16 research outputs found
Quantitative Proteome of Medulla Oblongata in Spontaneously Hypertensive Rats
We performed an extensive quantitative proteomic analysis
on the
pooled medulla sample of the 11-week-old spontaneously hypertensive
rats (SHR) compared to age-matched normotensive Wistar rats, using
iTRAQ technology coupled with nano two-dimentional liquid chromatography
followed by high resolution mass spectrometric abundance indexes techniques.
Many differentially expressed proteins identified were involved in
energy metabolism, such as mitochondrial part, pyruvate dehydrogenase
complex, and respiratory chain. These proteins were included in citrate
cycle (TCA cycle), pyruvate metabolism and oxidative phosphorylation.
The proteomic analysis and subsequent Western blotting on two independent
cohorts of animials indicated that the dysregulation of energy metabolism
existed in the medulla of the SHR rats. The differentially expressed
proteins in the dysregulation of energy metabolism in the medulla
of SHR rats included down-regulated ATP6V1D, ATP6VOA1, ATP5L, DLD
proteins and up-regulated AK1 protein. MAO-A protein also exhibited
decreased regulation, as well as the other 3 above-mentioned energy-relative
proteins (ATP6V1D, ATP5L and DLD proteins) belonging to the heterocycle
metabolic process. A receiver operating characteristic curve (ROC)
analysis on 4 of the differentially expressed proteins respectively
resulted in an area under the receiver operating characteristic curve
(AUC) of 0.95, 0.90, 0.92, and 0.81 for differentiating the SHR rats
from the normotensive rats. This dysfunction in energy metabolism
localizes to the medulla, the lower part of brain stem, and is, therefore,
likely to contribute to the development, as well as to pathophysiological
complications of hypertension
Quantitative Proteome of Medulla Oblongata in Spontaneously Hypertensive Rats
We performed an extensive quantitative proteomic analysis
on the
pooled medulla sample of the 11-week-old spontaneously hypertensive
rats (SHR) compared to age-matched normotensive Wistar rats, using
iTRAQ technology coupled with nano two-dimentional liquid chromatography
followed by high resolution mass spectrometric abundance indexes techniques.
Many differentially expressed proteins identified were involved in
energy metabolism, such as mitochondrial part, pyruvate dehydrogenase
complex, and respiratory chain. These proteins were included in citrate
cycle (TCA cycle), pyruvate metabolism and oxidative phosphorylation.
The proteomic analysis and subsequent Western blotting on two independent
cohorts of animials indicated that the dysregulation of energy metabolism
existed in the medulla of the SHR rats. The differentially expressed
proteins in the dysregulation of energy metabolism in the medulla
of SHR rats included down-regulated ATP6V1D, ATP6VOA1, ATP5L, DLD
proteins and up-regulated AK1 protein. MAO-A protein also exhibited
decreased regulation, as well as the other 3 above-mentioned energy-relative
proteins (ATP6V1D, ATP5L and DLD proteins) belonging to the heterocycle
metabolic process. A receiver operating characteristic curve (ROC)
analysis on 4 of the differentially expressed proteins respectively
resulted in an area under the receiver operating characteristic curve
(AUC) of 0.95, 0.90, 0.92, and 0.81 for differentiating the SHR rats
from the normotensive rats. This dysfunction in energy metabolism
localizes to the medulla, the lower part of brain stem, and is, therefore,
likely to contribute to the development, as well as to pathophysiological
complications of hypertension
Baseline data.
<p>The P values with “*”were obtained using Fisher’s Exact Test, whereas the rest were the result of independent samples t-test.</p
Localization of the acupuncture specific effects by comparing TE5 verum acupuncture (Group A) vs. TE5 sham acupuncture (Group B) and TE5 verum acupuncture (Group A) vs. nonacupoint acupuncture (Group C).
<p>Abbreviation: BA, Brodmann area; Vox, voxel (represents the number of voxels); N/A, not available (means that the peak voxel was out of the BA zone). The BA area marked by “*”was corrected by a neurological physician.</p
Differences of seed associated networks between ROIs from the left hemisphere and the right hemisphere.
<p>Full line represents stronger correlation under acupuncture compared with sham acupuncture, whereas the “dash–dot–dot” line represents weaker correlation. Dash line stands for weaker correlation compared with nonacupoint acupuncture (P<0.05, multiple comparison error corrected using Monte Carlo simulation). Regions of the left hemisphere and right hemisphere that had significant differences in correlation with seeds are placed on left side and right side, respectively, and ROIs in the same box are from the same hemisphere.</p
The expression of protein spots in the medulla of four different groups of rats.
*<p><i>P</i><0.01, model group vs. Taichong point group or normal group;</p>#<p><i>P</i><0.01, Taichong group vs. non-acupoint group;</p>â–ľ<p><i>P</i><0.01, Taichong group vs. normal group;</p>â–´<p><i>P</i><0.01, non-acupoint group vs. model group; and <sup>â–Ş</sup><i>P</i><0.01, non-acupoint group vs. normal group.</p
Differentially expressed proteins among 4 groups of rats.
<p>Differentially expressed proteins among 4 groups of rats.</p
Relative protein expression among four different groups of rats.
<p>
<b>↑: up-regulated; ↓: down-regulated; Τ: unchanged.</b></p