16 research outputs found

    Quantitative Proteome of Medulla Oblongata in Spontaneously Hypertensive Rats

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    We performed an extensive quantitative proteomic analysis on the pooled medulla sample of the 11-week-old spontaneously hypertensive rats (SHR) compared to age-matched normotensive Wistar rats, using iTRAQ technology coupled with nano two-dimentional liquid chromatography followed by high resolution mass spectrometric abundance indexes techniques. Many differentially expressed proteins identified were involved in energy metabolism, such as mitochondrial part, pyruvate dehydrogenase complex, and respiratory chain. These proteins were included in citrate cycle (TCA cycle), pyruvate metabolism and oxidative phosphorylation. The proteomic analysis and subsequent Western blotting on two independent cohorts of animials indicated that the dysregulation of energy metabolism existed in the medulla of the SHR rats. The differentially expressed proteins in the dysregulation of energy metabolism in the medulla of SHR rats included down-regulated ATP6V1D, ATP6VOA1, ATP5L, DLD proteins and up-regulated AK1 protein. MAO-A protein also exhibited decreased regulation, as well as the other 3 above-mentioned energy-relative proteins (ATP6V1D, ATP5L and DLD proteins) belonging to the heterocycle metabolic process. A receiver operating characteristic curve (ROC) analysis on 4 of the differentially expressed proteins respectively resulted in an area under the receiver operating characteristic curve (AUC) of 0.95, 0.90, 0.92, and 0.81 for differentiating the SHR rats from the normotensive rats. This dysfunction in energy metabolism localizes to the medulla, the lower part of brain stem, and is, therefore, likely to contribute to the development, as well as to pathophysiological complications of hypertension

    Quantitative Proteome of Medulla Oblongata in Spontaneously Hypertensive Rats

    No full text
    We performed an extensive quantitative proteomic analysis on the pooled medulla sample of the 11-week-old spontaneously hypertensive rats (SHR) compared to age-matched normotensive Wistar rats, using iTRAQ technology coupled with nano two-dimentional liquid chromatography followed by high resolution mass spectrometric abundance indexes techniques. Many differentially expressed proteins identified were involved in energy metabolism, such as mitochondrial part, pyruvate dehydrogenase complex, and respiratory chain. These proteins were included in citrate cycle (TCA cycle), pyruvate metabolism and oxidative phosphorylation. The proteomic analysis and subsequent Western blotting on two independent cohorts of animials indicated that the dysregulation of energy metabolism existed in the medulla of the SHR rats. The differentially expressed proteins in the dysregulation of energy metabolism in the medulla of SHR rats included down-regulated ATP6V1D, ATP6VOA1, ATP5L, DLD proteins and up-regulated AK1 protein. MAO-A protein also exhibited decreased regulation, as well as the other 3 above-mentioned energy-relative proteins (ATP6V1D, ATP5L and DLD proteins) belonging to the heterocycle metabolic process. A receiver operating characteristic curve (ROC) analysis on 4 of the differentially expressed proteins respectively resulted in an area under the receiver operating characteristic curve (AUC) of 0.95, 0.90, 0.92, and 0.81 for differentiating the SHR rats from the normotensive rats. This dysfunction in energy metabolism localizes to the medulla, the lower part of brain stem, and is, therefore, likely to contribute to the development, as well as to pathophysiological complications of hypertension

    Baseline data.

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    <p>The P values with “*”were obtained using Fisher’s Exact Test, whereas the rest were the result of independent samples t-test.</p

    Localization of the acupuncture specific effects by comparing TE5 verum acupuncture (Group A) vs. TE5 sham acupuncture (Group B) and TE5 verum acupuncture (Group A) vs. nonacupoint acupuncture (Group C).

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    <p>Abbreviation: BA, Brodmann area; Vox, voxel (represents the number of voxels); N/A, not available (means that the peak voxel was out of the BA zone). The BA area marked by “*”was corrected by a neurological physician.</p

    Differences of seed associated networks between ROIs from the left hemisphere and the right hemisphere.

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    <p>Full line represents stronger correlation under acupuncture compared with sham acupuncture, whereas the “dash–dot–dot” line represents weaker correlation. Dash line stands for weaker correlation compared with nonacupoint acupuncture (P<0.05, multiple comparison error corrected using Monte Carlo simulation). Regions of the left hemisphere and right hemisphere that had significant differences in correlation with seeds are placed on left side and right side, respectively, and ROIs in the same box are from the same hemisphere.</p

    The expression of protein spots in the medulla of four different groups of rats.

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    *<p><i>P</i><0.01, model group vs. Taichong point group or normal group;</p>#<p><i>P</i><0.01, Taichong group vs. non-acupoint group;</p>â–ľ<p><i>P</i><0.01, Taichong group vs. normal group;</p>â–´<p><i>P</i><0.01, non-acupoint group vs. model group; and <sup>â–Ş</sup><i>P</i><0.01, non-acupoint group vs. normal group.</p
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