High expression of lncRNA HOTAIR in cervical cancer tissues is associated with patients with tumor node metastasis (TNM) staging.

<p><b>(A)</b> HOTAIR expression was classified into two groups based on median value of HOTAIR expression in cervical cancer tissues. <b>(B)</b> The HOTAIR expression was significantly higher in patients with advanced pathological stage (II+III+IV) than those with lower pathological stage (0+I).</p

Distribution of allele/genotype frequency of three haplotype-tagging SNPs in HOTAIR and their association with cervical cancer risk.

<p>Distribution of allele/genotype frequency of three haplotype-tagging SNPs in HOTAIR and their association with cervical cancer risk.</p

LncRNA-HOTAIR rs920778 SNP T variant allele exhibits enhanced enhancer activity and increases HOTAIR expression.

<p><b>(A)</b> Enhancer activity are presented as fold increase relative to negative control (NC, empty vector PGL3). “**” indicates <i>P</i><0.01. Individuals with the risk allele T at rs920778 in both 38 normal cervical tissues <b>(B)</b> and 43 cervical cancer tissues <b>(C)</b> is significantly associated with increased HOTAIR expression than those with the CC genotypes. Expression levels of HOTAIR are detected by RT-PCR in cervical cancer tissues grouped to three genotypes (rs920778TT, rs920778CT or rs920778CC). “*” indicates <i>P</i><0.05. <b>(D)</b> RT-PCR analysis of HOTAIR expression levels in cervical cancer cell lines (SiHa, Hela and Caski) compared with breast cancer cell MCF-7 and human embryo kidney epithelial cell 293T. <b>(E)</b> The HOTAIR expression level was analyzed by RT-PCR in 91 cervical cancer tissue samples. HOTAIR expression levels was significantly upregulated in cervical cancer tissues compared with their matched non-tumor cervical cancer tissues (<i>P</i><0.05). T, tumor; N, no-tumor.</p

Demographic characteristic of patients with cervical cancer and controls.

<p>Demographic characteristic of patients with cervical cancer and controls.</p

Stratified analyses of lncRNA HOTAIR polymorphisms with clinical characteristics of patients with cervical cancer.

<p>Stratified analyses of lncRNA HOTAIR polymorphisms with clinical characteristics of patients with cervical cancer.</p

Origin of ihBTCs.

<p>A, Under the in vivo fluorescence imaging system, SU3-RFP xenotransplanted tumors in the right caudate nucleus of EGFP nude mice were red. B, Red tumor cells interweave with the green host cells in the xenotransplanted tumor frozen section observed under a fluorescent microscope (scale plate 20 μm). C, HE staining of ihBTC cells. D-E, (scale plate of 50 μm) IhBTC cells express EGFP in in vitro culture. Immunofluorescence reveals that ihBTC2 cells highly express the oligodendrocyte marker CNP (in red and DAPI in blue). F, Karyotype analysis indicates that ihBTC2 cells are hyperpentaploid and all chromosomes are monocentric. G and H, Large tumor formed in non-fluorescent nude mice after subcutaneous injection of ihBTC2 cells. H&E staining of the tumors showed the malignant tumor features of poor differentiation, pleomorphism, hyperchromatic nuclei, loss of polarity and local invasion (200×).</p

Radiosensitivity of ihBTC2 is improved by inhibiting the Notch signaling pathway.

<p>A-B, The cell proliferation (A) and the colony forming ability (B) of the radiation combined with GSI group is decreased compared to the radiation group (P<0.05). C, The SF2 of the untreated and GSI-treated ihBTC2 cells were 0.57±0.05 and 0.75±0.03 (P<0.05), respectively. D-E, Compared to the radiation group, the caspase-3/7 activity of the radiation combined with GSI group was significantly increased after 48 hours (D) and 72 hours (E). F, Trypan blue staining indicated that the dead cell rate of the radiation combined with GSI group was significantly higher compared to the radiation group after 96 hours (P<0.05). G, After 24 hours, the Annexin V staining of the blank, GSI, radiation and radiation combined with GSI group were 0.22%±0.04%, 0.29%±0.04%, 4.60±0.43% and 11.68±0.52%, respectively.</p

sj-docx-1-tam-10.1177_17588359211054895 – Supplemental material for Comprehensive analysis of prognostic value of lymph node classifications in esophageal squamous cell carcinoma: a large real-world multicenter study

Supplemental material, sj-docx-1-tam-10.1177_17588359211054895 for Comprehensive analysis of prognostic value of lymph node classifications in esophageal squamous cell carcinoma: a large real-world multicenter study by Junmiao Wen, Jiayan Chen, Donglai Chen, Salma K. Jabbour, Tao Xue, Xufeng Guo, Haitao Ma, Fei Ye, Yiming Mao, Jian Shu, Yangyang Liu, Xueguan Lu, Zhen Zhang, Yongbing Chen and Min Fan in Therapeutic Advances in Medical Oncology</p

Cell radiation dose-survival curve.

<p>The survival rate of the four cell lines decreases as the radiation dose increases in a dose-dependent manner. For radiation resistance, ihBTC2, SU3-5R, C6 and SU3 cells are in descending order.</p

sj-docx-2-tam-10.1177_17588359211054895 – Supplemental material for Comprehensive analysis of prognostic value of lymph node classifications in esophageal squamous cell carcinoma: a large real-world multicenter study

Supplemental material, sj-docx-2-tam-10.1177_17588359211054895 for Comprehensive analysis of prognostic value of lymph node classifications in esophageal squamous cell carcinoma: a large real-world multicenter study by Junmiao Wen, Jiayan Chen, Donglai Chen, Salma K. Jabbour, Tao Xue, Xufeng Guo, Haitao Ma, Fei Ye, Yiming Mao, Jian Shu, Yangyang Liu, Xueguan Lu, Zhen Zhang, Yongbing Chen and Min Fan in Therapeutic Advances in Medical Oncology</p