Supplemental Material - How to Engage Employees in Corporate Social Responsibility? Exploring Corporate Social Responsibility Communication Effects Through the Reasoned Action Approach

Supplemental Material for How to Engage Employees in Corporate Social Responsibility? Exploring Corporate Social Responsibility Communication Effects Through the Reasoned Action Approach by Chuqing Dong, Yafei Zhang, and Song Ao in Management Communication Quarterly</p

PHYSIOLOGICAL AND BIOCHEMICAL MONITORING IN DANCESPORT ATHLETES

ABSTRACT Introduction The dancesport is an emerging sports competition that integrates competitive sports and dance; college students have been using the training for aesthetic purposes in body delineation. However, the biochemical alterations are still underexplored. Objective Explore the physiological and biochemical changes caused by dancesport. Methods The volunteers were submitted to a two-week high-intensity dancesport protocol. Changes in physiological and biochemical indicators were monitored before and after the experiment. Results The athletes exhibited individual changes in biochemical indicators (P</div

DataSheet_1_Development and evaluation of 4WSS electric-driven chassis for high-clearance sprayer.zip

IntroductionThe high clearance sprayer with conventional steering mechanisms, as an intelligent spraying machine, is frequently stuck or broken in muddy fields due to the excessive torque load.MethodsA Four-Wheel Self-Steering (4WSS) electric-driven chassis with a smaller turning radius and better passability is developed to handle complex agricultural terrains. The 4WSS chassis is mainly composed of two custom-designed steering bridges and four in-wheel drive motors. It can achieve steering and driving forward simultaneously through coordinate differential speed control of drive motors, saving a set of dedicated servo steering systems and requiring less torque during steering compared to conventional structures. A kinematic model depicting the speed relationships between four wheels is established via geometric analysis, and a Speed Distribution Controller (SDC) is designed to accomplish locomotion objectives.ResultsExperimental results demonstrate the effectiveness of the new prototype 4WSS chassis system in tracking speed and steering angle. Compared to conventional agricultural chassis, the 4WSS chassis has a smaller turning radius of 2,877 mm. DiscussionThe 4WSS chassis exhibits superior performance in typical field conditions, including muddy terrain, deep gullies, and ridges.</p

MOESM1 of Screening for functional IRESes using α-complementation system of β-galactosidase in Pichia pastoris

Additional file 1. Sequences of IRES

MOESM2 of Screening for functional IRESes using α-complementation system of β-galactosidase in Pichia pastoris

Additional file 2. Additional Table and Figure

Effect of Stac3 knockdown on proliferation and differentiation of C2C12 myoblasts.

<p>C2C12 myoblasts were transfected with a pool of three Stac3 siRNAs or a scrambled siRNA and were expanded in growth medium or induced to differentiate into myotubes in differentiation medium. (<b>A</b>) Relative expression levels of Stac3 mRNA in C2C12 cells transfected with Stac3 siRNAs or scrambled siRNA at 48 h after siRNA transfection. (<b>B</b>) Proliferation rate of C2C12 cells transfected with Stac3 siRNAs or scrambled siRNA. Cell proliferation rate was estimated by measuring the numbers of viable cells at 0, 24, 48, and 72 h after transfection. The absorbance at 570 nm on the y-axis represents the number of viable cells. The proliferation rate was not different between cells transfected with Stac3 siRNAs and those with scrambled siRNA. (<b>C</b>) Representative images of C2C12 cells transfected with Stac3 siRNAs or scrambled siRNA at 72 h of differentiation. The cells were stained with Giemsa. (<b>D</b>) Fusion index at 72 h of differentiation. Fusion index was calculated as the percentage of total nuclei that resided in cells containing 3 or more nuclei. (<b>E</b>) Average number of nuclei per myotube at 72 h of differentiation. (<b>F</b>) Relative expression levels of myogenin (Myog), myosin heavy chain 3 (Myh3), myoglobin (Mb), troponin T type 1 (Tnnt1) and 3 (Tnnt3), and creatine kinase, muscle (Ckm) mRNAs at 0, 24, 48, and 72 h of differentiation. (<b>G</b>) Western blots of myosin heavy chain (MHC), myogenin (MyoG), and β-actin (loading control) proteins at 0, 24, 48, and 72 h of differentiation. Data are expressed as means ± s.e.m. (n  =  4 independent cell cultures). * <i>P</i><0.05; ** <i>P</i><0.01 versus “Scrambled siRNA”.</p

Additional file 1 of Exploring the key genomic variation in monkeypox virus during the 2022 outbreak

Additional file 1. Details of 954 MPXV isolates from the NCBI database

Effect of Stac3 overexpression on proliferation and differentiation of C2C12 myoblasts.

<p>C2C12 myoblasts were transfected with the Stac3-Flag fusion protein expression plasmid pcDNA3.1/Stac3-Flag or empty vector pcDNA3.1, and were expanded in growth medium or induced to differentiate into myotubes in differentiation medium. (<b>A</b>) Relative expression levels of Stac3 mRNA in C2C12 cells at 48 h after transfection. (B) Expression levels of Stac3-Flag fusion protein in C2C12 cells at 48 h after transfection. The Stac3-Flag fusion protein was detected by anti-Flag antibody and indicated by an arrow. (<b>C</b>) Proliferation rate of C2C12 cells transfected with pcDNA3.1/Stac3-Flag or pcDNA3.1. The proliferation rate was not different between cells transfected with pcDNA3.1/Stac3-Flag and pcDNA3.1. (<b>D</b>) Representative images of transfected C2C12 cells at 72 h of differentiation. (<b>E</b>) Fusion index of transfected C2C12 cells at 72 h of differentiation. (<b>F</b>) Average number of nuclei per myotube at 72 h of differentiation. (<b>G</b>) Relative expression levels of myogenin (Myog), myosin heavy chain 3 (Myh3), myoglobin (Mb), troponin T type 1 (Tnnt1) and 3 (Tnnt3), and creatine kinase, muscle (Ckm) mRNAs at 0, 24, 48, and 72 h of differentiation. (<b>H</b>) Western blots of myosin heavy chain (MHC), myogenin (MyoG), and β-actin (loading control) proteins at 0, 24, 48, and 72 h of differentiation. Data are expressed as means ± s.e.m. (n  =  4 independent cell cultures). * <i>P</i><0.05; ** <i>P</i><0.01 versus “pcDNA3.1”.</p

Additional file 5 of Establishment of a multi-parameter prediction model for the functional cure of HBeAg-negative chronic hepatitis B patients treated with pegylated interferonα and decision process based on response-guided therapy strategy

Table S3 Weighted Kappa consistency test between baseline and 12W