Additional file 4 of Pan-cancer analysis of the oncogenic role of discs large homolog associated protein 5 (DLGAP5) in human tumors

Additional file 4: Figure S4. Correlation between DLGAP5 gene expression and survival prognosis of cancers in TCGA. a The GEPIA2 tool to perform overall survival analyses showed LIHC, LUAD, LUSC, MESO, PAAD, and UVM in TCGA by DLGAP5 gene expression. b The GEPIA2 tool to perform disease-free survival analyses showed MESO, PAAD, PRAD, RARC, THCA, and UVM in TCGA by DLGAP5 gene expression

Additional file 6 of Pan-cancer analysis of the oncogenic role of discs large homolog associated protein 5 (DLGAP5) in human tumors

Additional file 6: Figure S6. Relationship between DLGAP5 gene and OS of cancers using the SangerBox tool. We used the SangerBox toll to perform OS analyses indifferent cancers of TCGA

Image_1_Androgen Receptors Act as a Tumor Suppressor Gene to Suppress Hepatocellular Carcinoma Cells Progression via miR-122-5p/RABL6 Signaling.tif

Hepatocellular carcinoma (HCC) is a malignant tumor with a high degree of malignancy and a poor prognosis. Androgen receptor (AR) has been reported to play important roles in the regulation of the progression of HCC, but the underlying mechanisms of how AR regulates HCC initiation, progression, metastasis, and chemotherapy resistance still need further study. Our study found that AR could act as a tumor suppression gene to suppress HCC cells invasion and migration capacities via miR-122-5p/RABL6 signaling, and the mechanism study further confirmed that miR-122-5p could suppress the expression of RABL6 to influence HCC cells progression by directly targeting the 3’UTR of the mRNA of RABL6. The preclinical study using an in vivo mouse model with orthotopic xenografts of HCC cells confirmed the in vitro data, and the clinical data gotten from online databases based on TCGA samples also confirmed the linkage of AR/miR-122-5p/RABL6 signaling to the HCC progression. Together, these findings suggest that AR could suppress HCC invasion and migration capacities via miR-122-5p/RABL6 signaling, and targeting this newly explored signaling may help us find new therapeutic targets for better treatment of HCC.</p

Additional file 9 of Pan-cancer analysis of the oncogenic role of discs large homolog associated protein 5 (DLGAP5) in human tumors

Additional file 9: Figure S9. DLGAP5 promoted HCC cells proliferation. a Real-time PCR identified the mRNA expression of DLGAP5 in PLC/PRF5-DLGAP5 cells, Hep3B-shDLGAP5-1,-2,-3 cells and their control cells. b western blot identified the protein expression of DLGAP5 in PLC/PRF5-DLGAP5 cells, Hep3B-shDLGAP5-1,-2,-3 cells and their control cells. c Proliferation of PLC/PRF5-DLGAP5, Hep3B-shDLGAP5-3 cells and control cells was examined by MTT

Additional file 10 of Pan-cancer analysis of the oncogenic role of discs large homolog associated protein 5 (DLGAP5) in human tumors

Additional file 10: Figure S10. DLGAP5 promoted HCC cells migration. Wound-healing assay were subjected to detect the migration capacity of DLGAP5-interfered cells

Additional file 1 of Pan-cancer analysis of the oncogenic role of discs large homolog associated protein 5 (DLGAP5) in human tumors

Additional file 1: Figure S1. Genomic location of human DLGAP5 and expression in different cancers and cells. a Genomic location of human DLGAP5; b The expression of DLGAP5 in diferent cancers; c DLGAP5 is high expression in different cancer cell lines

Additional file 11 of Pan-cancer analysis of the oncogenic role of discs large homolog associated protein 5 (DLGAP5) in human tumors

Additional file 11: Figure S11. Correlation analysis between DLGAP5 expression and immune infiltration of CD8+ T-cells. Different algorithms were used to explore the potential correlation between the expression level of DLGAP5 gene and the infiltration level of CD8+ T-cells across all types of cancer in TCGA

Additional file 13 of Pan-cancer analysis of the oncogenic role of discs large homolog associated protein 5 (DLGAP5) in human tumors

Additional file 13. Materials and methods

Additional file 2 of Pan-cancer analysis of the oncogenic role of discs large homolog associated protein 5 (DLGAP5) in human tumors

Additional file 2: Figure S2. Expression level of the DLGAP5 gene in different tumors and pathological stages. a and b The expression statuses of the DLGAP5 gene in SARC, CESC, PAAD, SKCM, TGCT, UCS ACC, LGG in TCGA project were compared with the corresponding normal tissues of the GTEx databases. c Expression levels of the DLGAP5 gene by different pathological stages of COAD, KICH, LUAD, LUSC; d BRCA, PAAD, SKCM, THCA; e BLCA, CESC, CHOL, DLBC; f ESCA, HNSC, OV, PEAD; and g STAD, TGCT, UCEC, UCS

Additional file 3 of Pan-cancer analysis of the oncogenic role of discs large homolog associated protein 5 (DLGAP5) in human tumors

Additional file 3: Figure S3. Pooled analysis on the DLGAP5 expression difference between normal and tumor tissues via the Oncomine database. a Colorectal cancer; b sarcoma cancer; c breast cancer; d lung cancer