Halon1" target="_blank">[10]</a>.</p

Multivariate analysis of significant prognostic factors for survival in patients with gastric carcinoma.

<p>DFS, Disease-free survival; OS, Overall Survival; HR, hazard ratio; CI, Confidence interval; *P<0.05; 1, A, Adenocarcinoma, S, Absolute signet ring cell carcinoma, M, Mixed carcinoma; 2, I, Intestinal, D, Diffuse, M, Mixed type; 3, P/D, Poor differentiation, M/D, Moderate differentiation, H/D High differentiation; 4, W, Weak-expression, O, Overexpression.</p

Kaplan-Meier curves for disease-free survival.

<p>TGF-β, HIF-1α, VEGF and pERK 1/2 overexpression were divided into an overexpression group and a weak-expression group. A log-rank test was used to calculate significance. A. Disease-free survival curves stratified by TGF-β expression (<i>P</i> = 0.053). B. Disease-free survival curves stratified by HIF-1α expression (<i>P</i> = 0.000). C. Disease-free survival curves stratified by VEGF expression (<i>P</i> = 0.161). D. Disease-free survival curves stratified by pERK 1/2 expression (<i>P</i> = 0.107).</p

TGF-β and HIF-1α expressions and Clinicopathologic characteristics.

a<p>Pearson's Chi-Square test or Fisher's Exact test, P<0.05.</p

VEGF and pERK expressions and Clinicopathologic characteristics.

a<p>Pearson's Chi-Square test or Fisher's Exact test, P<0.05.</p

Kaplan-Meier curves for overall survival.

<p>TGF-β, HIF-1α, VEGF and pERK 1/2 overexpression were divided into an overexpression group and a weak-expression group. A log-rank test was used to calculate significance. A. Overall survival curves stratified by TGF-β expression (<i>P</i> = 0.139). B. Overall survival curves stratified by HIF-1α expression (<i>P</i> = 0.000). C. Overall survival curves stratified by VEGF expression (<i>P</i> = 0.217). D. Overall survival curves stratified by pERK 1/2 expression (<i>P</i> = 0.018).</p