26 research outputs found
Screening and unveiling antibacterial mechanism of dandelion phenolic extracts against <i>Staphylococcus aureus</i> by inhibiting intracellular Na<sup>+</sup>–K<sup>+</sup> ATPase based on molecular docking and molecular dynamics simulation
Staphylococcus aureus is one of the most frequently food-contaminated incidence of healthcare-associated Gram-positive bacteria. The antibacterial function and mechanism of phenolic compounds from dandelion are still unclear. Herein, this work aims to screen one of dandelion phenolic extracts with the strongest antibacterial function from its organ such as flower, stem, leaf and root, and to reveal its antibacterial mechanism. The results indicated dandelion flower phenolic extract (DFPE) containing the highest content of caffeic acid, followed by luteolin and luteolin-7-O-glucoside. They, especially caffeic acid and luteolin-7-O-glucoside, played a key role in making the bacterial cellular-membrane ruptured against the bacteria. The leakage of the intracellular substances (adenosine triphosphate and Na+–K+ ATPase) was further confirmed. Conventional hydrogen bond, pi-anion, pi-alkyl were involved in the interaction between caffeic acid or luteolin-7-O-glucoside and Na+–K+ ATPase. Additionally, the dynamic equilibrium of the liganded ATPase complex were achieved after 105 ns, and the lower values from the radius of gyration and solvent accessible surface area in the complex demonstrated the highly tight and compact structure of the liganded protein. The highest free binding energy (ΔGbind = −47.80 kJ/mol) between Na+–K+ ATPase and luteolin-7-O-glycloside was observed. Overall, DFPE can be used as an effective anti-bacterial agent due to the contribution of its bioactive ingredients such as caffeic acid and luteolin-7-O-glucoside for membrane-breaking. Communicated by Ramaswamy H. Sarma</p
Direct Synthesis of Quinolines via Co(III)-Catalyzed and DMSO-Involved C–H Activation/Cyclization of Anilines with Alkynes
A unique
CoÂ(III)-catalyzed and DMSO-involved C–H activation/cyclization
of simple, cheap, and easily available anilines with alkynes for direct
and highly efficient synthesis of privileged quinolines with exclusive
regioselectivity and broad substrate/functional group tolerance and
in good to excellent yields, where DMSO was employed as both the solvent
and the C<sub>1</sub> building block of quinoline products, is reported.
Mechanistic experiments revealed that the versatile reaction might
employ the 2-vinylbenzenamine species as the active intermediate
Regioselective Synthesis of 2,3,4-Trisubstituted Pyrroles via Pd(II)-Catalyzed Three-Component Cascade Reactions of Amines, Alkyne Esters, and Alkenes
A new,
efficient, and versatile PdÂ(II)-catalyzed oxidative three-component
cascade reaction of diverse amines, alkyne esters, and alkenes is
disclosed for the direct synthesis of diverse 2,3,4-trisubstituted
pyrroles with broad functional group tolerance and in good to excellent
yields. This transformation is supposed to proceed through the cascade
formation of CÂ(sp<sup>2</sup>)–CÂ(sp<sup>2</sup>) and CÂ(sp<sup>2</sup>)–N bonds via PdÂ(II)-catalyzed regioselective alkene
migratory insertion, intramolecular radical addition, and oxidation
sequential processes
Divergent Synthesis of Quinolones and Dihydroepindolidiones via Cu(I)-Catalyzed Cyclization of Anilines with Alkynes
A unique
and efficient method for one-pot synthesis of diverse 4-quinolones
from simple and readily available primary anilines and alkynes via
CuÂ(I)-catalyzed direct cyclization has been developed. The (thio)Âphenols
were also found to visible substrates for this reaction. Moreover,
an unprecedented synthesis of dihydroepindolidiones has been demonstrated
by using secondary anilines as the versatile substrates
Functional characterisation of <i>MdMYB44</i> as a negative regulator in the response to cold and salt stress in apple calli
<p>MYB (v-myb avian myeloblastosis viral oncogene homolog) transcription factors (TFs) participate in the abiotic stress response in several plant species. In this study, we cloned and characterised <i>MdMYB44</i> from apple (<i>Malus domestica</i>). Phylogenetic analysis showed that the MdMYB44 protein was highly homologous to <i>Arabidopsis thaliana</i> MYB44. In addition, MdMYB44 localised to the nuclei in transformed onion epidermal cells and showed transcriptional activation activity in a yeast one-hybrid assay. <i>MdMYB44</i> was expressed in the stem, leaves, roots, flowers, and fruits, with the highest transcript level being observed in the leaves. Quantitative PCR analysis demonstrated that <i>MdMYB44</i> expression was down-regulated by cold, osmotic, and salt stresses. Furthermore, stable overexpression of <i>MdMYB44</i> in transgenic apple calli remarkably alleviated their tolerance to high salinity and cold, and relative conductivity and malondialdehyde accumulation under salt and cold treatments were significantly higher than those in wild-type control calli. Therefore, our findings provide evidence that <i>MdMYB44</i> is involved in susceptibility to cold and salt stress in apple calli and indicate MdMYB44 to negatively regulate plant abiotic stress defence.</p
Data_Sheet_1_Short-term effectiveness of baricitinib in children with refractory and/or severe juvenile dermatomyositis.xlsx
ObjectiveTo determine the short-term effectiveness safety of baricitinib in children with refractory and/or severe juvenile dermatomyositis (rsJDM) in a real-world setting.MethodsThis was a single-center retrospective study, including 20 children with rsJDM. They were all treated using baricitinib combined with steroids and other immunosuppressive agents. The childhood myositis assessment scale (CMAS) and PRINTO remission criteria were used to evaluate the disease severity and treatment outcome at 0, 4, 12, and 24 weeks after initiation of baricitinib.ResultsThe skin rash improved in 95% of patients (19/20) at week 24, with a significant decrease of skin-DAS at weeks 12 (6.0 vs. 2.0, p ConclusionBaricitinib combined with traditional immunosuppressants treatment was efficacious in rsJDM. Add-on therapy of baricitinib was helpful for tapering CS dose. No serious side effects were observed in this study.</p
MOESM5 of Effect of HA330 resin-directed hemoadsorption on a porcine acute respiratory distress syndrome model
Additional file 5: Fig. S3. Dynamic expressions of plasma proteins showed with hierarchical cluster analysis. The heatmap represents the log2 transformed fold change for each protein indicated. Columns represent comparisons between T0/baseline and 8 h after T0/baseline in HA and HA-sham treatment group, respectively; rows represent protein accession numbers. Red colors indicate up-regulated proteins and green colors indicate down-regulated proteins, respectively
MOESM8 of Effect of HA330 resin-directed hemoadsorption on a porcine acute respiratory distress syndrome model
Additional file 8: Table S3. BALF proteins with significantly lower expression in LPS + HA versus LPS+HA (sham)-treated pigs
MOESM11 of Effect of HA330 resin-directed hemoadsorption on a porcine acute respiratory distress syndrome model
Additional file 11: Table S6. Lung homogenate proteins with significantly higher expression in LPSÂ +Â HA versus LPSÂ +Â HA (sham)-treated pigs
The phenotype of wild-type <i>A</i>. <i>thaliana</i> treated with Fe deficiency and exogenous ethylene.
<p>(A) Transcript abundances of the <i>ACS2</i> and <i>ACS6</i> genes in wild-type <i>Arabidopsis</i> under Fe-sufficient (100 μM EDTA-Fe) and Fe-deficient (0 μM EDTA-Fe) conditions for 2 d. Bars represent means ± SE of three replicates. Different letters represent statistically different means at <i>P</i> < 0.05 (one-way ANOVA with a Duncan post-hoc test). (B) Analysis of tissue expression of the ethylene response factor <i>AtERF4</i> under Fe-sufficient and Fe-deficient conditions for 2 d. Bars represent means ± SE of three replicates. Different letters represent statistically different means at <i>P</i> < 0.05 (one-way ANOVA with a Duncan post-hoc test). (C) The phenotype of the wild type with the exogenous ethylene promoter ACC or the ethylene inhibitor AVG treated for 5 d, scale bar = 0.8 cm. (D) The H<sup>+</sup> flux of the wild-type root hair area with the exogenous ethylene promoter ACC or the ethylene inhibitor AVG treated for 5 d.</p