Effects of different salt treatments on dry weight accumulation and allocation of <i>Elaeagnus angustifolia</i> seedlings.

<p>Effects of different salt treatments on dry weight accumulation and allocation of <i>Elaeagnus angustifolia</i> seedlings.</p

It's Christmas Carol (2006)

1. Poster, 2. Photo, 3. Photo, 4. Photo, 5. Photo, 6. Photo, 7. Photo, 8. Photo, 9. Photo, 10. Photo, 11. Photo, 12. Photo, 13. Photo, 14. Photo, 15. Program, 16. Press Release English, 17. Press Release FrenchArchival file for the Glendon College production of It's Christmas Carol, written and directed by Nicole Toogood. The play was performed November 30th - December 9th, 2006

Ranking of candidate reference genes in order of their expression stability as calculated by NormFinder.

<p>Note: Expression stability and ranking of 10 reference genes as calculated by NormFinder in all samples (A), different ages (B), different tissue types (C), cold-treated (D), heat-treated (E), NaCl-treated (F), PEG-treated (G), ABA-treated (H). Lower average expression stability (M value) indicates more stable expression.</p

Descriptions of candidate genes from <i>Platycladus orientalis</i> for qRT-PCR.

<p>Note: All reference gene sequences from transcriptome data of <i>Platycladus orientalis</i> were searched with BLAST using sequences of <i>Arabidopsis thaliana</i> in GenBank. Sequences of candidate housekeeping genes and NAC domain protein gene are provided in the Supporting Information.</p

Expression profiles of <i>NAC</i> in different-aged tissues and in response to stresses in <i>Platycladus orientalis</i> (as determined by qRT-PCR with UBC and aTUB in combination as reference genes).

<p>Expression profiles of <i>NAC</i> in different-aged tissues and in response to stresses in <i>Platycladus orientalis</i> (as determined by qRT-PCR with UBC and aTUB in combination as reference genes).</p

Additional file 2 of Integrated metabolome and transcriptome analysis unveils novel pathway involved in the fruit coloration of Nitraria tangutorum Bobr.

Additional file 2: Supplementary Table 1. The raw data for the detected metabolic species in our manuscript. Supplementary Table 2. Differential metabolic species among the JC and NT. Supplementary Table 3. Summary of transcriptome sequencing data transcriptome assembly. Supplementary Table 4. Details of all the gene annotation. Supplementary Table 5. KOG function annotation of all unigenes. Supplementary Table 6. KEGG pathway annotation of all unigenes. Supplementary Table 7. Details of all the different expression genes in JC and NT.  Supplementary Table 8. KEGG pathways in which differentially expressed genes are involved. Supplementary Table 9. All DEGs of anthocyanin biosynthesis pathway. The different expression coding genes of key enzyme anthocyanin biosynthesis pathway after WGCNA. Supplementary Table 10. Statistical table of TFs in JC and NT. Supplementary Table 11. Primers used in qRT-PCR in this study

Rational Design and Structure Validation of a Novel Peptide Inhibitor of the Adenomatous-Polyposis-Coli (APC)–Rho-Guanine-Nucleotide-Exchange-Factor‑4 (Asef) Interaction

In colorectal cancer, adenomatous polyposis coli (APC) interacts with Rho guanine-nucleotide-exchange factor 4 (Asef), thereby stimulating aberrant colorectal-cancer-cell migration. Consequently, the APC–Asef interaction represents a promising therapeutic target for mitigating colorectal-cancer migration. In this study, we adopted the rational-design strategy involving the introduction of intramolecular hydrogen bonds and optimization of the lipophilic substituents to improve the binding affinities of peptides, leading to the discovery of MAI-400, the best inhibitor of the APC–Asef interaction known to date (Kd = 0.012 μM, IC50 = 0.25 μM). Comprehensive evaluation of MAI-400 by biochemical and biophysical assays revealed the formation and effect of an intramolecular hydrogen bond. A cell-based assay showed MAI-400 efficiently blocking the APC–Asef interaction in a dose-dependent manner. Therefore, our study provides a best-in-class inhibitor, MAI-400, based on the rational drug design and structural validation, that can effectively inhibit the APC–Asef interaction

Figure 1

<p> <b>Expression levels of candidate reference genes in different plant samples.</b></p

Determination of the optimal number of reference genes for normalization by pairwise variation (V) using geNorm.

<p>Determination of the optimal number of reference genes for normalization by pairwise variation (V) using geNorm.</p

Gene expression stability and ranking of 10 reference genes as calculated by geNorm.

<p>Gene expression stability and ranking of 10 reference genes as calculated by geNorm.</p