Reduction of egg-laying resulting from RNAi effects.

<p>(A) number of eggs laid by ds-<i>rab11</i> treated females and males (B) number of eggs laid by normal virgin females mating with ds-<i>noa</i> treated males. ds-<i>egfp</i> treatment group was used as control. NS stands for that there was no significant difference at p<0.05 level.</p

Knockdown of <i>noa</i> (A), <i>rab11</i> (B), <i>v-ATPase-D</i> (C) and <i>rpl19</i> (D) expression by ingestion of dsRNA.

<p>The samples of each group were collected on day0, day1, day2, day4 and day7. ds-<i>egfp</i> was used as control. All the experiments were triplicated. Asterisk stands for significant difference of relative expression. (p<0.05, Duncan's test).</p

Relative expression of <i>noa</i> in specific tissues.

<p>(A) Expression of <i>noa</i> after feeding bacteria expressing ds-<i>noa</i>. (B) Expression of <i>noa</i> after feeding ds-<i>noa</i>. N: nervous system; F: fatbody; T: testes; O: ovary; W: whole insect. <i>egfp</i> was used as control. All the experiments were triplicated. Asterisk stands for significant difference of relative expression. (p<0.05, Duncan's test).</p

dsRNA stability on artificial diet.

<p>dsRNA on artificial was extracted and examined by electrophoresis for integrity every one hour.</p

Relative expression of <i>rab11</i> in specific tissues.

<p>(A) Expression of <i>rab11</i> after feeding bacteria expressing ds-<i>rab11</i>. (B) Expression of <i>rab11</i> after feeding ds-<i>rab11</i>. M: midgut; T: testes; O: ovary; W: whole insect. <i>egfp</i> was used as control. All the experiments were triplicated. Asterisk stands for significant difference of relative expression. (p<0.05, Duncan's test).</p

Mortality rate of <i>rab11</i>.

<p>Mortality rate of adult flies in dsRNA treatment and bacteria treatment in 14 days bioassay was caculated. Asterisk stands for significant difference of relative expression. (p<0.05, Duncan's test).</p

Knockdown of <i>noa</i> (A), <i>rab11</i> (B), <i>v-ATPase-D</i> (C) and <i>rpl19</i> (D) expression by ingestion of bacteria expressing dsRNA.

<p>The samples of each group were collected on day0, day1, day2, day4 and day7. Bacteria expressing ds-<i>egfp</i> was used as control. All the experiments were triplicated. Asterisk stands for significant difference of relative expression. (p<0.05, Duncan's test).</p

PCR primers used for RT-PCR and qRT-PCR analyses.

<p>Primers starting with a D were degenerated primers to get the partial CDS of each gene. Primers starting with an S were used to get PCR fragment for L4440 plasmid construction. Primers starting with a Q were designed for qRT-PCR. The underlined part indicates enzyme restrict site.</p

Ingestion and accumulation of bacteria in the midgut of <i>Bactrocera dorsalis</i>.

<p>(A) The midgut from the adult fed on FD&C blue colored bacteria for 3 days. (B) Blue color accumulated in the abdomen of adult fed by FD&C blue for 3 days. Scale bars: 0.5 mm.</p

Relative expression of <i>rpl19</i> in specific tissues.

<p>(A) Expression of <i>rpl19</i> after feeding bacteria expressing ds-<i>rpl19</i>. (B) Expression of <i>rpl19</i> after feeding ds-<i>rpl19</i>. M: midgut; W: whole insect. <i>egfp</i> was used as control. All the experiments were triplicated. Asterisk stands for significant difference of relative expression. (p<0.05, Duncan's test).</p