Tabular expression-based testing strategies: a comparison

Tabular expressions were proposed as a documentation tool that can be used to document software precisely and unambiguously. This paper explores the applications of four testing strategies in tabular expression-based specifications and further compares the strategies on a mathematical basis

LncRNA DGCR5/miR-204-5p/SRSF7 axis regulates PDGF-BB-induced proliferation and migration of airway smooth muscle cells with potential role in asthma

Increased proliferation and migration of abnormal airway smooth muscle cells (ASMCs) are significantly associated with asthma. Recently, the function of long non-coding RNAs (lncRNAs) in ASMCs has been identified. Our research attempted to resolve the function of the lncRNA DGCR5 in ASMCs and focus on its in-depth mechanisms of proliferation and migration. Quantitative real-time PCR and western blotting were used to evaluate the expressions of RNA and proteins, respectively. The CCK-8 assay was used to detect cell proliferation. Transwell assays were used to assess migration. Luciferase and RNA pull-down assays were used to verify the targeting between miR-204-5p and lncRNA DGCR5 or SRSF7. LncRNA DGCR5 was upregulated in ASMCs stimulated with PDGF-BB. Knockdown of DGCR5 reversed the effect of platelet-derived growth factor BB (PDGF-BB) on the proliferation and migration of ASMCs. The results of this study showed that lncRNA DGCR5 binds to miR-204-5p, and SRSF7 is the target of miR-204-5p. Rescue experiments verified the interaction between miR-204-5p and DGCR5, as well as that between miR-204-5p and SRSF7. Overall, this study revealed that the lncRNA DGCR5/miR-204-5p/SRSF7 signalling axis has key regulatory effects on the proliferation and migration of ASMCs.</p

Incorporating of Oxidized Cellulose Nanofibers@d‑Limonene Pickering Emulsion into Chitosan for Fully Biobased Coatings toward Fruits Protection

Due to the perishable nature and limited shelf life, it is crucial to develop highly efficient techniques for fruit preservation while avoiding the use of environmentally hazardous materials. Herein, the ecofriendly oxidized cellulose nanofibers (OCNF) with an optimized solid content (1.0 wt %) was utilized to stabilize d-Limonene to form an oil-in-water Pickering emulsion. By incorporating the Pickering emulsion into chitosan (Cs) solution, fully biobased Cs/OCNF@d-Limonene coatings were successfully obtained via a simple solvent casting method. Thanks to the physical cross-linking and hydrogen bond reaction between OCNF and Cs matrix, the films exhibited high transmittance, high haze, exceptional mechanical properties, and the ability to effectively shield 96.5% UVB and 69.3% UVA radiation. Most importantly, the inclusion of d-Limonene exhibited a remarkable enhancement in the inhibition zones against E. coli and S. aureus, and an outstanding 72.5% improvement in antioxidation properties. These outstanding film properties make the Cs/OCNF@d-Limonene coatings ideal as conformal films for fruit protection, which can effectively reduce the fruit water loss rate, delay ripening, prevent oxidation and microbial invasion, and significantly extend the shelf life of fruits. The well-designed coatings present a promising, cost-effective, and sustainable alternative to conventional plastic packaging materials for fruit protection

Additional file 1 of Association between central venous pressure measurement and outcomes in critically ill patients with severe coma

Additional file 1: Table S1. Missing value for included variables in this study. Table S2. Full multivariate model assessing the impact of central venous pressure on in hospital mortality in the original cohort. Table S3. Full multivariate model assessing the impact of central venous pressure on in hospital mortality in the PSM cohort. Table S4. Full multivariate model assessing the impact of central venous pressure on in hospital mortality in the IPTW cohort. Table S5. Full multivariate model assessing the impact of central venous pressure on in hospital mortality in the sIPTW cohort

MRNDR: Multihead Attention-Based Recommendation Network for Drug Repurposing

As is well-known, the process of developing new drugs is extremely expensive, whereas drug repurposing represents a promising approach to augment the efficiency of new drug development. While this method can indeed spare us from expensive drug toxicity and safety experiments, it still demands a substantial amount of time to carry out precise efficacy experiments for specific diseases, thereby consuming a significant quantity of resources. Therefore, if we can prescreen potential other indications for selected drugs, it could result in substantial cost savings. In light of this, this paper introduces a drug repurposing recommendation model called MRNDR, which stands for Multi-head attention-based Recommendation Network for Drug Repurposing. This model serves as a prediction tool for drug-disease relationships, leveraging the multihead self-attention mechanism that demonstrates robust generalization capabilities. These capabilities stem not only from our extensive million-level training data set, BioRE (Biology Recommended Entity data), but also from the utilization of the WRDS (Weighted Representation Distance Score) algorithm proposed by us. The MRNDR model has achieved new state-of-the-art results on the GP-KG public data set, with an MRR (Mean Reciprocal Rank) score of 0.308 and a Hits@10 score of 0.628. This represents significant improvements of 4.7% (MRR) and 18.1% (Hits@10) over the current best-performing models. Additionally, to further validate the practical utility of the model, we examined results recommended by MRNDR that were not present in the training data set. Some of these recommendations have undergone clinical trials, as evidenced by their presence on ClinicalTrials.gov and the China Clinical Trials Center, indirectly confirming the applicability of MRNDR. The MRNDR model can predict the reusability of candidate drugs, reducing the need for manual expert assessments and enabling efficient drug repurposing

One-Pot Synthesis of Highly Substituted 4‑Acetonylindoles via Sequential Dearomatization and Silver-Catalyzed Domino Reaction

Synthetically useful 4-acetonylindoles have been conveniently prepared from 2-alkynylanilines and silyl enol ethers using a dearomatization strategy. The two-step/one-pot protocol involves an iodosylbenzene-mediated oxidative dearomatization and a silver-catalyzed domino reaction

Additional file 1 of Opioid exposure during pregnancy and the risk of congenital malformation: a meta-analysis of cohort studies

Additional file 1 Table S1. Search terms. Table S2. Summary of risk of bias assessment using ROBINS-I tool. Fig. S1. Eggers’s test of studies examining the association between opioids exposure and the risk of congenital malformations. Fig. S2. Forest plot of association between opioid exposure and major congenital malformation. Fig. S3. Forest plot of association between opioid exposure and central nervous system malformation. Fig. S4. Forest plot of association between opioid exposure and limb malformation. Fig. S5. Forest plot of assoxiation between opioid exposure and cardiovascular malformation. Fig. S6. Forest plot of association between opioid exposure and gastrointestinal malformation. Fig. S7. Forest plot of association between opioid exposure and ear, face, and neck malformation. Fig. S8. Forest plot of association between opioid exposure and respiratory malformation. Fig. S9. Forest plot of association between opioid exposure and musculoskeletal malformation. Fig. S10. Forest plot of association between opioid exposure and urogenital malformation. Fig. S11. Forest plot of association between opioid exposure and orofacial malformation. Fig. S12. Forest plot of association between opioid exposure and neural tube defects. Fig. S13. Forest plot of association between opioid exposure and gastroschisis. Fig. S14. Forest plot of association between opioid exposure and clubfoot. Fig. S15. Meta-regression according to the year of publication. Fig. S16. Forest plot of subgroup analysis of exposed period. Fig. S17. Forest plot of subgroup analysis of indication. Fig. S18: Forest plot of subgroup analysis of adjusted for confounders. Fig. S19. Forest plot of subgroup analysis of risk of bias assessment

One-Pot Synthesis of Highly Substituted 4‑Acetonylindoles via Sequential Dearomatization and Silver-Catalyzed Domino Reaction

Synthetically useful 4-acetonylindoles have been conveniently prepared from 2-alkynylanilines and silyl enol ethers using a dearomatization strategy. The two-step/one-pot protocol involves an iodosylbenzene-mediated oxidative dearomatization and a silver-catalyzed domino reaction

DataSheet1_Evolution and Trend Analysis of Research Hotspots in the Field of Pollution-Intensive Industry Transfer—Based on Literature Quantitative Empirical Study of China as World Factory.ZIP

Background: The transfer of pollution-intensive industries in China accounts for an increasing proportion of industrial transfer, and related studies emerge endlessly. Fully exploring its research and development breadth and depth will help clarify the development trend in this field and point out the direction for future research.Method/Process: From the perspective of bibliometric analysis, with keywords as the core and cluster analysis of research hotspots as the basis, the keywords of pollution-intensive industry transfer in CNKI database are analyzed by CiteSpace software and divided into five categories. Established the text corpus model, and the network analysis transformed into a visual form. Due to the diverse research hotspots in pollution-intensive industry transfer, this article analyzes the evolution of research hotspots in this field to predict its future development trend.Conclusion/Significance: China, as the world’s factory, is affected by relevant policies, and industrial transfers have generally occurred. Industrial transfer, especially the transfer of pollution-intensive industries, has gradually attracted the attention of academic circles and has become a hot topic. When dealing with the transfer of pollution-intensive industries, industrial transfer only transfers pollution across regions. If we want to reduce pollution from the origin, innovation is an essential means. In retrospect, there were rare articles concerning the emerging polluting industries, however, recently since the emerging polluting industries have already constituted as the main source of pollution, more academic attentions are definitely needed. Although the mainstream measurement methods, the related share index method, and the input-output table have their weaknesses, respectively, the deviation share method can overcome the shortcomings of both. Therefore, it can be used as a reference for scholars to measure the transfer of pollution-intensive industries in the future.</p

Direct Remote Asymmetric Bisvinylogous 1,4-Additions of Cyclic 2,5‑Dienones to Nitroalkenes

Here we report that cyclic 2,5-dienones can act as bisvinylogous precursors through in situ generation of linear trienamine species with a cinchona-derived primary amine, and exclusively remote ε-regioselective 1,4-additions to nitroalkenes were accomplished in moderate to high enantioselectivity. Moreover, a diversity of complex spirocyclic frameworks could be efficiently constructed in an enantioenriched manner from these multifunctional 1,4-adducts via subsequent vinylogous iminium and even cascade iminium catalysis of the same amine