8 research outputs found
The difference in cytosolic esterase-induced fluorescence with CMFDA between resting and activated human platelets.
<p>The activity of cytosolic esterase-induced fluorescence reaction is visualized with CMFDA. The fluorescence of resting platelets was clearly visible in laser confocal microscopy, whereas activated platelets release no or little fluorescence. A, C: Resting platelets; B, D: Activated platelets (activated by 100μM ADP); A, B: green fluorescence; C, D: bright field.</p
CD62P expression percentage on membrane of platelets during 7-day storage at 22± 2°C.
<p>A: Mouse IgG1 κ Iso control. B: platelets stored for 1 day. C: platelets stored for 3 days. D: platelets stored for 5 days. E: platelets stored for 7 days.</p
The change of cytosolic esterase-induced fluorescence activity in platelets during 7-day storage at 22 ± 2°C.
<p>The activity of cytosolic esterase-induced fluorescence in platelets is measured with CMFDA. *<i>P</i> <0.05 as compared with platelets stored for 1 day.</p
Aggregation curves of platelets during 7-day storage at 22 ± 2°C.
<p>A: platelets stored for 1 day. B: platelets stored for 3 days. C: platelets stored for 5 days. D: platelets stored for 7 days.</p
- Changes of CEIFI, aggregation activity, HSR, CD62P expression and PS externalization in platelets during 7-day storage at 22 ± 2°C.
<p>Data are the mean ± S.D. *<i>P</i> <0.05 as compared with platelets stored for 1 day.</p
PS externalization of platelets during 7-day storage at 22 ± 2°C.
<p>A: platelets stored for 1 day. B: platelets stored for 3 days. C: platelets stored for 5 days. D: platelets stored for 7 days.</p
Correlation of platelet CEIFI with functional parameters of platelets during 7-day storage.
<p>A: Correlation to the ADP-induced aggregation activity, r = 0.9813. B: Correlation to the HSR activity, r = 0.9848. C: Correlation to the expression percentage of CD62P on platelet membrane, r = -0.9945. D: Correlation to the PS externalization percentage, r = -0.9847.</p
HSR curves of platelets during 7-day storage at 22± 2°C.
<p>A: platelets stored for 1 day. B: platelets stored for 3 days. C: platelets stored for 5 days. D: platelets stored for 7 days.</p