DataSheet1_UroVysion™ fluorescence in situ hybridization (FISH) possibly has a high positive rate in carcinoma of non-urothelial lineages.PDF

Background: Positive UroVysion™ fluorescence in situ hybridization (FISH) is generally considered as urothelial carcinoma (UC). We clarify if UroVysion™ FISH can be positive in carcinoma of non-urothelial lineages (CNUL), and verify the consistency of urine FISH and histological FISH in CNUL.Methods: All CNUL subjects detected by urine FISH assay due to haematuria from Tongji Hospital were screened. Meanwhile, 2 glandular cystitis and 2 urothelial carcinoma were served as negative or positive control. Paraffin-embedded tissue sections of all subjects were sent to the pathology department for histological FISH detection.Results: A total of 27 patients were included in this study, including 9 with adenocarcinomas, 11 with squamous cell carcinomas, and 7 with other tumour types. The overall positive rate in urine FISH was 64.00% (16/25) in patients with CNUL, 77.78% (7/9) in those with adenocarcinoma and 54.55% (6/11) in those with squamous carcinoma. There was a significant difference in the GLP p16 gene deletion rate between UC and CNUL (100% vs. 8.00%, p = 0.017). Histological FISH results showed that the histological results of 19 patients were consistent with their urine FISH results, and only one patient with stage Ⅲa urachal carcinoma had inconsistent histological FISH results (positive) and urine FISH (negative) results.Conclusion: We demonstrated for the first time the application value of FISH in CNUL on urine samples. Positive urine FISH tests indicate not only UC, but also CNUL. UroVysion™ FISH possibly has a high positive rate in CNUL. CNUL and UC have different genetic changes shown by FISH.</p

Additional file 2: of Effect of acupuncture and its influence on cerebral activity in perimenopausal insomniacs: study protocol for a randomized controlled trial

Consent form. (DOC 114 kb

Magnetic and Quantum Transport Properties of Small-Sized Transition-Metal-Pentalene Sandwich Cluster

The chemical bonds and magnetic and quantum transport properties of small-sized transition-metal-pentalene sandwich clusters TM_2<i>n</i>Pn_<i>n</i>+1 (TM = V, Cr, Mn, Co, and Ni; <i>n</i> = 1, 2) were investigated by using density functional theory and nonequilibrium Green’s function method. Theoretical results show that TM_2<i>n</i>Pn_<i>n</i>+1 sandwiches have high stabilities. The TM–TM bond order gradually decreases with the increase of 3d electron number of TM atoms and TM_2<i>n</i>Pn_<i>n</i>+1 could exhibit different spin states. With Au as two electrodes, significant spin-filter capability was observed in TM_2<i>n</i>Pn_<i>n</i>+1, and such a filter can be switched on/off by changing the spin state. In addition, giant magnetoresistance was also found in the systems. These interesting quantum transport properties indicate that TM_2<i>n</i>Pn_<i>n</i>+1 sandwiches are promising materials for designing molecular junction with different functions

Additional file 1: Figure S1. of Expression of amphiregulin predicts poor outcome in patients with pancreatic ductal adenocarcinoma

AREG RNA and protein levels in pancreatic cancer cells and pancreatic stellate cells. A, AREG mRNA levels in pancreatic cancer cells and pancreatic stellate cells. B, AREG protein levels in pancreatic cancer cells and pancreatic stellate cells, * indicates a P < 0.05. (DOC 718 kb

Lewis and Brønsted Acid Synergistic Catalysis for Efficient Synthesis of Hydroxylamine over Heteroatom Zeolites

Environmental and efficient synthesis of NH2OH is challenging. Herein, we have provided a route for efficient NH2OH formation by NH3 oxidation via the synergistic catalysis of Lewis acid sites of the framework Ti (Ti LAS) and Brønsted acid sites of the framework Al (Al BAS) in Ti–Al-MOR zeolites. Ti LAS was the active center for formation of transition-state NH2OH, while Al BAS could provide the proton H to convert transition-state NH2OH absorbed on Ti LAS to a [NH3OH]+ species. Protonation effect would not only promote the catalytic cycle process resulting from accelerating the desorption of transition-state NH2OH, but also improve the stability of NH2OH, being less prone to oxidative decomposition. In this work, we presented the synergistic catalysis between LAS and BAS over Ti-containing heteroatom zeolites to accelerate the catalytic cycle, which provided an environmental and efficient method for NH2OH formation

Synthesis, Characterization, and Properties of Bis-BN Ullazines

A series of bis-BN ullazine derivatives, including the parent species, were synthesized in a small number of steps from commercially available materials. X-ray crystallographic analysis revealed that bis-BN ullazines have rigid and planar frameworks. Most of the bis-BN ullazines are stable toward air and moisture. In addition, the absorption and emission bands of these ullazines are blue-shifted, compared to those of their carbonaceous ullazine analogs

Highly Oriented Thin Membrane Fabrication with Hierarchically Porous Zeolite Seed

Nanosized zeolite is widely used as seed for high quality zeolite membranes fabrication, while its complicated synthesis routine limits large-scale productions. In this work, a non-nanosized cubic hierarchically porous TS-1 zeolite (HTS-1), obtained by basic hydrothermal treatment of conventional ellipsoid solid TS-1, is used as seed to prepare highly oriented thin membranes. A capillary condensation phenomenon resulting from the unique hierarchically porous structure benefits gel attachment. Moreover, abundant ledges, kinks, and terraces on the HTS-1 surface promote epitaxial growth of the membrane. In contrast, the solid TS-1 seed induces intergrowth dominantly, which results in a thick TS-1 membrane. The HTS-1 membrane demonstrates superior CO₂/N₂ separation properties compared to the TS-1 one. It associates with thin oriented membrane morphology, leading to exposure of a high Miller index surface and less diffuse distance and tortuosity. The results suggest beneficial effects of a hierarchically porous TS-1 zeolite seed on the interfacial crystal growth for membrane fabrication. A similar conclusion is applicable to the case of a hierarchically porous zeolite β. This work develops a facile approach to obtain a highly oriented thin zeolite membrane with enhanced separation properties

Preparation and Evaluation of Water-Compatible Surface Molecularly Imprinted Polymers for Selective Adsorption of Bisphenol A from Aqueous Solution

Water-compatible molecularly imprinted polymers (MIPs) for adsorbing bisphenol A (BPA) in aqueous solutions are synthesized using water-soluble monomer as surface hydrophilicity-increasing agent via surface addition–fragmentation chain transfer polymerization. The formation and structure of these hybrid materials are verified by Fourier transform infrared spectroscopy, contact angle studies, thermogravimetric analysis, and scanning electron microscopy. The characterization and adsorption results indicate that the molecularly imprinted polymers prepared with 2-acrylamido-2-methylpropanesulfonic acid (AMPS/MIPs) are water-compatible (the contact angle is 14°). The excellent dispersion of AMPS/MIPs in water provides more opportunity for BPA molecules to access the imprinted cavities and improves their recognition characteristics. The kinetics and isotherm data of AMPS/MIPs can be well described by the pseudo-second-order kinetic model and the Langmuir isotherm, respectively. The thermodynamic studies indicate that the adsorption process is a spontaneous exothermic process

Immobilization of Highly Dispersed Ag Nanoparticles on Carbon Nanotubes Using Electron-Assisted Reduction for Antibacterial Performance

Silver nanoparticles (Ag NPs) supported on certain materials have been widely used as disinfectants. Yet, to date, the antibacterial activity of the supported Ag NPs is still far below optimum. This is mainly associated with the easy aggregation of Ag NPs on the supporting materials. Herein, an electron-assisted reduction (EAR) method, which is operated at temperatures as low as room temperature and without using any reduction reagent, was employed for immobilizing highly dispersed Ag NPs on aminated-CNTs (Ag/A-CNTs). The average Ag NPs size on the EAR-prepared Ag/A-CNTs is only 3.8 nm, which is much smaller than that on the Ag/A-CNTs fabricated from the traditional thermal calcination (25.5 nm). Compared with Ag/A-CNTs fabricated from traditional thermal calcination, EAR-prepared Ag/A-CNTs shows a much better antibacterial activity to <i>E. coli</i>/<i>S. aureus</i> and antifouling performance to <i>P. subcordiformis</i>/<i>T. lepidoptera</i>. This is mainly originated from the significantly enhanced Ag⁺ ion releasing rate and highly dispersed Ag NPs with small size on the EAR-prepared Ag/A-CNTs. The findings from the present work are helpful for fabricating supported Ag NPs with small size and high dispersion for efficient antibacterial process

Power Conversion Efficiency and Device Stability Improvement of Inverted Perovskite Solar Cells by Using a ZnO:PFN Composite Cathode Buffer Layer

We have demonstrated in this article that both power conversion efficiency (PCE) and performance stability of inverted planar heterojunction perovskite solar cells can be improved by using a ZnO:PFN nanocomposite (PFN: poly­[(9,9-bis­(3′-(<i>N</i>,<i>N</i>-dimethylamion)­propyl)-2,7-fluorene)-<i>alt</i>-2,7-(9,9-dioctyl)-fluorene]) as the cathode buffer layer (CBL). This nanocomposite could form a compact and defect-less CBL film on the perovskite/PC₆₁BM surface (PC₆₁BM: phenyl-C₆₁-butyric acid methyl ester). In addition, the high conductivity of the nanocomposite layer makes it works well at a layer thickness of 150 nm. Both advantages of the composite layer are helpful in reducing interface charge recombination and improving device performance. The power conversion efficiency (PCE) of the best ZnO:PFN CBL based device was measured to be 12.76%, which is higher than that of device without CBL (9.00%), or device with ZnO (7.93%) or PFN (11.30%) as the cathode buffer layer. In addition, the long-term stability is improved by using ZnO:PFN composite cathode buffer layer when compare to that of the reference cells. Almost no degradation of open circuit voltage (<i>V</i>_OC) and fill factor (FF) was found for the device having ZnO:PFN, suggesting that ZnO:PFN is able to stabilize the interface property and consequently improve the solar cell performance stability