Additional file 4 of Genome-wide analysis of long non-coding RNAs (lncRNAs) in two contrasting rapeseed (Brassica napus L.) genotypes subjected to drought stress and re-watering

Additional file 4. LncRNA and their putative target mRNA based on the co-expression results

Additional file 1 of Genome-wide analysis of long non-coding RNAs (lncRNAs) in two contrasting rapeseed (Brassica napus L.) genotypes subjected to drought stress and re-watering

Additional file 1. RNA-seq data for 12 samples

Additional file 9 of Genome-wide analysis of long non-coding RNAs (lncRNAs) in two contrasting rapeseed (Brassica napus L.) genotypes subjected to drought stress and re-watering

Additional file 9. List of primers for quantitative real-time PCR

Additional file 3 of Genome-wide analysis of long non-coding RNAs (lncRNAs) in two contrasting rapeseed (Brassica napus L.) genotypes subjected to drought stress and re-watering

Additional file 3. Relative expression data measured by qRT-PCRs and RNA-seq

The role of maintenance therapy in the treatment of advanced urothelial cancer: a comprehensive systematic review and network meta-analysis

Concerns increase with maintenance therapy for advanced urothelial cancer (aUC). We perform a comprehensive network meta-analysis (NMA) to investigate the efficacy and toxicities of maintenance therapy in aUC patients. Trials assessing maintenance treatment with either a continuous or a switch strategy for aUC were identified. The primary outcome was overall survival (OS), and secondary outcome was progression-free survival (PFS) and toxicities. Nine articles reporting eight trials were included. The pooled hazard ratio demonstrated that maintenance therapy significantly improved OS giving HR 0.83 (95%CI: 0.74–0.93, P = 0.0013) and PFS with HR of 0.78 (95%CI: 0.62–0.99, P = 0.05), but increased the risk of developing severe adverse events and treated-related discontinues (P < 0.05). Sub-group analysis indicated that ‘switch’ ICI (immune checkpoint inhibitor) maintenance therapy significantly improved OS and PFS when compared to best support care (BSC) (P < 0.05). NMA showed that chemotherapy followed by ‘switch’ maintenance with ICI significantly improved OS (HR 0.70, 95%CI: 0.57–0.87) when compared to BSC. ‘Continuous’ maintenance with ICI alone had a tendency to improve OS (HR 0.85, 95%CI: 0.71–1.01), and TA (HR0.93, 95%CI: 0.58–1.50) and vinflunine (HR 0.74, 95%CI: 0.44–1.24) was no significantly associated with a lower likelihood of disease death. Based on the analysis of the treatment ranking, ‘switch’ maintenance with ICI appeared as the best treatment approach. Our pooled results confirm that maintenance therapy yields a significant survival advantage for aUC patients. NMA indicates that switch maintenance with ICI is the optimum maintenance treatment for aUC and reduces mortality by about a third.</p

Additional file 5 of Genome-wide analysis of long non-coding RNAs (lncRNAs) in two contrasting rapeseed (Brassica napus L.) genotypes subjected to drought stress and re-watering

Additional file 5. The lncRNA-mRNA pairs reflected the opposite expression trend in two genotypes

Additional file 7 of Genome-wide analysis of long non-coding RNAs (lncRNAs) in two contrasting rapeseed (Brassica napus L.) genotypes subjected to drought stress and re-watering

Additional file 7. Differentially expressed lncRNAs in response to drought stress and re-watering

Additional file 2 of Genome-wide analysis of long non-coding RNAs (lncRNAs) in two contrasting rapeseed (Brassica napus L.) genotypes subjected to drought stress and re-watering

Additional file 2. RNA amount obtained from each treatment for RNA-seq analysis

DataSheet1_A novel near-infrared fluorescent probe for rapid sensing of HClO in living cells and zebrafish.docx

Reactive oxygen species (ROS) are significant active species in living organisms, and their coordination maintains the function of organelles to resist the invasion of foreign substances. Hypochlorous acid (HClO) is not only an eventful signaling species but also a kind of ROS, which plays an irreplaceable role in the immune system. However, its abnormal levels can cause cell damage or even apoptosis, which in turn leads to the onset of a series of diseases such as inflammation, neurological diseases, and even cancer. Based on this, we designed a near-infrared fluorescent probe with a large Stokes shift for ultrafast response to HClO. Furthermore, the probe exhibits excellent sensitivity and selectivity toward HClO over other species. The probe was successfully applied to visualize endogenous and exogenous HClO in living cells and in zebrafish. This unique study is the key to providing a trustworthy tool for imaging based on the in vitro and in vivo imaging of endogenous HClO, which possesses great potential for the use in future studies of HClO-related biology and pathology.</p

Additional file 8 of Genome-wide analysis of long non-coding RNAs (lncRNAs) in two contrasting rapeseed (Brassica napus L.) genotypes subjected to drought stress and re-watering

Additional file 8. The lncRNA-mRNA pairs in the lncRNA-mRNA co-expression network of plant hormone signal transduction