Immunohistochemical expression of DSP.

<p>(A): Representative immunohistochemical images of the blank control group, the Ca(OH)₂ group, and the DBM group. a: The blank control group. Pulp morphology was normal. b- f: Ca(OH)₂ group (1, 3, 7, 14, 28 days). g- k: DBM group (1, 3, 7, 14, 28 days). (B): Mean IOD value of DSP. *means significant differences.</p

Pulp Tissue Disorganization (PTD).

<p>Pulp Tissue Disorganization (PTD).</p

Immunohistochemical expression of COL I.

<p>(A): Representative immunohistochemical images of the blank control group, the Ca(OH)₂ group, and the DBM group. a: The blank control group. Pulp morphology was normal. b- f: Ca(OH)₂ group (1, 3, 7, 14, 28 days). g- k: DBM group (1, 3, 7, 14, 28 days). (B): Mean IOD value of COL I. *means significant differences.</p

Reparative Dentin Formation (RDF).

<p>Reparative Dentin Formation (RDF).</p

Representative histological images of the Ca(OH)₂ and DBM groups (x100 magnification, H&E staining).

<p>A: The blank control group. Pulp morphology was normal. B-F: Ca(OH)₂ group (1, 3, 7, 14, and 28 days). G-K: DBM group (1, 3, 7, 14, and 28 days). Inflammatory reactions began from 1 day and continued to exist through the observation period. Reparative dentin formation could be observed in both groups on 28 days, and the DBM group showed more regular and thinner reparative dentin. Arrowheads indicate inflammatory reaction. RD means reparative dentin.</p

Inflammatory Cell Infiltration (ICI).

<p>Inflammatory Cell Infiltration (ICI).</p

Results of the histopathological evaluation.

<p>ICI: Inflammatory cell infiltration, PTD: Pulp tissue disorganization, RDF: Reparative dentin formation.</p

Immunohistochemical expression of Runx2.

<p>(A): Representative immunohistochemical images of the blank control group, the Ca(OH)₂ group and the DBM group. a: The blank control group. Pulp morphology was normal. b- f: Ca(OH)₂ group (1, 3, 7, 14, 28 days). g- k: DBM group (1, 3, 7, 14, 28 days). (B): Mean IOD value of Runx2. *means significant difference.</p