Additional file 1: of Comparison of endoscopic evacuation, stereotactic aspiration and craniotomy for the treatment of supratentorial hypertensive intracerebral haemorrhage: study protocol for a randomised controlled trial

SPIRIT Checklist. (PDF 83 kb

Association of VEGF Genetic Polymorphisms with Recurrent Spontaneous Abortion Risk: A Systematic Review and Meta-Analysis

<div><p>Background</p><p>Studies of the associations between the genetic polymorphisms of the vascular endothelial growth factor (VEGF) gene and recurrent spontaneous abortion (RSA) have revealed conflicting results. The present meta-analysis was performed to provide a more precise estimation of these relationships and to explore potential sources of heterogeneity that may have influenced the reported disparities.</p><p>Methods</p><p>An extensive literature search for relevant studies was conducted on PubMed, Embase, and The Cochrane Library through June 6, 2014. Crude odds ratio (OR) with 95% confidence intervals were calculated.</p><p>Results</p><p>10 case-control studies including 1,832 RSA patients and 2,271 healthy controls were identified. Meta-analysis indicated that rs1570360, rs3025039, rs2010963, and rs3025020 polymorphisms in the VEGF gene correlated with elevated RSA risk. The rs1570360 variant was statistically significantly relevant to RSA risk among non-Asian populations. Interestingly, the rs3025039 variant was statistically significantly relevant to RSA risk among Asian populations.</p><p>Conclusions</p><p>The current meta-analysis indicates that rs1570360, rs3025039, rs2010963, and rs3025020 polymorphisms increase RSA susceptibility. Moreover, rs1570360 and rs3025039 polymorphisms may play various roles in RSA susceptibility in various geographic groups.</p></div

Meta-analysis results for the five studied polymorphisms and RSA risk.

<p>^a REM: random-effect model. FEM: fixed-effect model.</p><p>P-value of overall effect.</p><p>Meta-analysis results for the five studied polymorphisms and RSA risk.</p

Synthesis of π-Extended Dithienobenzodithiophene-Containing Medium Bandgap Copolymers and Their Photovoltaic Application

<div><p>Two medium bandgap alternating conjugated copolymers, namely, poly{5,10-di(2-hexyldecyloxy)dithieno[2,3-<i>d</i>:2′,3′-<i>d</i>′]benzo[1,2-<i>b</i>:4,5-<i>b</i>′]dithiophene-2,7-diyl-<i>alt</i>-thiophene-2,5-di-yl} (<b>P1</b>) and poly{5,10-di(2-hexyldecyloxy)dithieno[2,3-<i>d</i>:2′,3′-<i>d</i>′]benzo[1,2-<i>b</i>:4,5-<i>b</i>′]dithiophene-2,7-di-yl-<i>alt</i>-thieno[3,2-<i>b</i>]thiophene-2,5-diyl} (<b>P2</b>), were prepared by the palladium-catalyzed Stille polycondensation and characterized by gel permeation chromatography (GPC), UV-Vis absorption and photoluminescence (PL) spectra, thermal gravimetric analysis (TGA), cyclic voltammetry (CV) <i>etc</i>. The resultant copolymers show moderate solubility in common organic solvents and enough stabilities for photovoltaic application. And both copolymers absorb the solar light from 300–600 nm, with the optical band gaps () calculated from the onset of absorption in the solid film of ca. 2.1 eV. The highest occupied molecular orbital (HOMO) levels of two copolymers determined by CV were at about −5.35 eV. Photovoltaic propertites of the polymers were investigated by using the polymers as donor and [6,6]-phenyl-C₇₁ butyric acid methyl ester (PC₇₁BM) as acceptor with a weight ratio of polymer:PC₇₁BM of 1:2. The power conversion efficiencies (PCEs) of polymer solar cells based on PDTBTT-TT reached 2.50%, with an open-circuit voltage (<i>V</i>_oc) of 0.70 V, a short-circuit current density (<i>J</i>_sc) of 6.89 mA cm⁻², and a fill factor (<i>FF</i>) of 52%, under the illumination of AM1.5, 100 mW cm⁻². These results indicate that dithieno[2,3-<i>d</i>:2′,3′-<i>d</i>′]benzo[1,2-<i>b</i>:4,5-<i>b</i>′]dithiophene (DTBDT) is a promising building block for the high-performance organic electronic materials.</p></div

Flow chart of literature search and study selection.

<p>Flow chart of literature search and study selection.</p

Main characteristics of the studies included in the meta-analysis.

<p>^aGenotype, for rs2010963, GG/GC/CC; for rs3025020, TT/CT/CC; for rs3025039, TT/CT/CC, for rs699947, AA/AC/CC, for rs833061, CC/CT/TT; and for rs1570360, AA/GA/GG.</p><p>^b HWE, Hardy-Weinberg equilibrium.</p><p>PCR, polymerase chain reaction; RFLP, restriction fragment length polymorphism.</p><p>Main characteristics of the studies included in the meta-analysis.</p

Forest plots for the associations between rs3025020 polymorphisms in the VEGF gene and RSA risk using.

<p>(A) codominant genetic models (C vs. T); (B) homozygous genetic models (CC vs. TT); (C) heterozygous genetic models (TT vs. CT); (D) dominant genetic models (TT+CT vs. CC); and (E) recessive genetic models (TT vs. CT+CC).</p

Forest plots for the associations between rs1570360 polymorphism in the VEGF gene and RSA risk using.

<p>(A) codominant genetic models (G vs. A); (B) (D) dominant genetic models (GG+GA vs. AA).</p

Sensitivity analysis for the associations between polymorphisms in the VEGF gene and RSA risk.

<p>(A) Sensitivity analysis for rs1570360 and RSA risk; (B) Sensitivity analysis for rs3025039 and RSA risk; (C) Sensitivity analysis for rs699947 and RSA risk; (D) Sensitivity analysis for rs2010963 and RSA risk; and (E) Sensitivity analysis for rs3025020 and RSA risk.</p

Types of oocytes from guinea pigs and their meiotic progression during IVM.

The arrow indicates the first polar body of the matured oocytes, indicating the oocytes have entered the MII phase. (A) Matured oocytes stained with Cell Truck Bule CMF2HC under the microscope after treatment with ROS for 12 hours. (B) Immature oocytes stained with Cell Truck Bule CMF2HC under the microscope after treatment with ROS for 12 hours. (C) After the removal of IMBX, the fluorescence intensity of matured oocytes treated with IBMX was significantly stronger than that treated with ROS. (D) The fluorescence intensity of GSH in MII oocytes cultured in the maturation medium was relatively weaker, and the first polar body was not obvious as well, compared with that cultured in the maturation medium with ROS. (E) The fluorescence intensity of GSH in MII oocytes cultured in MTA medium supplemented with L-cysteine and cystine was stronger than that of GSH in MII oocytes cultured in basic medium. (F) The fluorescence intensity of GSH cultured in the basic medium was much lower than other groups.</p