Figures S1 - S5 from Preparation of core–shell structured CaCO₃ microspheres as rapid and recyclable adsorbent for anionic dyes

N₂ adsorption/desorption isotherms and pore-size distribution (inset) of the obtained core-shell structured CaCO₃ MSs; TG analysis of Hesp (a), pure CaCO₃ without Hesp and the obtained core-shell structured CaCO₃ MSs (b).; SEM image of the CaCO₃ MSs after 5 recycling experiments.; N₂ adsorption/desorption isotherms and pore-size distribution (inset) of the CaCO₃ MSs after 5 recycling experiments.; Three-dimensional histogram of the dye adsorption efficiency on the CaCO₃ MSs in 6-10th cycles of the adsorption-desorption

Genomic Analysis of a Mycobacterium Bovis Bacillus Calmette-Guérin Strain Isolated from an Adult Patient with Pulmonary Tuberculosis

<div><p>For years, bacillus Calmette-Guérin (BCG) has served as the unique vaccine against tuberculosis and has generally been regarded as safe. However, a clinical strain labeled 3281 that was isolated from a TB patient was identified to be BCG. Via the combination of next-generation sequencing (NGS) and comparative genomic analysis, unique 3281 genetic characteristics were revealed. A region containing the dnaA and dnaN genes that is closely related to the initial chromosome replication was found to repeat three times on the BCG Pasteur-specific tandem duplication region DU1. Due to the minimum number of epitopes in BCG strains, 3281 was inferred to have a high possibility for immune evasion. Additionally, variations in the virulence genes and predictions for potential virulence factors were analyzed. Overall, we report a pathogen that has never previously been thought to be pathogenic and initial insights that are focused on the genetic characteristics of virulent BCG.</p></div

Circular representation of the <i>M</i>. <i>bovis</i> BCG 3281 chromosome.

<p>The outer black circle shows the coordinate. Moving inward, the next two circles show forward and reverse strand CDS, respectively, with colors representing the functional classification, the next circle shows RD(red) and DU (orange), followed by the 3281 unique SNP with nonsynonymous blue and synonymous red, then is the tRNA (blue) and rRNA (purple), final two are GC-content and GC-skew by using a 10-kb window.</p

Scheme showing the DU1 region of BCG 3281 and BCG Pasteur 1173p2.

<p>(A). The color schemes means duplicated regions. (B). Details of genes involved in the BCG 3281 duplicated units (using H37Rv coordinate).</p

Phylogenetic tree of <i>M</i>. <i>tuberculosis</i>, <i>M</i>. <i>Bovis</i> and BCGS.

<p>The tree was constructed employing Neighbor-joining method. It is based on the SNPs within 2263 core genes of the strains.</p

Summary of DU1 regions within <i>M</i>. <i>bovis</i> BCG Pasteur, Mexico, Tokyo, Korea and 3281.

<p>Summary of DU1 regions within <i>M</i>. <i>bovis</i> BCG Pasteur, Mexico, Tokyo, Korea and 3281.</p

Genes in the duplication unit that located at DU1 region of <i>M</i>. <i>bovis</i> BCG 3281.

<p>Genes in the duplication unit that located at DU1 region of <i>M</i>. <i>bovis</i> BCG 3281.</p

CDSs inferred with potential virulence in <i>M. bovis</i> BCG 3281.

<p>CDSs inferred with potential virulence in <i>M. bovis</i> BCG 3281.</p

Genome messages of strains used in this paper.

<p>Genome messages of strains used in this paper.</p

Comparison of mutative virulence factors within <i>M</i>. <i>bovis</i> AF2122 and <i>M</i>. <i>bovis</i> BCG 3281, Pasteur, Tokyo, Mexico and Korea.

<p>Comparison of mutative virulence factors within <i>M</i>. <i>bovis</i> AF2122 and <i>M</i>. <i>bovis</i> BCG 3281, Pasteur, Tokyo, Mexico and Korea.</p