3 research outputs found

    Additional file 1 of HepaClear, a blood-based panel combining novel methylated CpG sites and protein markers, for the detection of early-stage hepatocellular carcinoma

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    Additional file 1. Figure S1. GO and KEGG pathway enrichment analysis of differentially methylated CpG sites. Figure S2. Performance of 32 candidate CpG sites identified from methylome profiling data. Figure S3. Methylation levels of 10 candidate CpG sites in two HCC cell lines and leukocytes using qMSP. Figure S4. Methylation/Protein levels and diagnostic performance of eight candidate markers in plasma pilot test set. Figure S5. Amplification curves of cg14263942, cg12701184 and cg14570307 within quadruplex assays. Table S1. Demographic and clinical characteristics of HCC patients for biomarker screening and tissue validation. Table S2. Top-1000 hypermethylation markers screened from genome-wide methylation profiling. Table S3. List of 132 hypermethylation markers with p 0.3 and βnormal 0.85 and Youden Index (YI) ≥ 0.8. Table S5. Characteristics of study participants in plasma pilot study. Table S6. Performance of different biomarker combinations in 150 plasma samples. Table S7. Limit of detection (LOD) of three methylation markers in HepaClear panel. Table S8. List of primers and probes for Taqman qMSP

    Genome Mining Reveals a UbiA-Type Prenyltransferase Access to Farnesylation of Diketopiperazines

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    UbiA-type prenyltransferases (PTases) are significant enzymes that lead to structurally diverse meroterpenoids. Herein, we report the identification and characterization of an undescribed UbiA-type PTase, FtaB, that is responsible for the farnesylation of indole-containing diketopiperazines (DKPs) through genome mining. Heterologous expression of the fta gene cluster and non-native pathways result in the production of a series of new C2-farnesylated DKPs. This study broadens the reaction scope of UbiA-type PTases and expands the chemical diversity of meroterpenoids

    AuFe<sub>3</sub>@Pd/γ-Fe<sub>2</sub>O<sub>3</sub> Nanosheets as an In Situ Regenerable and Highly Efficient Hydrogenation Catalyst

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    Heterogenous Pd catalysts play a pivotal role in the chemical industry; however, it is plagued by S2– or other strong adsorbates inducing surface poisoning long term. Herein, we report the development of AuFe3@Pd/γ-Fe2O3 nanosheets (NSs) as an in situ regenerable and highly active hydrogenation catalyst. Upon poisoning, the Pd monolayer sites could be fully and oxidatively regenerated under ambient conditions, which is initiated by •OH radicals from surface defect/FeTetra vacancy-rich γ-Fe2O3 NSs via the Fenton-like pathway. Both experimental and theoretical analyses demonstrate that for the electronic and geometric effect, the 2–3 nm AuFe3 intermetallic nanocluster core promotes the adsorption of reactant onto Pd sites; in addition, it lowers Pd’s affinity for •OH radicals to enhance their stability during oxidative regeneration. When packed into a quartz sand fixed-bed catalyst column, the AuFe3@Pd/γ-Fe2O3 NSs are highly active in hydrogenating the carbon–halogen bond, which comprises a crucial step for the removal of micropollutants in drinking water and recovery of resources from heavily polluted wastewater, and withstand ten rounds of regeneration. By maximizing the use of ultrathin metal oxide NSs and intermetallic nanocluster and monolayer Pd, the current study demonstrates a comprehensive strategy for developing sustainable Pd catalysts for liquid catalysis
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