26 research outputs found

    Ligands used for protein-ligand interaction analysis.

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    <p>The IUPAC name, structure, molecular weight and PubChem CID is provided for the ligands.</p

    Effect of dietary flavones on EZH2 catalytic activity and EZH2 and H3K27me3 protein expression.

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    <p>(A) Dose-dependent inhibition of EZH2 catalytic activity measured through H3K27me3 with 10- μM and 20-μM concentrations of DZNep, Apigenin, Chrysin and Luteolin. The results are the mean of 3–4 determinations and were analyzed with a one way ANOVA, bars ± SD. **P<0.001. (B) Inhibition of EZH2 and H3K27me3 protein expression after treatment with 20 μM DZNep, Apigenin, Chrysin and Luteolin for 48 h. Anti-β-actin and anti-histone H3 were used as loading controls.</p

    Interaction of dietary flavones with AT-rich and GC-rich DNA sequences.

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    <p>(A) UV-Vis spectra of AT-rich and GC-rich DNA sequences, with 5-aza-2’deoxycytidine, (B) Apigenin, (C) Chrysin, and (D) Luteolin. 100 bp highly GC-rich oligos mimicking CpG islands ranging from -183 to-83 and AT-rich oligos from the -486 to-386 region of the GSTP1 promoter were synthesized and used as a DNA substrate (2.5 mM) for binding with flavones at a 0.5 mM concentration. Absorption spectra of solutions were recorded from 220 nm to 500 nm using Nanodrop. The experiment was repeated three times with similar results. Details are described in the Materials and Methods section.</p

    In vitro chemotaxis assay.

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    <p>10 ng/ml rMuSDF-1 induced the migration of mouse BMSCs by 96%, and 100 ng/ml rMuSDF-1 by 254%. The effect was reduced after treated with AMD3100. Values are the mean and SD results from 4 independent experiments (*P<0.05; **P<0.001).</p

    Immunohistochemical staining for SDF-1 in groups TBI/fracture and fracture only on day 2 after surgery.

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    <p>The expression of SDF-1 was observed in both groups. High expression of SDF-1 protein was detected at the surrounding tissue of the damaged bone in the TBI/fracture group.</p