Data_Sheet_1_Different Responses of Soil Bacterial Communities to Nitrogen Addition in Moss Crust.zip

Bacterial communities in soil serve an important role in controlling terrestrial biogeochemical cycles and ecosystem processes. Increased nitrogen (N) deposition in Northwest China is generating quantifiable changes in many elements of the desert environment, but the impacts of N deposition, as well as seasonal variations, on soil bacterial community composition and structure are poorly understood. We used high-throughput sequencing of bacterial 16S rRNA genes from Gurbantünggüt Desert moss crust soils to study the impacts of N addition on soil bacterial communities in March, May, and November. In November, we discovered that the OTU richness and diversity of soil bacterial community dropped linearly with increasing N input. In November and March, the diversity of the soil bacterial community decreased significantly in the medium-N and high-N treatments. In May, N addition caused a substantial change in the makeup of the soil bacterial composition, while the impacts were far less apparent in November and March. Furthermore, the relative abundance of major bacterial phyla reacted non-linearly to N addition, with high-N additions decreasing the relative richness of Proteobacteria, Bacteroidetes, and Acidobacteria while increasing the relative abundance of Actinobacteria and Chloroflexi. We also discovered that seasonality, as characterized by changes in soil moisture, pH, SOC, and AK content, had a significant impact on soil bacterial communities. Significant variations in the makeup of the community were discovered at the phylum and genus levels throughout the various months. In May, the variety of soil bacterial community was at its peak. Further investigation showed that the decrease in soil bacterial diversity was mostly attributed to a drop in soil pH. These results indicated that the impact of N deposition on the soil bacterial community was seasonally dependent, suggesting that future research should evaluate more than one sample season at the same time.</p

Additional file 12 of Codon usage pattern of the ancestor of green plants revealed through Rhodophyta

Additional file 12: Supplementary Figure S1. The plotting for GC3s of CDSs and GC content of introns among eight species. The correlation between two statistical data points is represented by the plotting of the GC3s of CDSs and GC content of introns in the same gene. The black solid line represents the correlation line, and its equation is shown at the top of the plot

Additional file 11 of Codon usage pattern of the ancestor of green plants revealed through Rhodophyta

Additional file 11: Supplementary Table S11. Versions of genomes and annotation files and taxonomic information for the eight red algae

Additional file 2 of Codon usage pattern of the ancestor of green plants revealed through Rhodophyta

Additional file 2: Supplementary Table S2. Correlation analysis and t test (double tail) for the GC3s of CDSs and GC content of introns among the eight red algae

Additional file 1 of Codon usage pattern of the ancestor of green plants revealed through Rhodophyta

Additional file 1: Supplementary Table S1. GC3s of CDSs, GC content of flanking sequences and introns respectively in the three bias categories from the eight red algae

Additional file 6 of Codon usage pattern of the ancestor of green plants revealed through Rhodophyta

Additional file 6: Supplementary Table S6. Number of tRNAs and anticodons of different amino acids in the eight red algae (S6a-S6h)

Additional file 8 of Codon usage pattern of the ancestor of green plants revealed through Rhodophyta

Additional file 8: Supplementary Table S8. The average Fop values for the domain and nondomain codons of the three bias gene categories in the eight red algae

Additional file 5 of Codon usage pattern of the ancestor of green plants revealed through Rhodophyta

Additional file 5: Supplementary Table S5. The optimal codon which lack perfectly matching complementary tRNA genes in multicellular red algae

Additional file 3 of Codon usage pattern of the ancestor of green plants revealed through Rhodophyta

Additional file 3: Supplementary Table S3. Output of optimal codon analysis from Codon W in the eight red algae (S3a-S3h)

Additional file 9 of Codon usage pattern of the ancestor of green plants revealed through Rhodophyta

Additional file 9: Supplementary Table S9. Odds ratios and their logarithmic values(Ln) of two degenerate amino acids among the eight red algae