Table1_Genome-Wide Characterization and Analysis of the bHLH Transcription Factor Family in Suaeda aralocaspica, an Annual Halophyte With Single-Cell C4 Anatomy.docx

Basic helix-loop-helix (bHLH) transcription factors play important roles in plant growth, development, metabolism, hormone signaling pathways, and responses to abiotic stresses. However, comprehensive genomic and functional analyses of bHLH genes have not yet been reported in desert euhalophytes. Suaeda aralocaspica, an annual C4 halophyte without Kranz anatomy, presents high photosynthetic efficiency in harsh natural habitats and is an ideal plant for identifying transcription factors involved in stress resistance. In this study, 83 bHLH genes in S. aralocaspica were identified and categorized into 21 subfamilies based on conserved motifs, gene structures, and phylogenetic analysis. Functional annotation enrichment revealed that the majority of SabHLHs were enriched in Gene Ontology (GO) terms and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways involved in the response to stress conditions, as transcription factors. A number of cis-acting elements related to plant hormones and stress responses were also predicted in the promoter regions of SabHLHs, which were confirmed by expression analysis under various abiotic stress conditions (NaCl, mannitol, low temperature, ABA, GA3, MeJA, and SA); most were involved in tolerance to drought and salinity. SabHLH169 (076) protein localized in the nucleus was involved in transcriptional activity, and gene expression could be affected by different light qualities. This study is the first comprehensive analysis of the bHLH gene family in S. aralocaspica. These data will facilitate further characterization of their molecular functions in the adaptation of desert plants to abiotic stress.</p

High-Performance, Room-Temperature, and No-Humidity-Impact Ammonia Sensor Based on Heterogeneous Nickel Oxide and Zinc Oxide Nanocrystals

NiO nanocones decorated with ZnO nanothorns on NiO foil substrates are shown to be an ammonia sensor with excellent comprehensive performance, which could, in real-time, detect and monitor NH₃ in the surrounding environment. Gas-sensing measurements indicate that assembling nanocones decorated with nanothorns on NiO foil substrate is an effective strategy for simultaneously promoting the stability, reproducibility, and sensitivity of the sensor, because the NiO foil substrate as a whole can quickly and stably transfer electrons between the gas molecules and the sensing materials and the large specific surface area of both nanocones and nanothorns provide good accessibility of the gas molecules to the sensing materials. Moreover, p-type NiO, with majority charge carriers of holes, has higher binding affinity for the electron-donating ammonia, resulting in a significant increase in selectivity toward NH₃ over other organic gases. Compared with the NiO nanowires and pure NiO nanocones, the heterogeneous NiO nanocones/ZnO nanothorns exhibit less dependence on the temperature and humidity in response/recovery speed and sensitivity of sensing NH₃. Our investigation indicates that two factors are responsible for reducing the dependence on the gas sensing characteristics under various environmental conditions. One is that the n-type ZnO nanothorns growing on the surface of nanocones, with majority charge carriers of electrons, speed up adsorption and desorption of gas molecules. The other is that the abundant cone-shaped and thornlike superstructures on the substrate are favorable for constructing a hydrophobic surface, which prevents the gas sensing material from being wetted

Image1_Genome-Wide Characterization and Analysis of the bHLH Transcription Factor Family in Suaeda aralocaspica, an Annual Halophyte With Single-Cell C4 Anatomy.TIF

Basic helix-loop-helix (bHLH) transcription factors play important roles in plant growth, development, metabolism, hormone signaling pathways, and responses to abiotic stresses. However, comprehensive genomic and functional analyses of bHLH genes have not yet been reported in desert euhalophytes. Suaeda aralocaspica, an annual C4 halophyte without Kranz anatomy, presents high photosynthetic efficiency in harsh natural habitats and is an ideal plant for identifying transcription factors involved in stress resistance. In this study, 83 bHLH genes in S. aralocaspica were identified and categorized into 21 subfamilies based on conserved motifs, gene structures, and phylogenetic analysis. Functional annotation enrichment revealed that the majority of SabHLHs were enriched in Gene Ontology (GO) terms and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways involved in the response to stress conditions, as transcription factors. A number of cis-acting elements related to plant hormones and stress responses were also predicted in the promoter regions of SabHLHs, which were confirmed by expression analysis under various abiotic stress conditions (NaCl, mannitol, low temperature, ABA, GA3, MeJA, and SA); most were involved in tolerance to drought and salinity. SabHLH169 (076) protein localized in the nucleus was involved in transcriptional activity, and gene expression could be affected by different light qualities. This study is the first comprehensive analysis of the bHLH gene family in S. aralocaspica. These data will facilitate further characterization of their molecular functions in the adaptation of desert plants to abiotic stress.</p

DataSheet_1_Nitrate nitrogen enhances the efficiency of photoprotection in Leymus chinensis under drought stress.docx

IntroductionGlobal climate change exerts a significant impact on the nitrogen supply and photosynthesis ability in land-based plants. The photosynthetic capacity of dominant grassland species is important if we are to understand carbon cycling under climate change. Drought stress is one of the major factors limiting plant photosynthesis, and nitrogen (N) is an essential nutrient involved in the photosynthetic activity of leaves. The regulatory mechanisms responsible for the effects of ammonium (NH4+) and nitrate (NO3-) on the drought-induced photoinhibition of photosystem II (PSII) in plants have yet to be fully elucidated. Therefore, there is a significant need to gain a better understanding of the role of electron transport in the photoinhibition of PSII.MethodsIn the present study, we conducted experiments with normal watering (LD), severe drought (MD), and extreme drought (HD) treatments, along with no nitrogen (N0), ammonium (NH4), nitrate (NO3), and mixed nitrogen (NH4NO3) treatments. We analyzed pigment accumulation, reactive oxygen species (ROS) accumulation, photosynthetic enzyme activity, photosystem activity, electron transport, and O-J-I-P kinetics.ResultsAnalysis showed that increased nitrate application significantly increased the leaf chlorophyll content per unit area (Chlarea) and nitrogen content per unit area (Narea) (p3-treated plants than in N0 plants, and there was no significant difference in photosynthetic enzyme activity between plants treated with NO3 and NH4NO3. Under drought stress, the maximum photochemical efficiency of PSII (Fv/Fm), PSII electron transport rate (ETR), and effective quantum yield of PSII (φPSII) were significant higher in NO3-treated plants (p3-treated plants.DiscussionThese results suggest that drought stress hindered the formation of NADPH and ATP in N0 and NH4-treated L. chinensis plants, thus damaging the donor side of the PSII oxygen-evolving complex (OEC). After applying nitrate, higher photosynthetic enzyme and antioxidant enzyme activity not only protected PSII from photodamage under drought stress but also reduced the rate of damage in PSII during the growth of L. chinensis growth under drought stress.</p

Noninvasive Multiplexed Analysis of Bladder Cancer-Derived Urine Exosomes via Janus Magnetic Microspheres

Bladder cancer greatly endangers human health, and its early diagnosis is of vital importance. Exosomes, which contain proteins and nucleic acids related to their source cells, are expected to be an emerging biomarker for bladder cancer detection. Here, we propose a novel system for multiplexed analysis of bladder cancer-derived urine exosomes based on Janus magnetic microspheres as barcoded microcarriers. The microcarriers are constructed by droplet-templated coassembly of colloidal silica nanoparticles and magnetic nanoparticles under a magnetic field. The microcarriers possess one hemisphere with structural color and the other hemisphere with magneto-responsiveness. Benefiting from the unique structure, these Janus microcarriers could serve as barcodes and could move controllably in a sample solution, thus realizing the multiplex detection of exosomes with high sensitivity. Notably, the present platform is noninvasive since a urine specimen, as an ideal source of bladder cancer-derived exosomes, is employed as the sample solution. This feature, together with the good sensitivity, specificity, low sample consumption, and easy operation, indicates the great potential of the platform for bladder cancer diagnosis in clinical applications

The phenotypic characteristics of the study population.

<p>Data are presented in mean ±s.d. BMI: body mass index; FPG: fasting plasma glucose; CAD: coronary artery disease; T2DM: type 2 diabetes. The fasting plasma glucose values were after intervention. Independent t test was used to evaluate age and BMI; Mann-Whitney U test was used to compare the difference of T2DM duration and FBG between the two groups.</p

sj-docx-1-tct-10.1177_15330338231184995 - Supplemental material for Plasma Thioredoxin Reductase as a Potential Biomarker for Gynecologic Cancer

Supplemental material, sj-docx-1-tct-10.1177_15330338231184995 for Plasma Thioredoxin Reductase as a Potential Biomarker for Gynecologic Cancer by Yinxing Zhu, MS, Yixuan Hu, MS, Junfeng Shi, MD, Xiaowei Wei, MD, Yaqi Song, MD, Cuiju Tang, MD, PhD, and Wenwen Zhang, MD, PhD in Technology in Cancer Research & Treatment</p

PCSK1 SNPs Information.

<p>Position information was got from Hapmap. HW <i>P</i> value: <i>P</i> values of the Chi square test for Hardy–Weinberg equilibrium.</p

Association of the frequencies of common haplotypes with CAD.

<p>CAD: coronary artery disease. Chi square test was used for statistical analysis.</p