Additional file 1 of IL13Rα2 as a crucial receptor for Chi3l1 in osteoclast differentiation and bone resorption through the MAPK/AKT pathway

Additional file 1: Supplemental Figure 1. Neither recombinant Chi3l1/sIL13Rα2protein nor Chi3l1/sIL13Rα2 silencing had any effect on the proliferative ability of BMMs. A BMMs were treated with M-CSF and various concentrations of chi31l recombinant protein (0, 125, 250, 500, 1000ng/ml) for 48h and 96h, then the proliferative ability of BMMs was determined by the CCK-8 experiment. B After silencing Chi3l1or IL13Rα2 expression in BMMs with siRNA for 48h, BMMs were treated with M-CSF for 48h and 96h, then the proliferative ability of BMMs was determined by the CCK-8 experiment. C BMMs were treated with M-CSF and various concentrations of rIL13Rα2 protein (0, 125, 250, 500, 1000ng/ml) for 48h and 96h, then the proliferative ability of BMMs was determined by the CCK-8 experiment. **P < 0.01. Results are expressed as means ± SE

Presentation1_Titanium Nanobowl-Based Nest-Like Nanofiber Structure Prepared at Room Temperature and Pressure Promotes Osseointegration of Beagle Implants.pdf

Nest-like nanofiber structures have potential applications in surface modifications of titanium implants. In this study, nest-like nanofiber structures were prepared on a titanium surface at room temperature and pressure by using the nanobowl template-assisted method combined with alkali etching. The characterization and biocompatibility of this material were analyzed by cellular adhesion, death, CCK-8, ALP, and RT-PCR assays in vitro, and osseointegration was evaluated by micro-CT and fluorescent labeling in vivo. The results showed that this nest-like nanofiber structure has a firmer and asperate surface than nanotubes, which leads to better cellular adhesion, proliferation, and differentiation capacity. In a beagle alveolar bone implant model, the nest-like nanofiber structure showed a better osseointegration capacity. In conclusion, this nest-like nanofiber structure has potential applications in dental implantology.</p