Additional file 1 of Role of arachidonic acid metabolism in intervertebral disc degeneration: identification of potential biomarkers and therapeutic targets via multi-omics analysis and artificial intelligence strategies

Additional file 1

“Dandelion” Inspired Dual-Layered Nanoarrays with Two Model Releasing Features for the Surface Modification of 3D Printing Implants

Inspired from dandelion, dual-layered ZnO nanoarrays were modified on the surface of 3D printing implants to reduce the postoperation infective rate. According to both <i>in vitro</i> and <i>in vivo</i> tests, it exhibited unique two model-releasing features which could be applied to 3D printing implants as a healthier alternative to antibiotics

Additional file 3 of Long intergenic non-coding RNA DIO3OS promotes osteosarcoma metastasis via activation of the TGF-β signaling pathway: a potential diagnostic and immunotherapeutic target for osteosarcoma

Additional file 3: Table S1. Sequences of Ribo TM h-DIO3OS Smart Silencer. Table S2. Primers and sequences used in this study. Table S3. Baseline clinical characteristics of patients with osteosarcoma in validation cohort

Additional file 2 of Long intergenic non-coding RNA DIO3OS promotes osteosarcoma metastasis via activation of the TGF-β signaling pathway: a potential diagnostic and immunotherapeutic target for osteosarcoma

Additional file 2: Figure S1. Differential expression of DIO3OS in pan-cancer

Additional file 1 of Long intergenic non-coding RNA DIO3OS promotes osteosarcoma metastasis via activation of the TGF-β signaling pathway: a potential diagnostic and immunotherapeutic target for osteosarcoma

Additional file 1. Raw data

The “Pure Marriage” between 3D Printing and Well-Ordered Nanoarrays by Using PEALD Assisted Hydrothermal Surface Engineering

For the first time, homogeneous and well-ordered functional nanoarrays were grown densely on the complex structured three-dimensional (3D) printing frameworks through a general plasma enhanced atomic layer deposition (PEALD) assisted hydrothermal surface engineering process. The entire process was free from toxic additives or harmful residues and, therefore, can meet the critical requirements of high-purity products. As a practical example, 3D customized earplugs were precisely manufactured according to the model of ear canals at the 0.1 mm level. Meanwhile, well-ordered ZnO nanoarrays, formed on the surfaces of these 3D printed earplugs, could effectively prevent the growth of five main pathogens derived from the patients with otitis media and exhibited excellent wear resistance as well. On the basis of both animal experiments and volunteers’ investigations, the 3D customized earplugs showed sound insulation capabilities superior to those of traditional earplugs. Further animal experiments demonstrated the potential of as-modified implant scaffolds in practical clinical applications. This work, exemplified with earplugs and implant scaffolds, oriented the development direction of 3D printing in biomedical devices, which precisely integrated customized architecture and tailored surface performance

The “Pure Marriage” between 3D Printing and Well-Ordered Nanoarrays by Using PEALD Assisted Hydrothermal Surface Engineering

For the first time, homogeneous and well-ordered functional nanoarrays were grown densely on the complex structured three-dimensional (3D) printing frameworks through a general plasma enhanced atomic layer deposition (PEALD) assisted hydrothermal surface engineering process. The entire process was free from toxic additives or harmful residues and, therefore, can meet the critical requirements of high-purity products. As a practical example, 3D customized earplugs were precisely manufactured according to the model of ear canals at the 0.1 mm level. Meanwhile, well-ordered ZnO nanoarrays, formed on the surfaces of these 3D printed earplugs, could effectively prevent the growth of five main pathogens derived from the patients with otitis media and exhibited excellent wear resistance as well. On the basis of both animal experiments and volunteers’ investigations, the 3D customized earplugs showed sound insulation capabilities superior to those of traditional earplugs. Further animal experiments demonstrated the potential of as-modified implant scaffolds in practical clinical applications. This work, exemplified with earplugs and implant scaffolds, oriented the development direction of 3D printing in biomedical devices, which precisely integrated customized architecture and tailored surface performance