Image_3_Successful treatment of a pure red-cell aplasia patient with γδT cells and clonal TCR gene rearrangement: A case report.jpeg

Pure red-cell aplasia (PRCA) is a syndrome associated with reduced erythroid precursors. This report presents the case of an elderly PRCA patient with significantly proliferated γδT cells and clonal T-cell receptor (TCR) gene rearrangement. The cause of this patient’s PRCA was confirmed to be an autoimmune disorder rather than malignancy on the basis of flow cytometry, TCR gene rearrangement, and positron emission tomography/computed tomography (PET/CT) findings. Moreover, the γδT cell group identified in this case was captured for the first time under the microscope; this CD4+/CD8− (extremely high CD4/CD8 ratio) population is rare in PRCA patients. Our patient with a monoclonal and polyclonal hybrid of TCR gene rearrangement was sensitive to cyclosporin A (CsA), despite previous reports suggesting that patients with TCR clonal rearrangement may respond poorly to this drug. Overall, this case presents valuable clinical findings for the future diagnosis and management of PRCA caused by autoimmune conditions and further research on γδT cells’ autoimmune pathophysiology and gene rearrangement.</p

Supplementary document for Adaptive real-time single-pixel imaging - 6838594.pdf

This document includes the programming details of the proposed method and the illustration of how each of the pattern sequences is arranged to achieve adaptive real-time single-pixel imaging

Image_1_Successful treatment of a pure red-cell aplasia patient with γδT cells and clonal TCR gene rearrangement: A case report.jpeg

Pure red-cell aplasia (PRCA) is a syndrome associated with reduced erythroid precursors. This report presents the case of an elderly PRCA patient with significantly proliferated γδT cells and clonal T-cell receptor (TCR) gene rearrangement. The cause of this patient’s PRCA was confirmed to be an autoimmune disorder rather than malignancy on the basis of flow cytometry, TCR gene rearrangement, and positron emission tomography/computed tomography (PET/CT) findings. Moreover, the γδT cell group identified in this case was captured for the first time under the microscope; this CD4+/CD8− (extremely high CD4/CD8 ratio) population is rare in PRCA patients. Our patient with a monoclonal and polyclonal hybrid of TCR gene rearrangement was sensitive to cyclosporin A (CsA), despite previous reports suggesting that patients with TCR clonal rearrangement may respond poorly to this drug. Overall, this case presents valuable clinical findings for the future diagnosis and management of PRCA caused by autoimmune conditions and further research on γδT cells’ autoimmune pathophysiology and gene rearrangement.</p

Image_2_Successful treatment of a pure red-cell aplasia patient with γδT cells and clonal TCR gene rearrangement: A case report.jpeg

Pure red-cell aplasia (PRCA) is a syndrome associated with reduced erythroid precursors. This report presents the case of an elderly PRCA patient with significantly proliferated γδT cells and clonal T-cell receptor (TCR) gene rearrangement. The cause of this patient’s PRCA was confirmed to be an autoimmune disorder rather than malignancy on the basis of flow cytometry, TCR gene rearrangement, and positron emission tomography/computed tomography (PET/CT) findings. Moreover, the γδT cell group identified in this case was captured for the first time under the microscope; this CD4+/CD8− (extremely high CD4/CD8 ratio) population is rare in PRCA patients. Our patient with a monoclonal and polyclonal hybrid of TCR gene rearrangement was sensitive to cyclosporin A (CsA), despite previous reports suggesting that patients with TCR clonal rearrangement may respond poorly to this drug. Overall, this case presents valuable clinical findings for the future diagnosis and management of PRCA caused by autoimmune conditions and further research on γδT cells’ autoimmune pathophysiology and gene rearrangement.</p

Data_Sheet_1_Development and validation of Child-Friendly School Environment Questionnaire from Chinese culture.pdf

In the context of building Child-Friendly Cities in China, child-friendly school environments are considered as having a profound impact on children’s development and growth. This study presents the development and validation of the Child-Friendly School Environment Questionnaire for assessing a child-friendly school environment. Utilizing open-ended questions and interviews, an initial questionnaire on the child-friendly school environment was compiled. An exploratory factor analysis of the preliminary test results with 696 primary school children in grades three to six was conducted to refine the questionnaire into a formal 19-item questionnaire. Subsequently, a confirmatory factor analysis was performed to analyze the evaluation results of 807 primary school children in grades three to six. The results indicated that a child-friendly school environment is a multi-dimensional construct encompassing Environment Friendly, Teaching Friendly, Peer Friendly, and Children Participation, with good reliability and validity. The promising outcomes of this study suggest that the Child-Friendly School Environment Questionnaire can be widely used as a powerful evaluation tool for the child-friendly school education practice in the future.</p

Destabilization of Human Islet Amyloid Polypeptide Fibrils by Charged Graphene Quantum Dots: A Molecular Dynamics Investigation with Implications for Nanomedicine

Human islet amyloid polypeptide (hIAPP) is the major component of the amyloid deposited in the pancreas of patients with type 2 diabetes mellitus. Its aggregation and consequent production of intermediates are believed to be responsible for its cytotoxicity and pathological processes. Recently, graphene quantum dots (GQDs) are proved to effectively inhibit a range of amyloid deposits. This work focuses on the influence of the charged GQDs on hIAPP inhibition. Microsecond all-atom molecular dynamics simulations in explicit water were performed to study the influence of charged GQDs on the structural stability of hIAPP fibril. GQDs were found to be able to destabilize the hIAPP fibril and reduce the β-sheet content. The stability of the hydrophobic core was greatly disturbed, and the hydrogen bond formation at protofibril interfaces was also hindered. The negatively and positively charged GQDs have different binding sites, dynamics, and interactions at hIAPP fibril, which is dominated by electrostatic interaction and assisted by π–π stacking, salt bridge, and hydrogen bonding interactions. The π–π stacking between GQDs and hIAPP may be influenced by the electrostatic interaction in a facilitative or competitive manner. In addition, the negatively charged GQD is suggested to be a better candidate of amyloid inhibition than the positively charged one in disruptive effect, binding modes, and binding intensity. These findings may provide useful perspectives for the design of nanomedicine for amyloid inhibition and are helpful to the development of diagnosis and screening nanotechnology for neurodegenerative diseases

Table_1_v1_A case report of primary colonic paraganglioma with lymph node metastasis.docx

BackgroundParaganglioma is a kind of neuroendocrine tumor that originates from paraganglia outside the adrenal gland. Gastrointestinal tract paraganglioma is very rare and only four cases of paraganglioma originating in the colon have been reported.Case PresentationWe report a case of metastatic paraganglioma originating in the colon, in which the differential diagnosis was established by comprehensively considering clinical information, histology, immunohistochemistry, and findings of fluorescence in situ hybridization and next generation sequencing analyses. The patient has remained well for over 14 months after the treatment.ConclusionSince all paraganglioma have metastatic potential, we believe that radical surgical resection and regular follow-up are necessary. Genetic testing may be indicative of metastatic potential and prognosis. Because colonic paraganglioma is very rare, differential diagnosis is very important. Our report provides experience for the diagnosis and study of paraganglioma in rare sites.</p

Table1_Deep Learning-Based Recognition of Different Thyroid Cancer Categories Using Whole Frozen-Slide Images.DOCX

Introduction: The pathological rare category of thyroid is a type of lesion with a low incidence rate and is easily misdiagnosed in clinical practice, which directly affects a patient’s treatment decision. However, it has not been adequately investigated to recognize the rare, benign, and malignant categories of thyroid using the deep learning method and recommend the rare to pathologists.Methods: We present an empirical decision tree based on the binary classification results of the patch-based UNet model to predict rare categories and recommend annotated lesion areas to be rereviewed by pathologists.Results: Applying this framework to 1,374 whole-slide images (WSIs) of frozen sections from thyroid lesions, we obtained an area under a curve of 0.946 and 0.986 for the test datasets with and without WSIs, respectively, of rare types. However, the recognition error rate for the rare categories was significantly higher than that for the benign and malignant categories (p Conclusion: Our study provides a baseline for the recommendation of the uncertain predicted rare category to pathologists, offering potential feasibility for the improvement of pathologists’ work efficiency.</p

Binary Solvent Engineering for High-Performance Two-Dimensional Perovskite Solar Cells

Two-dimensional (2D) organic–inorganic hybrid perovskite materials have recently attracted tremendous attention for use in perovskite solar cells (PSCs) by virtue of their considerable long-term stability. However, the primary stumbling block for their application in PSC is their relatively lower power conversion efficiency (PCE) compared with the conventional 3D perovskite. In this work, through a binary solvent engineering of dimethylformamide (DMF)/dimethyl sulfoxide (DMSO) in the precursor solution, high-performance 2D perovskite planar solar cells are fabricated with beyond 10% PCE and outstanding environmental stability. In the formation of the 2D perovskite film during the hot-coating process, heterogeneous nucleation primarily occurs at the air–liquid interface, contributing to the formation of the thin, flaky cap layer on the outmost surface of the film. Sequentially, for the unary solvent DMF, its fast volatilization facilitates homogeneous nucleation, resulting in random-orientated perovskite grains in the inner layer. In contrast, with the binary solvent engineering of DMF/DMSO, the formation of intermediate can slow the crystallization process, making the perovskite cap layer serve as the seed that impels the subsequent perovskite crystallization to be vertically orientated in the inner layer of the perovskite film. These findings provide not only a new understanding of the 2D perovskite crystallization process but also a simple and effective method to fabricate high-performance 2D perovskite-based photovoltaics

Additional file 1: of Smaller gray matter volume of hippocampus/parahippocampus in elderly people with subthreshold depression: a cross-sectional study

STROBE Statement—Checklist of items that should be included for cross-sectional studies (XLSX 14 kb