Data_Sheet_1_Association between the weight-adjusted-waist index and abdominal aortic calcification in United States adults: Results from the national health and nutrition examination survey 2013–2014.doc

BackgroundAbdominal aortic calcification (AAC) is recognized as a strong predictor of cardiovascular disease (CVD) events. This study aimed to evaluate the association between weight-adjusted-waist index (WWI) and AAC in United States adults aged ≥ 40 years.Materials and methodsData were derived from the 2013–2014 National Health and Nutrition Examination Survey (NHANES). WWI was calculated as waist circumference divided by the square root of weight. AAC scores were quantified by the Kauppila scoring system, and severe AAC was defined as an AAC score ≥ 6. Weighted multivariable regression analysis and subgroup analysis were performed to evaluate the relationship between WWI with AAC scores and severe AAC. The restricted cubic spline model was used for the dose-response analysis.ResultsA total of 2,772 participants were included with the mean WWI of 11.17 ± 0.73 cm/√kg and mean AAC score of 1.48 ± 3.27. The prevalence of severe AAC was 9.64%. WWI was positively associated with higher AAC scores [β = 0.95, 95% confidence interval (CI): 0.65–1.25, P ConclusionHigher WWI was associated with higher AAC score and increased risk of severe AAC in United States adults. Further studies are needed to confirm this relationship.</p

Coordination polymers constructed from 5-nitro-1,2,3-benzenetricarboxylic acid: crystal structures and treatment effect on nephrotic syndrome by regulating intestinal flora and recovering Th17/Treg balances

Two new transition metal-based coordination polymers [Zn3(bimb)2(nbta)2]n (1, bimb = 1,4-bis(imidazol-1-yl)-2-butene, H3nbta = 5-nitro-1,2,3-benzenetricarboxylic acid) and {[Cu3(nbta)2(bipp)2(H2O)2](H2O)2}n (2, bipp = 1,4-bis(pyridin-4-ylmethyl)piperazine) have been successfully prepared by reaction of 5-nitro-1,2,3-benzenetricarboxylic acid with the corresponding metal salts and the different N-donor ligands. Furthermore, the treatment effect of compounds 1 and 2 on nephrotic syndrome (NS) was evaluated and the specific mechanism was explored in this research. Firstly, to investigate the level of the Th17 cells and CD4 + CD25 + Foxp3+ regulate cells (Treg) in PBMC was detected with flow cytometry. The RT-PCR was performed to analyze the mRNA expression level of 16sRNA to determine the intestinal flora abundance.</p

Cation-π-Induced Exfoliation of Graphite by a Zwitterionic Polymeric Dispersant for Congo Red Adsorption

Water dispersion remains a technical challenge for effective applications of graphene. In this paper, graphene nanosheets were successfully produced by directly exfoliating expanded graphite in water in the presence of zwitterionic polymeric dispersant, succinic anhydride-functionalized polyethylenimine (PEI-SA). The effects of various parameters, including SA percentage in PEI-SA, dispersant concentration, pH, centrifugation speed, and initial graphite concentration on the exfoliation and stabilization of graphene were studied. Cation-π interaction between the protonated amines in PEI-SA and polyaromatic structure in graphite, together with the zwitterionic property and the steric hindrance of PEI-SA, facilitated the exfoliation and stabilization of graphene. Graphene nanosheets with negligible defects were obtained by dispersant-assisted liquid-phase exfoliation. The concentration of exfoliated graphene suspension under the optimal condition was 0.56 mg/mL with the yield as high as 11.2% and the suspension remained stable for more than 6 months. The stability of exfoliated graphene suspension was a function of pH as determined by UV–vis absorbance, hydrodynamic size, and zeta potential. The exfoliated graphene showed greater potential as an adsorbent for Congo red (CR) removal from water compared to that of pristine graphene

Hierarchical clustering of lncRNAs differentially expressed in kidney between low birth weight and control rates.

<p>A hierarchical clustered heat map showing the log₂ transformed expression values for differentially expressed lncRNAs (absolute fold-change ≥2; <i>p</i> ≤0.05) between low birth weight rats (L) and normal controls (C). Three rats were analyzed for each group. The intensity of the color scheme is calibrated to the log₂ expression values, where red refers to high relative expression and green refers to low relative expression. The bar code represents the color scale of the log₂ values.</p

Additional file 2 of Derivation and validation of urinary TIMP-1 for the prediction of acute kidney injury and mortality in critically ill children

Additional file 2: Fig. S1. Comparison of the initial (a) and peak (b) urinary biomarkers among patients with AKI status and/or death in the discovery cohort. Each dot represents an individual patient; the horizontal lines indicate medium with interquartile range. *P<0.05 vs. survivors with non-AKI, #P<0.05 vs. survivors with AKI stage 1 or 2. AKI acute kidney injury, FABP-1 fatty acid binding protein 1, IGFBP7 insulin-like growth factor-binding protein 7, IP-10 interferon inducible protein-10, KIM-1 kidney injury molecule-1, NGAL neutrophil gelatinase-associated lipocalin, TFF-3 trefoil factor-3, TIMP-1 tissue inhibitor of metalloproteinases-1, TIMP-2 tissue inhibitor of metalloproteinases-2. Each dot represents an individual patient; the horizontal lines indicate the median with interquartile range. *P<0.05 vs. survivors with non-AKI, #P<0.05 vs. survivors with AKI Stage ½

Body weight and glomerular number in low birth weight rats compared with controls.

<p><b>A:</b> Body weight in low birth weight (LBW) rats compared with normal controls at postnatal day 1 (p1) and 10 (p10). Values are means with SD. <b>B:</b> Comparison of glomerular number between LBW and control rats at p10. Each circle represents an individual rat and the horizontal and vertical lines indicate the means and SD. <i>p</i> Value between LBW and control rats was calculated by the Mann-Whitney U-test, n <i>=</i> 7 for each group. <b>C:</b> Scatter plot showing the distribution of glomerular number based on birth weight. The number of glomeruli was positively correlated with body weight at birth. r = Spearman’s correlation coefficient. The open circles represent the three rats randomly selected for microarray analysis from each group.</p

Comparison of lncRNA expression in different tissues.

<p>Comparison of the expression of lncRNA TCONS_00017119 in different tissues from low birth weight and control rats at postnatal day 10 by quantitative real-time PCR. The level of lncRNA was calculated relative to GAPDH. The data are expressed as relative to lncRNA level in the brain of normal control rats. Values are means with SEM. <i>p</i> Value was calculated by the Student’s t-test. Black bars, control rats (n = 5); open bars, low birth weight rats (n = 5). *p <0.05 vs. low birth weight rats, ^#p <0.05 vs. normal kidney.</p

Correlation analysis of lncRNAs expression with glomerular number.

<p>The number of glomeruli was positively correlated with the relative expression of lncRNAs TCONS_0014139 (A: r = 0.88; <i>p</i> <0.001; n = 14) and TCONS_00014138 (B: r = 0.77; <i>p</i> = 0.001; n = 14), but negatively with lncRNA TCONS_00017119 (C: r = -0.80; <i>p</i> = 0.001; n = 14) in the kidneys at postnatal day 10 (p10). Black circles, control rats (n = 7); open circles, low birth weight rats (n = 7). The level of lncRNA was calculated relative to GAPDH. The data are expressed as relative to the controls at p10. r = Spearman’s correlation coefficient.</p

Relative differential lncRNA and mRNA expression in the kidneys between low birth weight and control rats at postnatal day 10 of life, detected using microarray.

<p>Relative differential lncRNA and mRNA expression in the kidneys between low birth weight and control rats at postnatal day 10 of life, detected using microarray.</p

Additional file 1 of Increased circulating innate lymphoid cell (ILC)1 and decreased circulating ILC3 are involved in the pathogenesis of Henoch-Schonlein purpura

Additional file 1