Additional file 4 of Association between anemia and serum Klotho in middle-aged and older adults

Additional file 4. Subgroup analyses for the association between anemia and quartiles of S-Klotho (pg/mL)among the study participants, weighted

Additional file 2 of Association between anemia and serum Klotho in middle-aged and older adults

Additional file 2. Relationship between anemia and S-Klotho (pg/mL) in 3 models, weighted

Additional file 1 of Association between anemia and serum Klotho in middle-aged and older adults

Additional file 1. Baseline characteristics of the study participants from NHANES 2007-2016, weighted

Patterning of Layer-by-Layer Assembled Organic−Inorganic Hybrid Films: Imprinting versus Lift-Off

Layer-by-layer (LbL) assembled organic−inorganic poly(acrylic acid) (PAA)/poly(allylamine hydrochloride) (PAH)/Au nanoparticle hybrid films are patterned by using Norland Optical Adhesive 63 (NOA 63) polymer molds. Depending on the rigidity of the hybrid films, their patterning can be realized by a room-temperature imprinting or lift-off process. For [(PAA/PAH)1-(Au nanoparticle/PAH)3]∗10 and [(PAA/PAH)3-(Au nanoparticle/PAH)3]∗5 films which have a low content of Au nanoparticles, the films can be imprinted at room temperature to form patterned films with large areas because of the compressibility and fluidity of the films under high pressure. The Au nanoparticle/PAH films, which have an extremely high content of Au nanoparticles and are fragile, can be patterned by a lift-off process during which the film contacted with the NOA 63 mold was peeled off because of the strong adhesion between the film and the mold and the fragility of the film. The complementary room-temperature imprinting and lift-off methods with polymer NOA 63 molds provide facile and general ways to pattern LbL assembled organic−inorganic films with various film compositions

Additional file 3 of Association between anemia and serum Klotho in middle-aged and older adults

Additional file 3. Threshold effects of S-Klotho (per 100 pg/mL) for anemia were analyzed in NHANES2007-2016 using a two-piece regression model

Validation of Reference Genes for Real-Time Quantitative PCR (qPCR) Analysis of <i>Avibacterium paragallinarum</i> - Fig 1

Range of Cq values of the nine candidate reference genes across all samples of A. paragallinarum serovars A, B, and C. Boxes and whiskers represent interquartile ranges and confidence intervals. Bars inside boxes indicate median values. Hollow circles show outliers (5th/95th percentile) respectively.</p

Candidate reference genes ranked by different methods in serovar A.

<p>Candidate reference genes ranked by different methods in serovar A.</p

Gene expression stability rankings for different growth phase in <i>A</i>. <i>paragallinarum</i> serovar C analyzed by geNorm.

<p>Gene expression stability rankings for different growth phase in <i>A</i>. <i>paragallinarum</i> serovar C analyzed by geNorm.</p

Gene expression stability assessed by the comparative ΔCT method.

<p>Gene expression stability assessed by the comparative ΔCT method.</p

50S ribosomal protein L33 expression analysis at different time points in stationary phase in cultures of serovar B of <i>A</i>. <i>paragallinarum</i>.

<p>Different colored columns show the relative quantification of 50S ribosomal protein L33 when normalized against reference genes at three sampling time points. <i>RecN</i> and <i>16S rRNA</i> were the two highest ranked genes, <i>gyrA</i> and <i>atpD</i> were the two highly ranked genes for cultures of serovar B of <i>A</i>. <i>paragallinarum</i> in stationary phase and <i>sodA</i> was a poorly ranked gene.</p