3 research outputs found

    Atomically Precise Au<sub>25</sub>(SG)<sub>18</sub> Nanoclusters: Rapid Single-Step Synthesis and Application in Photothermal Therapy

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    Remarkable recent advances on Au<sub>25</sub>(SR)<sub>18</sub> nanoclusters have led to significant applications in catalysis, sensing, and magnetism. However, the existing synthetic routes are complicated, particularly for the water-soluble Au<sub>25</sub>(SG)<sub>18</sub> nanoclusters. Here, we report a single-step concentration and temperature-controlled method for rapid synthesis of the Au<sub>25</sub>(SG)<sub>18</sub> nanoclusters in as little as 2 h without the need for low-temperature reaction or even stirring. A systematic time-based investigation was carried out to study the effects of volume, concentration, and temperature on the synthesis of these nanoclusters. Further, we discovered for the first time that the Au<sub>25</sub>(SG)<sub>18</sub> nanoclusters exhibit excellent photothermal activities in achieving 100% cell death for MDA-MB-231 breast cancer cells at a power of 10 W/cm<sup>2</sup> using an 808 nm laser source, demonstrating applications toward photothermal therapy

    Exploration of Nanoparticle-Mediated Photothermal Effect of TMB‑H<sub>2</sub>O<sub>2</sub> Colorimetric System and Its Application in a Visual Quantitative Photothermal Immunoassay

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    The exploration of new physical and chemical properties of materials and their innovative application in different fields are of great importance to advance analytical chemistry, material science, and other important fields. Herein, we, for the first time, discovered the photothermal effect of an iron oxide nanoparticles (NPs)-mediated TMB (3,3′,5,5′-tetramethylbenzidine)-H<sub>2</sub>O<sub>2</sub> colorimetric system, and applied it toward the development of a new NP-mediated photothermal immunoassay platform for visual quantitative biomolecule detection using a thermometer as the signal reader. Using a sandwich-type proof-of-concept immunoassay, we found that the charge transfer complex of the iron oxide NPs-mediated one-electron oxidation product of TMB (oxidized TMB) exhibited not only color changes, but also a strong near-infrared (NIR) laser-driven photothermal effect. Hence, oxidized TMB was explored as a new sensitive photothermal probe to convert the immunoassay signal into heat through the near-infrared laser-driven photothermal effect, enabling simple photothermal immunoassay using a thermometer. Based on the new iron oxide NPs-mediated TMB-H<sub>2</sub>O<sub>2</sub> photothermal immunoassay platform, prostate-specific antigen (PSA) as a model biomarker can be detected at a concentration as low as 1.0 ng·mL<sup>–1</sup> in normal human serum. The discovered photothermal effect of the colorimetric system and the developed new photothermal immunoassay platform open up a new horizon for affordable detection of disease biomarkers and have great potential for other important material and biomedical applications of interest

    Capillary blood for point-of-care testing

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    <p>Clinically, blood sample analysis has been widely used for health monitoring. In hospitals, arterial and venous blood are utilized to detect various disease biomarkers. However, collection methods are invasive, painful, may result in injury and contamination, and skilled workers are required, making these methods unsuitable for use in a resource-limited setting. In contrast, capillary blood is easily collected by a minimally invasive procedure and has excellent potential for use in point-of-care (POC) health monitoring. In this review, we first discuss the differences among arterial blood, venous blood, and capillary blood in terms of the puncture sites, components, sample volume, collection methods, and application areas. Additionally, we review the most recent advances in capillary blood-based commercial products and microfluidic instruments for various applications. We also compare the accuracy of microfluidic-based testing with that of laboratory-based testing for capillary blood-based disease diagnosis at the POC. Finally, we discuss the challenges and future perspectives for developing capillary blood-based POC instruments.</p
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