Methods and compositions for transposition using minimal segments of the eukaryotic transformation vector Piggybac

The present invention provides efficient transfer of genes into host cells or embryos to transform the cells or embryos by transposition vectors using the minimal amount of nucleotide sequences in the transposon piggyBac required for gene transfer. The transformed cells or embryos may also be developed into transgenic organisms

Methods and compositions for transposition using minimal segments of the eukaryotic transformation vector piggyBac

More efficient transfer of genes into host cells or embryos to transform the cells or embryos is facilitated by transposition vectors using the minimal amount of nucleotide sequences in the transposon piggyBac required for gene transfer. The transformed cells or embryos may be developed into transgenic organisms

Overall model fit indices for different number of latent classes and latent class membership probabilities for the three-class solution.

Overall model fit indices for different number of latent classes and latent class membership probabilities for the three-class solution.</p

Descriptive statistics and bivariate correlations of study variables.

Descriptive statistics and bivariate correlations of study variables.</p

Coping online with Racism Scale.

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Description of the final three-class solution: Means of and associations between indicator variables.

Description of the final three-class solution: Means of and associations between indicator variables.</p

Prediction of latent class membership by racial socialization dimensions and health indicators.

Prediction of latent class membership by racial socialization dimensions and health indicators.</p

Cross-table of race by gender groups and three latent classes.

Cross-table of race by gender groups and three latent classes.</p

Preparation and Characterization of Inclusion Complexes of Biodegradable Amphiphilic Poly(ethylene oxide)−Poly[(<i>R</i>)-3-hydroxybutyrate]−Poly(ethylene oxide) Triblock Copolymers with Cyclodextrins

Inclusion complexes (ICs) of biodegradable amphiphilic poly(ethylene oxide)−poly[(R)-3-hydroxybutyrate]−poly(ethylene oxide) triblock copolymers with α-cyclodextrin (α-CD) or γ-cyclodextrin (γ-CD) were prepared from aqueous medium. The ICs were characterized by XRD, DSC, 13C CP/MAS NMR, 1H NMR, FTIR, and TGA. The results of XRD and 13C CP/MAS NMR indicated that the ICs formed channel structure, and the α-CD or γ-CD molecules in the ICs adopted a more symmetric conformation compared with their uncomplexed states. As to the triblock copolymers, while the poly(ethylene oxide) blocks were included in the channel structure, the poly[(R)-3-hydroxybutyrate] block was partially threaded by α-CD or γ-CD, which was confirmed by the results of XRD, DSC, 1H NMR, and FTIR. The formation of ICs led to an increase in the thermal stability of both cyclodextrins and the triblock copolymers