Sampling sites of 16 orchards.

<p>The blue dash lines on the map are the administrative boundaries of Mei county and Zhouzhi county which located on the middle of Shaanxi province of China. The plain between the Weihe River and the Tsinling Mountains within these two counties, which is a part of the Guanzhong Plain, is the main region of kiwi fruit production in Shaanxi province.</p

The number of samples and positive samples of the 16 orchards and their geographic coordinate.

<p>The number of samples and positive samples of the 16 orchards and their geographic coordinate.</p

Changes in microbial populations during the 45°C incubation.

<p>A, pH adjusted kiwi fruit juice inoculated with 4 strains of <i>Alicyclobacillus</i>; B, kiwi fruit juice inoculated with 4 strains of <i>Alicyclobacillus</i>; C, kiwi fruit juice inoculated with 4 strains of <i>Alicyclobacillus</i> (with a heat shock treatment); D, six kinds commercial kiwi fruit products inoculated with C-ZJB-12-35.G.</p

The number and the sampling and enrichment methods of the samples from the producer.

<p>The number and the sampling and enrichment methods of the samples from the producer.</p

All isolated strains, their sources, number of nucleotides sequenced, nearest phylogenetic neighbour and similarity.

<p>All isolated strains, their sources, number of nucleotides sequenced, nearest phylogenetic neighbour and similarity.</p

Neighbour-joining phylogenetic tree of isolates and reference species.

<p>The 4 reference species are <i>Alicyclobacillus acidoterrestris</i> DSM 3923, <i>Alicyclobacillus cycloheptanicus</i> DSM 4006, <i>Alicyclobacillus contaminans</i> DSM 17975, and <i>Alicyclobacillus herbarius</i> DSM 13609. Construction is based on 16S rRNA gene sequence comparisons. Bootstrap percentages based on 1000 replicates are shown. Bar, p-distance (0.02 substitutions per nucleotide position).</p

Electrophoresis patterns of RAPD-PCR of the genomic DNA of isolates and reference species.

<p>The 4 reference species are <i>Alicyclobacillus acidoterrestris</i> DSM 3923, <i>Alicyclobacillus cycloheptanicus</i> DSM 4006, <i>Alicyclobacillus contaminans</i> DSM 17975, <i>Alicyclobacillus herbarius</i> DSM 13609. I–VI indicate groups within a certain species. Marker, DNA marker DL2000 (BGI). A, Primer A-01; B, Primer AZ-14.</p

The peak areas of guaiacol of samples and the calculated concentrations.

<p>The peak areas of guaiacol of samples and the calculated concentrations.</p

A GC–MS chromatogram of all standard solutions and a standard curve generated by Excel.

<p>For comparison purposes only peaks between the mass range of 81, 109, 124 m/z are displayed.</p

A GC–MS chromatogram of pH adjusted kiwi fruit juice inoculated with C-ZJB-12-35.

<p>Mass spectrums from the peak (14.47 min) thought to be guaiacol in this sample and from standard guaiacol are also shown. The compound peaked at 281 m/z may come from the SPME fiber, because it exist in the peak around 14.47min in all samples. Because the scanning mass range was 50–350 m/z, peaks below 50 m/z were not included. Both of these may reduce the SI and RSI values.</p