DataSheet1_Enhancing osteogenesis and angiogenesis functions for Ti-24Nb-4Zr-8Sn scaffolds with methacrylated gelatin and deferoxamine.docx

Repair of large bone defects remains challenge for orthopedic clinical treatment. Porous titanium alloys have been widely fabricated by the additive manufacturing, which possess the elastic modulus close to that of human cortical bone, good osteoconductivity and osteointegration. However, insufficient bone regeneration and vascularization inside the porous titanium scaffolds severely limit their capability for repair of large-size bone defects. Therefore, it is crucially important to improve the osteogenic function and vascularization of the titanium scaffolds. Herein, methacrylated gelatin (GelMA) were incorporated with the porous Ti-24Nb-4Zr-8Sn (Ti2448) scaffolds prepared by the electron beam melting (EBM) method (Ti2448-GelMA). Besides, the deferoxamine (DFO) as an angiogenic agent was doped into the Ti2448-GelMA scaffold (Ti2448-GelMA/DFO), in order to promote vascularization. The results indicate that GelMA can fully infiltrate into the pores of Ti2448 scaffolds with porous cross-linked network (average pore size: 120.2 ± 25.1 μm). Ti2448-GelMA scaffolds facilitated the differentiation of MC3T3-E1 cells by promoting the ALP expression and mineralization, with the amount of calcium contents ∼2.5 times at day 14, compared with the Ti2448 scaffolds. Impressively, the number of vascular meshes for the Ti2448-GelMA/DFO group (∼7.2/mm2) was significantly higher than the control group (∼5.3/mm2) after cultivation for 9 h, demonstrating the excellent angiogenesis ability. The Ti2448-GelMA/DFO scaffolds also exhibited sustained release of DFO, with a cumulative release of 82.3% after 28 days. Therefore, Ti2448-GelMA/DFO scaffolds likely provide a new strategy to improve the osteogenesis and angiogenesis for repair of large bone defects.</p

Additional file 2: Figure S1. of miR-589-5p inhibits MAP3K8 and suppresses CD90+ cancer stem cells in hepatocellular carcinoma

CD90 is predominantly expressed in a small population in HCC cell lines. Figure S2. CD90+ HCC cells possess CSC characteristics. Figure S3. Overexpression of miR-589-5p has no impact on the regulation of MAP3K8 and stemness in CD90- HCC cells. Figure S4. Suppression of miR-589-5p fails to alter the CD90+ population in HCC cells. Figure S5. CD90- tumor xenograft contains CD90+ cells (DOCX 16 kb

Additional file 1: Table S1. of miR-589-5p inhibits MAP3K8 and suppresses CD90+ cancer stem cells in hepatocellular carcinoma

Antibodies used in this study. Table S2. The primers have been used for quantitative real-time PCR. Table S3. All differentially expressed microRNAs in MHCC97H and MHCC97L CD90+ cells. Table S4. The relationship of CD90 and miR-589-5p expression to clinical parameters in human HCC (n=40). (DOCX 21 kb

Origin of Dirac Cones in SiC Silagraphene: A Combined Density Functional and Tight-Binding Study

The formation of Dirac cones in electronic band structures via isomorphous transformation is demonstrated in 2D planar SiC sheets. Our combined density functional and tight-binding calculations show that 2D SiC featuring C–C and Si–Si atom pairs possesses Dirac cones (DCs), whereas an alternative arrangement of C and Si leads to a finite band gap. The origin of Dirac points is attributed to bare interactions between Si–Si bonding states (valence bands, VBs) and C–C antibonding states (conduction bands, CBs), while the VB–CB coupling opens up band gaps elsewhere. A mechanism of atom pair coupling is proposed, and the conditions required for DC formation are discussed, enabling one to design a class of 2D binary Dirac fermion systems on the basis of DF calculations solely for pure and alternative binary structures

Additional file 2: of Circular RNA IARS (circ-IARS) secreted by pancreatic cancer cells and located within exosomes regulates endothelial monolayer permeability to promote tumor metastasis

Table S2 siRNA. (DOCX 13 kb

Additional file 1: of Circular RNA IARS (circ-IARS) secreted by pancreatic cancer cells and located within exosomes regulates endothelial monolayer permeability to promote tumor metastasis

Table S1 Sequences of Primers. (DOCX 19 kb

Isomerism in Au–Ag Alloy Nanoclusters: Structure Determination and Enantioseparation of [Au₉Ag₁₂(SR)₄(dppm)₆X₆]³⁺

Revealing structural isomerism in a nanocluster remains significant but challenging. Herein, we have obtained a pair of structural isomers, [Au₉­Ag₁₂­(SR)₄­(dppm)₆­X₆]³⁺-C and [Au₉­Ag₁₂­(SR)₄­(dppm)₆­X₆]³⁺-Ac [dppm = bis­(diphenyphosphino)­methane; HSR = 1-adamantanethiol/<i>tert</i>-butylmercaptan; X = Br/Cl; C stands for one of the structural isomers being chiral; Ac stands for another being achiral], that show different structures as well as different chiralities. These structures are determined by single-crystal X-ray diffraction and further confirmed by high-resolution electrospray ionization mass spectrometry. On the basis of the isomeric structures, the most important finding is the different arrangements of the Au₅Ag₈@Au₄ metal core, leading to changes in the overall shape of the cluster, which is responsible for structural isomerism. Meanwhile, the two enantiomers of [Au₉­Ag₁₂­(SR)₄­(dppm)₆­X₆]³⁺-C are separated by high-performance liquid chromatography. Our work will contribute to a deeper understanding of the structural isomerism in noble-metal nanoclusters and enrich the chiral nanocluster