Entropy generation of secondary flow in a turning passage with different boundary layer characteristics

The development of secondary flow along a curved channel is a fundamental flow phenomenon occurring in a wide range of engineering applications, including turbomachinery, aerospace, heating, ventilation, air conditioning, etc. The underlying flow physics about end-wall secondary flows has been well-documented in the open literature, while the interaction between a secondary flow and a side-wall boundary layer, which is critical to the aerothermal performance of a side-wall surface, has not been comprehensively studied. In this study, the entropy generation of secondary flow and the interaction between an end-wall passage vortex and a side-wall boundary layer were numerically investigated by Reynolds-averaged Navier–Stokes (RANS) CFD for a 90° curved channel. The transportation effect of secondary flow and the generation mechanism of an induced vortex pair on the side wall is reported. It was also found that the growth of the secondary flow can be suppressed due to the displacement effect of the side-wall boundary layer. Furthermore, it was found that the interaction between a secondary flow and a side-wall boundary layer provides a suppression effect on side-wall boundary layer separation

Cascade Regulation of the Proliferation, Recruitment, and Differentiation of Stem Cells to Prevent Aseptic Loosening by GNPs/ECPP Particles Responding to Macrophages: <i>In Vitro</i> and <i>In Vivo</i>

Modulating both inflammation and stem cells by designing an artificial joint material to obtain the continuous prevention and control on aseptic loosening (AL) is a novel strategy. In this paper, graphene/europium-doped calcium polyphosphate (GNPs/ECPP) particles were obtained by ultrasound method and spark plasma sintering (SPS) method. The prepared particles were used to modulate the inflammatory response and further obtain cascade regulation on the proliferation, recruitment, and differentiation of stem cells. The results showed that particles obtained by SPS had a stronger effect on promoting the proliferation and differentiation of stem cells, while by ultrasound method more stem cells were recruited. Besides, the graphene and Eu3+ contained in the particles obtained by SPS method could effectively play a synergistic role on the differentiation of stem cells. In vivo experiment results demonstrated that the composite particles effectively suppress the inflammatory response, recruit stem cells, and prevent AL by inhibiting the secretion of inflammatory factors

Comparison of mean scores of quality of life according to socio-demographic, clinical and disease-related characteristics.

<p>Note</p><p>*P<0.05 and</p><p>**<i>P</i><0.01</p><p>Comparison of mean scores of quality of life according to socio-demographic, clinical and disease-related characteristics.</p

Ultrathin Two-Dimensional MnO₂/Graphene Hybrid Nanostructures for High-Performance, Flexible Planar Supercapacitors

Planar supercapacitors have recently attracted much attention owing to their unique and advantageous design for 2D nanomaterials based energy storage devices. However, improving the electrochemical performance of planar supercapacitors still remains a great challenge. Here we report for the first time a novel, high-performance in-plane supercapacitor based on hybrid nanostructures of quasi-2D ultrathin MnO₂/graphene nanosheets. Specifically, the planar structures based on the δ-MnO₂ nanosheets integrated on graphene sheets not only introduce more electrochemically active surfaces for absorption/desorption of electrolyte ions, but also bring additional interfaces at the hybridized interlayer areas to facilitate charge transport during charging/discharging processes. The unique structural design for planar supercapacitors enables great performance enhancements compared to graphene-only devices, exhibiting high specific capacitances of 267 F/g at current density of 0.2 A/g and 208 F/g at 10 A/g and excellent rate capability and cycling stability with capacitance retention of 92% after 7000 charge/discharge cycles. Moreover, the high planar malleability of planar supercapacitors makes possible superior flexibility and robust cyclability, yielding capacitance retention over 90% after 1000 times of folding/unfolding. Ultrathin 2D nanomaterials represent a promising material platform to realize highly flexible planar energy storage devices as the power back-ups for stretchable/flexible electronic devices

Socio-demographic and clinical characteristics of the PLWHA on survey.

<p>Key: HIV–Human immunodeficiency virus, SD–standard deviation, HAART–highly active antiretroviral therapy</p><p>Socio-demographic and clinical characteristics of the PLWHA on survey.</p

Trimethyl chitosan–cysteine-based nanoparticles as an effective delivery system for portulacerebroside A in the management of hepatocellular carcinoma cells <i>in vitro</i> and <i>in vivo</i>

Portulacerebroside A (PCA), a cerebroside compound extracted from Portulaca oleracea L., has been shown to suppress hepatocellular carcinoma (HCC) cells. This study aims to investigate the effectiveness of trimethyl chitosan–cysteine (TMC-Cys) nanocarrier in delivering PCA for HCC management and to elucidate the molecular mechanisms behind PCA’s function. TMC-Cys nanocarriers notably augmented PCA’s function, diminishing the proliferation, migration, and invasiveness of HCC cells in vitro, reducing hepatocellular tumorigenesis in immunocompetent mice, and impeding metastasis of xenograft tumours in nude mice. Comprehensive bioinformatics analyses, incorporating Super-PRED systems alongside pathway enrichment analysis, pinpointed toll-like receptor 4 (TLR4) and epidermal growth factor receptor (EGFR) as two promising targets of PCA, enriched in immune checkpoint pathway. PCA/nanocarrier (PCA) reduced levels of TLR4 and EGFR and their downstream proteins, including programmed cell death ligand 1, thereby increasing populations and activity of T cells co-cultured with HCC cells in vitro or in primary HCC tumours in mice. However, these effects were counteracted by additional artificial activation of TLR4 and EGFR. In conclusion, this study provides novel evidence of PCA’s function in immunomodulation in addition to its direct tumour suppressive effect. TMC-Cys nanocarriers significantly enhance PCA efficacy, indicating promising application as a drug delivery system.</p

Histone H3 K27 acetylation marks a potent enhancer element on the adipogenic master regulator gene Pparg2

<div><p>PPARγ2 is expressed almost exclusively in adipose tissue and plays a central role in adipogenesis. Despite intensive studies over the last 2 decades, the mechanism regulating the expression of the Pparg2 gene, especially the role of cis-regulatory elements, is still not completely understood. Here, we report a comprehensive investigation of the enhancer elements within the murine Pparg2 gene. Utilizing the combined techniques of sequence conservation analysis and chromatin marker examination, we identified a potent enhancer element that augmented the expression of a reporter gene under the control of the Pparg2 promoter by 20-fold. This enhancer element was first identified as highly conserved non-coding sequence 10 (CNS10) and was later shown to be enriched with the enhancer marker H3 K27 acetylation. Further studies identified a binding site for p300 as the essential enhancer element in CNS10. Moreover, p300 physically binds to CNS10 and is required for the enhancer activity of CNS10. The depletion of p300 by siRNA resulted in significantly impaired activation of Pparg2 at the early stages of 3T3-L1 adipogenesis. In summary, our study identified a novel enhancer element on the murine Pparg2 gene and suggested a novel mechanism for the regulation of Pparg2 expression by p300 in 3T3-L1 adipogenesis.</p></div

Comparative Transcriptomic and Epigenomic Analyses Reveal New Regulators of Murine Brown Adipogenesis

<div><p>Increasing energy expenditure through brown adipocyte recruitment is a promising approach to combat obesity. We report here the comprehensive profiling of the epigenome and transcriptome throughout the lineage commitment and differentiation of C3H10T1/2 mesenchymal stem cell line into brown adipocytes. Through direct comparison to datasets from differentiating white adipocytes, we systematically identify stage- and lineage-specific coding genes, lncRNAs and microRNAs. Utilizing chromatin state maps, we also define stage- and lineage-specific enhancers, including super-enhancers, and their associated transcription factor binding motifs and genes. Through these analyses, we found that in brown adipocytes, brown lineage-specific genes are pre-marked by both H3K4me1 and H3K27me3, and the removal of H3K27me3 at the late stage is necessary but not sufficient to promote brown gene expression, while the pre-deposition of H3K4me1 plays an essential role in poising the brown genes for expression in mature brown cells. Moreover, we identify SOX13 as part of a p38 MAPK dependent transcriptional response mediating early brown cell lineage commitment. We also identify and subsequently validate PIM1, SIX1 and RREB1 as novel regulators promoting brown adipogenesis. Finally, we show that SIX1 binds to adipogenic and brown marker genes and interacts with C/EBPα, C/EBPβ and EBF2, suggesting their functional cooperation during adipogenesis.</p></div

Subcellular localization of KIF20A in porcine oocytes.

<p>Immunofluorescent staining was employed to show the subcellular localization of KIF20A. KIF20A exhibited a dynamic localization pattern during porcine oocyte meiotic maturation. KIF20A localized at centromeres at the germinal vesicle breakdown (GVBD) stage and metaphase I (MI) stage, then transferred to the midbody in dividing oocytes during telophase (TI), finally, KIF20A was accumulated at centromeres again at metaphase II (MII). Red, KIF20A; blue, chromatin.</p

Effects of KIF20A inhibition on spindle structure and chromosome alignment of porcine oocytes.

<p>(A) Normal Spindle structure and chromosome alignment were investigated after KIF20A inhibition. (<b>B</b>) Rates of abnormal oocytes (displaying chromosome misalignment and aberrant spindles) after KIF20A inhibition.</p