Dynamics of Oxygen-Independent Photocleavage of Blebbistatin as a One-Photon Blue or Two-Photon Near-Infrared Light-Gated Hydroxyl Radical Photocage

Development of versatile, chemically tunable photocages for photoactivated chemotherapy (PACT) represents an excellent opportunity to address the technical drawbacks of conventional photodynamic therapy (PDT) whose oxygen-dependent nature renders it inadequate in certain therapy contexts such as hypoxic tumors. As an alternative to PDT, oxygen free mechanisms to generate cytotoxic reactive oxygen species (ROS) by visible light cleavable photocages are in demand. Here, we report the detailed mechanisms by which the small molecule blebbistatin acts as a one-photon blue light-gated or two-photon near-infrared light-gated photocage to directly release a hydroxyl radical (•OH) in the absence of oxygen. By using femtosecond transient absorption spectroscopy and chemoselective ROS fluorescent probes, we analyze the dynamics and fate of blebbistatin during photolysis under blue light. Water-dependent photochemistry reveals a critical process of water-assisted protonation and excited state intramolecular proton transfer (ESIPT) that drives the formation of short-lived intermediates, which surprisingly culminates in the release of •OH but not superoxide or singlet oxygen from blebbistatin. CASPT2//CASSCF calculations confirm that hydrogen bonding between water and blebbistatin underpins this process. We further determine that blue light enables blebbistatin to induce mitochondria-dependent apoptosis, an attribute conducive to PACT development. Our work demonstrates blebbistatin as a controllable photocage for •OH generation and provides insight into the potential development of novel PACT agents

HKOCl‑2 Series of Green BODIPY-Based Fluorescent Probes for Hypochlorous Acid Detection and Imaging in Live Cells

A <b>HKOCl-2</b> series of new fluorescent probes for hypochlorous acid (HOCl) detection in live cells is reported. The probes exhibit excellent selectivity, sensitivity, and chemostability toward HOCl. In particular, <b>HKOCl-2b</b> rapidly and selectively detects endogenous HOCl in both human and mouse macrophages. These probes could therefore serve as promising discovery tools to help elucidate biological functions of HOCl

sj-tif-4-onc-10.1177_11795549221109511 – Supplemental material for GRHL2 Expression Functions in Breast Cancer Aggressiveness and Could Serve as Prognostic and Diagnostic Biomarker for Breast Cancer

Supplemental material, sj-tif-4-onc-10.1177_11795549221109511 for GRHL2 Expression Functions in Breast Cancer Aggressiveness and Could Serve as Prognostic and Diagnostic Biomarker for Breast Cancer by Xiaoyu Bai, Yue Li, Yanlei Li, Fan Li, Na Che, Chunsheng Ni, Nan Zhao, Xiulan Zhao and Tieju Liu in Clinical Medicine Insights: Oncology</p

Additional file 2 of High expression of eIF4E is associated with tumor macrophage infiltration and leads to poor prognosis in breast cancer

Additional file 2: Figure S2. eIF4E co-expression genes in BRCA (LinkedOmics). (A) The global eIF4E highly correlated genes identified by Pearson test in BRCA cohort. (B-C) Heat maps showing top 50 genes positively and negatively correlated with eIF4E in BRCA. Red indicates positively correlated genes and blue indicates negatively correlated genes. (D-E) Significantly enriched GO annotations and KEGG pathways of eIF4E in BRCA cohort. (F)Bar chart of Biological process categories, Cellular component categories and Molecular function categories

Additional file 8 of High expression of eIF4E is associated with tumor macrophage infiltration and leads to poor prognosis in breast cancer

Additional file 8: Table S5. eIF4E co-expression of PPI network in breast

sj-jpg-3-onc-10.1177_11795549221109511 – Supplemental material for GRHL2 Expression Functions in Breast Cancer Aggressiveness and Could Serve as Prognostic and Diagnostic Biomarker for Breast Cancer

Supplemental material, sj-jpg-3-onc-10.1177_11795549221109511 for GRHL2 Expression Functions in Breast Cancer Aggressiveness and Could Serve as Prognostic and Diagnostic Biomarker for Breast Cancer by Xiaoyu Bai, Yue Li, Yanlei Li, Fan Li, Na Che, Chunsheng Ni, Nan Zhao, Xiulan Zhao and Tieju Liu in Clinical Medicine Insights: Oncology</p

sj-docx-5-onc-10.1177_11795549221109511 – Supplemental material for GRHL2 Expression Functions in Breast Cancer Aggressiveness and Could Serve as Prognostic and Diagnostic Biomarker for Breast Cancer

Supplemental material, sj-docx-5-onc-10.1177_11795549221109511 for GRHL2 Expression Functions in Breast Cancer Aggressiveness and Could Serve as Prognostic and Diagnostic Biomarker for Breast Cancer by Xiaoyu Bai, Yue Li, Yanlei Li, Fan Li, Na Che, Chunsheng Ni, Nan Zhao, Xiulan Zhao and Tieju Liu in Clinical Medicine Insights: Oncology</p

Additional file 5 of High expression of eIF4E is associated with tumor macrophage infiltration and leads to poor prognosis in breast cancer

Additional file 5: Table S2. eIF4E co expressed differential genes

Additional file 1 of High expression of eIF4E is associated with tumor macrophage infiltration and leads to poor prognosis in breast cancer

Additional file 1: Figure S1. Correlation between eIF4E and prognosis of various types of cancer Correlation between eIF4E and prognosis of various types of cancer in the GEPIA

Additional file 4 of High expression of eIF4E is associated with tumor macrophage infiltration and leads to poor prognosis in breast cancer

Additional file 4: Table S1. Geo database data set GSE109169 differentially expressed genes