42 research outputs found

    Exogenous miR-129-5p inhibited cell proliferation and promoted apoptosis of Hela cells.

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    <p>Cell growth was measured by an MTT assay. The experiment was repeated at least twice, each with three replicates, and the data are expressed as mean absorbances with SD. *<i>P</i><0.001; #<i>P</i><0.05.</p

    MiR-129-5p can be induced by IFN-β in Hela cells.

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    <p>(A) Validation of the microarray data by qPCR. Triplicate assays were performed for each sample. Inductions of known IFN-regulated genes <i>ISG54</i> and <i>ISG56</i> are shown for comparsion. (B) Time course of miRNA induction by IFN-β. Hela cells were stimulated with 300 IU ml<sup>−1</sup> IFN-β for the indicated times, and <i>miR-129-5p</i> and <i>ISG54</i>/<i>ISG56</i> expressions were quantified by qPCR. (C) Dose-response analysis of miRNA induction by IFN-β. Hela cells were stimulated with the indicated doses of IFN-β for 2 h, and <i>miR-129-5p</i> and <i>ISG54</i>/<i>ISG56</i> expressions were quantified by qPCR.</p

    Increase of <i>miR-129-5p</i> expression in Hela cells decreased HPV-18 <i>E6</i> and <i>E7</i> viral gene expressions.

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    <p>Fig.(A) showed the efficiency of miR-129 transfection. Fold changes of the expressions of (B) HPV-18 <i>E6</i> and (C) HPV-18 <i>E7</i> in Hela cells determined by qPCR. The experiment was repeated twice, each with three replicates. Means (bars) and SDc (error bars) are shown. *<i>P</i><0.001.</p

    The expression of miRNA-129-5p and HPV-18 E6/E7 in human cervical intraepithelial lesion samples and normal cervical tissues.

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    <p>(A) The expression of miRNA-129-5p in human cervical intraepithelial lesion samples and normal cervical tissues. (B) The expression of HPV-18 E6 in the same samples. (C) The expression of HPV-18 E7 in these samples. (D, E) Correlation between the expressions of HPV-18 E6/E7 and miR-129 in these samples.</p

    Negative regulation of SP1 by miR-129-5p in Hela cells.

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    <p>(A) Vector restriction map. (B) SP1 was targeted by miR-129-5p via its 3′UTR in Hela cells as assessed using a luciferase assay. (C) Western blot analyses of p21, SP1 and GAPDH in Hela cells after miR-129-5p over-expression. Means (bars) and SD (error bars) are shown.*P<0.05.</p
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