107 research outputs found
Purification of manganese peroxidase from <i>Irpex lacteus</i> CD2.
<p>Purification of manganese peroxidase from <i>Irpex lacteus</i> CD2.</p
Decolorization of simulated textile wastewater (10%, 30%, 50%) by purified CD2-MnP for 72 h.
<p>RBV5R: Remazol Brilliant Violet 5R; DR5B: Direct Red 5B; RBBR: Remazol Brilliant Blue R; IC: Indigo Carmine.</p><p>Decolorization of simulated textile wastewater (10%, 30%, 50%) by purified CD2-MnP for 72 h.</p
Induction, Purification and Characterization of a Novel Manganese Peroxidase from <i>Irpex lacteus</i> CD2 and Its Application in the Decolorization of Different Types of Dye
<div><p>Manganese peroxidase (MnP) is the one of the important ligninolytic enzymes produced by lignin-degrading fungi which has the great application value in the field of environmental biotechnology. Searching for new MnP with stronger tolerance to metal ions and organic solvents is important for the maximization of potential of MnP in the biodegradation of recalcitrant xenobiotics. In this study, it was found that oxalic acid, veratryl alcohol and 2,6-Dimehoxyphenol could stimulate the synthesis of MnP in the white-rot fungus <i>Irpex lacteus</i> CD2. A novel manganese peroxidase named as CD2-MnP was purified and characterized from this fungus. CD2-MnP had a strong capability for tolerating different metal ions such as Ca<sup>2+</sup>, Cd<sup>2+</sup>, Co<sup>2+</sup>, Mg<sup>2+</sup>, Ni<sup>2+</sup> and Zn<sup>2+</sup> as well as organic solvents such as methanol, ethanol, DMSO, ethylene glycol, isopropyl alcohol, butanediol and glycerin. The different types of dyes including the azo dye (Remazol Brilliant Violet 5R, Direct Red 5B), anthraquinone dye (Remazol Brilliant Blue R), indigo dye (Indigo Carmine) and triphenylmethane dye (Methyl Green) as well as simulated textile wastewater could be efficiently decolorized by CD2-MnP. CD2-MnP also had a strong ability of decolorizing different dyes with the coexistence of metal ions and organic solvents. In summary, CD2-MnP from <i>Irpex lacteus</i> CD2 could effectively degrade a broad range of synthetic dyes and exhibit a great potential for environmental biotechnology.</p></div
Decolorization of different types of dyes by the purified CD2-MnP with the coexistence of organic solvents.
<p>The reaction mixture in a total volume 1 ml contained (final concentration): malonate buffer (20 mM, pH 4.5), Mn<sup>2+</sup> (1.6 mM), H<sub>2</sub>O<sub>2</sub> (0.08 mM), purified CD2-MnP (0.25 U/ml), dye (50 mg/L) and methanol, DMSO, ethylene glycol, glycerin (20%). <b>CK (MnP+H<sub>2</sub>O<sub>2</sub>)</b> was the control without addition of any organic solvent. (<b>A</b>): Decolorization of RBV5R; (<b>B</b>): Decolorization of DR5B; (<b>C</b>): Decolorization of RBBR; (<b>D</b>): Decolorization of IC. (<b>E</b>): Decolorization of MG. <b>RBV5R</b>: Remazol Brilliant Violet 5R, <b>DR5B</b>: Direct Red 5B, <b>RBBR</b>: Remazol Brilliant Blue R, <b>IC</b>: Indigo Carmine, <b>MG</b>: Methyl Green.</p
Effect of pH and temperature on the activity and stability of purified CD2-MnP from <i>Irpex lacteus</i> CD2.
<p><b>A</b>: Effect of pH on MnP activity. The activity of 100% was that which was measured at the optimal pH. <b>B</b>: Effect of pH on the stability of CD2-MnP. The initial MnP activity before incubation was set as 100%. <b>C</b>: Effect of temperature on MnP activity. The activity of 100% was that which was measured at the optimal temperature. <b>D</b>: Effect of temperature on the stability of CD2-MnP. The initial MnP activity before incubation was set as 100%.</p
SDS-PAGE analysis of the purified MnP from <i>Irpex lacteus</i> CD2.
<p>lane M: molecular mass marker; lane 1 and lane 2: purified MnP.</p
Decolorization of different types of dyes by the purified CD2-MnP with the coexistence of metal ions.
<p>The reaction mixture in a total volume 1 ml contained (final concentration): malonate buffer (20 mM, pH 4.5), Mn<sup>2+</sup> (1.6 mM), H<sub>2</sub>O<sub>2</sub> (0.08 mM), purified CD2-MnP (0.25 U/ml), dye (50 mg/L) and Ca<sup>2+</sup>, Co<sup>2+</sup>, Mg<sup>2+</sup>, Zn<sup>2+</sup> (4 mM). <b>CK (MnP+H<sub>2</sub>O<sub>2</sub>)</b> was the control without addition of any metal compound except Mn<sup>2+</sup>. <b>H<sub>2</sub>O<sub>2</sub> (no MnP)</b> was the negative control without addition of purified CD2-MnP. (<b>A</b>): Decolorization of RBV5R; (<b>B</b>): Decolorization of DR5B; (<b>C</b>): Decolorization of RBBR; (<b>D</b>): Decolorization of IC; (<b>E</b>): Decolorization of MG. <b>RBV5R</b>: Remazol Brilliant Violet 5R, <b>DR5B</b>: Direct Red 5B, <b>RBBR</b>: Remazol Brilliant Blue R, <b>IC</b>: Indigo Carmine, <b>MG</b>: Methyl Green. The negative control (H<sub>2</sub>O<sub>2</sub> was added into the decolorization mixture in the absence of purified CD2-MnP) showed no significant decolorization of different dyes.</p
Effect of metal ions and organic solvents on the activity of purified CD2-MnP.
<p><b>A</b>: The effect of different metal ions on MnP activity. The MnP activity of the control without adding any metal compound was set as 100%. <b>B</b>: The effect of different organic solvents on MnP activity. The MnP activity of the control without adding any organic solvent was set as 100%.</p
Effect of metal ions on the stability of purified CD2-MnP from <i>Irpex lacteus</i> CD2.
<p>The MnP activity of control without adding any metal compound was set as 100%.</p><p>Effect of metal ions on the stability of purified CD2-MnP from <i>Irpex lacteus</i> CD2.</p
UV-visible spectrum of the purified CD2-MnP.
<p>UV-visible spectrum of the purified CD2-MnP.</p
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