29 research outputs found
Data_Sheet_1_Correlation between sarcopenia and cirrhosis: a meta-analysis.docx
BackgroundThe relationship between sarcopenia and cirrhosis is unclear. In this research, our aim is to evaluate the prevalence of sarcopenia among individuals with liver cirrhosis and its correlation with survival and mortality risks.MethodsWe conducted searches on PubMed, Web of Science, EMBASE, and Cochrane for English articles published up to July 10, 2023, and additionally manually searched the bibliography of relevant articles. We incorporated research on sarcopenia in patients with cirrhosis to examine the connection between sarcopenia and the likelihood of survival and mortality. Statistical analyses were carried out utilizing the Stata version 15.1 software. Depending on the heterogeneity of the results, we employed either fixed-effects models or random-effects models for data synthesis. To assess publication bias, we employed funnel plots and conducted Egger’s test.ResultsWe included 40 studies involving 8,945 patients with cirrhosis. The overall prevalence of cirrhosis was 41% (95% CI 34%–48%). Male patients and those with liver cirrhosis and hepatic encephalopathy had a higher prevalence of sarcopenia (44% for male patients and 48% for hepatic encephalopathy patients). Sarcopenia emerged as a risk factor for both survival (HR = 2.57, 95% CI 2.02–3.27, p ConclusionThis systematic review and meta-analysis reveal that patients with cirrhosis have a prevalence of sarcopenia of 41% and is associated with survival rate and mortality rate. Therefore, we should attach importance to the screening of sarcopenia in patients with cirrhosis, early detection of susceptible populations, and appropriate measures to reduce the occurrence and adverse outcomes.Systematic review registration:https://www.crd.york.ac.uk/PROSPERO/#recordDetails.</p
Citrate Synthase Expression Affects Tumor Phenotype and Drug Resistance in Human Ovarian Carcinoma
<div><p>Citrate synthase (CS), one of the key enzymes in the tricarboxylic acid (TCA) cycle, catalyzes the reaction between oxaloacetic acid and acetyl coenzyme A to generate citrate. Increased CS has been observed in pancreatic cancer. In this study, we found higher CS expression in malignant ovarian tumors and ovarian cancer cell lines compared to benign ovarian tumors and normal human ovarian surface epithelium, respectively. <i>CS</i> knockdown by RNAi could result in the reduction of cell proliferation, and inhibition of invasion and migration of ovarian cancer cells in vitro. The drug resistance was also inhibited possibly through an excision repair cross complementing 1 (ERCC1)-dependent mechanism. Finally, upon <i>CS</i> knockdown we observed significant increase expression of multiple genes, including <i>ISG15</i>, <i>IRF7</i>, <i>CASP7</i>, and <i>DDX58</i> in SKOV3 and A2780 cells by microarray analysis and real-time PCR. Taken together, these results suggested that CS might represent a potential therapeutic target for ovarian carcinoma.</p></div
Up regulated expression of citrate synthase (CS) in human ovarian tumors and human ovarian tumor cell lines.
<p>(<b>A</b>) <i>CS</i> mRNA and (<b>B</b>) protein expression was assessed in normal human ovarian surface epithelium (HOSE), ovarian cancer cell lines, benign (n = 11) and malignant ovarian tumors (n = 21) using real-time PCR and western blot, respectively (B =  ovarian benign tumor, M =  ovarian malignant tumor). Mean ± SEM. **<i>P</i><0.01 and ***<i>P</i><0.001.</p
<i>CS</i> silencing inhibits SKOV3 and A2780 cell invasion and migration in <i>vitro</i>.
<p>48 h after transfection, cell invasiveness was evaluated using the transwell assay and the Boyden Chamber test was used to measure the extent of cell migration. The number of invaded and migrated cells was counted using a bright-field microscope (200×). Representative images and the relative cell numbers were shown in (<b>A</b>) and (<b>B</b>). (<b>C</b>) p-FAK, MMP2 and Vimentin in <i>CS</i> knockdown cancer cells were analyzed using western blot. Mean ± SEM. *<i>P</i><0.05.</p
<i>CS</i> silencing affects AMPK/P38 MAPK pathway in ovarian cancer cell lines.
<p>(<b>A</b>) mRNA and <b>(B)</b> protein expression level of CS by real-time PCR and western blot in SKOV3 and A2780 cells after <i>CS</i> siRNA (100 nM) for 24 h and 48 h after transfection, respectively. (<b>C</b>) Decreased CS activity after 48 h transfection in SKOV3 and A2780 cells. (<b>D</b>) ATP level was examined 48 h after <i>CS</i> silencing. (<b>E, F</b>) p-AMPKα and p-p38 were analyzed in <i>CS</i>-silenced cancer cells by western blot. Mean ± SEM. *<i>P</i><0.05, **<i>P</i><0.01 and ***<i>P</i><0.001.</p
<i>CS</i> silencing results in proliferation reduction in SKOV3 and A2780 cells.
<p>(<b>A</b>) Cell proliferation was measured in <i>CS</i>-silenced cancer cells and control groups. (<b>B</b>) p-ERK and <b>(C)</b> Bcl-2 expression were measured after <i>CS</i> siRNA for 48 h in SKOV3 and A2780 cells by western blot. β-tubulin was used as a loading control. (<b>D</b>) 48 h after <i>CS</i> siRNA, cells were treated with indicated concentrations of DDP (15 µg/mL or 10 µg/mL) for 24 h. Cleaved caspase 3 was measured by western blot. Mean ± SEM. *<i>P</i><0.05 and **<i>P</i><0.01.</p
Gene expression profile related to drug resistance and apoptosis in SKOV3 and A2780 cells after <i>CS</i> silencing.
<p>(<b>A, B</b>) Ovarian cancer cells were treated with siRNA for 48 h, total RNA was extracted for gene expression analysis. Expression of <i>CASP7 (CASPASE7), IRF7 (</i>interferon regulatory factor 7), <i>DDX58 (</i>DEAD (Asp-Glu-Ala-Asp) box polypetide 58<i>)</i>, and <i>ISG15 (IFN</i>-stimulated gene 15<i>)</i> was increased significantly whereas <i>ATG12 (</i>autophagy related 12<i>)</i> expression was decreased in SKOV3 and A2780 cells after <i>CS</i> knockdown. <i>β-actin</i> was used as an internal control gene. Mean ± SEM. *<i>P</i><0.05, **<i>P</i><0.01 and ***<i>P</i><0.001. (<b>C</b>) The diagram was shown to identify potential signaling pathways modulated by CS.</p
<i>CS</i> silencing increases drug sensitivity in ovarian cancer cells.
<p>(<b>A</b>) Protein level of CS was examined in <i>CS</i>-silenced SKOV3 and A2780 cells. (<b>B</b>) SKOV3 and A2780 cells were treated with <i>CS</i> siRNA or negative siRNA for 48 h, then cells were treated with different concentrations of DDP for another 24 h. Cell viability was measured after incubation with CCK8 for 1.5 h. (<b>C</b>) 48 h after <i>CS</i> siRNA transfection, SKOV3 and A2780 cells were treated with 1 µg/ml DDP for 12 h and 1 h, respectively. Then cells were plated in 6-well plates, colonies were stained and counted after incubation for 8 days. Results shown were representative of three independent experiments. (<b>D</b>) SKOV3 and A2780 cells were treated with <i>CS</i> siRNA for 48 h, ERCC1 and γ-H2AX protein levels were compared between NC and siCS1078 group with β-tubulin used as a loading control. Mean ± SEM. *<i>P</i><0.05, **<i>P</i><0.01 and ***<i>P</i><0.001.</p
Table_2_Linguistic measures of personality in group discussions.DOCX
This investigation sought to find the relationships among multiple dimensions of personality and multiple features of language style. Unlike previous investigations, after controlling for such other moderators as culture and socio-demographics, the current investigation explored those dimensions of naturalistic spoken language that most closely align with communication. In groups of five to eight players, participants (N = 340) from eight international locales completed hour-long competitive games consisting of a series of ostensible missions. Composite measures of quantity, lexical diversity, sentiment, immediacy and negations were measured with an automated tool called SPLICE and with Linguistic Inquiry and Word Count. We also investigated style dynamics over the course of an interaction. We found predictors of extraversion, agreeableness, and neuroticism, but overall fewer significant associations than prior studies, suggesting greater heterogeneity in language style in contexts entailing interactivity, conversation rather than solitary message production, oral rather than written discourse, and groups rather than dyads. Extraverts were found to maintain greater linguistic style consistency over the course of an interaction. The discussion addresses the potential for Type I error when studying the relationship between language and personality.</p
Table_4_Linguistic measures of personality in group discussions.docx
This investigation sought to find the relationships among multiple dimensions of personality and multiple features of language style. Unlike previous investigations, after controlling for such other moderators as culture and socio-demographics, the current investigation explored those dimensions of naturalistic spoken language that most closely align with communication. In groups of five to eight players, participants (N = 340) from eight international locales completed hour-long competitive games consisting of a series of ostensible missions. Composite measures of quantity, lexical diversity, sentiment, immediacy and negations were measured with an automated tool called SPLICE and with Linguistic Inquiry and Word Count. We also investigated style dynamics over the course of an interaction. We found predictors of extraversion, agreeableness, and neuroticism, but overall fewer significant associations than prior studies, suggesting greater heterogeneity in language style in contexts entailing interactivity, conversation rather than solitary message production, oral rather than written discourse, and groups rather than dyads. Extraverts were found to maintain greater linguistic style consistency over the course of an interaction. The discussion addresses the potential for Type I error when studying the relationship between language and personality.</p