Additional file 4: of Genomic variations and association study of agronomic traits in flax

Table S2. Predicted genes in the candidate regions by GWAS using GLM model. (DOCX 15 kb

Additional file 7: of Genomic variations and association study of agronomic traits in flax

Table S3. The information of 224 flax accessions. (XLSX 16 kb

Additional file 1: of Genomic variations and association study of agronomic traits in flax

Figure S1. LD decay of 224 flax accessions. (PNG 40 kb

Additional file 3: of Genomic variations and association study of agronomic traits in flax

Figure S2. Putative metabolic pathway associated with palmitic acid content. (PNG 29 kb

Additional file 2: of Genomic variations and association study of agronomic traits in flax

Table S1. Predicted genes in the candidate regions by GWAS using EMMAX model. (DOCX 21 kb

Additional file 6: of Genomic variations and association study of agronomic traits in flax

Figure S3. Neutrality tests. (TIF 1802 kb

Identification and Analysis of Flax Resistance Genes to <i>Septoria linicola</i> (Speg.) Garassini

In this study, the flax (Linum usitatissimum L.) genes associated with resistance to the disease pasmo (Septoria linicola (Speg.) Garassini) were mapped using genome resequencing and bulked segregant analysis (BSA) of genomic DNA of the pasmo-resistant parent y62–9, pasmo-susceptible parent y64–5, and F2 generation segregants. Pasmo resistance genes were identified using Gene Ontology (GO) functional prediction and gene annotation methods. A Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis revealed the biological information and molecular mechanisms associated with the flax-pathogen interaction. The results of a quantitative real-time PCR analysis revealed that the levels of expression of the three genes Lus10003106, Lus10022077, and Lus10021999 differed between the y62–9 (pasmo-resistant) and y64–5 (pasmo-susceptible) parental flax lines after the inoculation of plants with the pasmo pathogen. Thus, these genes may play key roles in the resistance of flax to pasmo. The results of this study provide a foundation to support future studies of the pathogenesis of flax disease and the discovery and cloning of resistance genes and development of new molecular markers toward the development of pasmo-resistant flax varieties.</p