Data_Sheet_1_The role of patient volunteers in Fangcang Shelter Hospital during the Omicron wave of COVID-19 pandemic in Shanghai, China: a qualitative study.DOCX

ObjectiveDuring the Omicron wave of the COVID-19 pandemic in Shanghai, Fangcang Shelter Hospital (FSH) served as the major way in patient quarantine. Many COVID patients served as volunteers in FSH providing a lot of assistance for the medical workers and other COVID patients. The aim of this study was to explore the experiences of patient volunteers in FSH. It helps health professionals better understand their motivational incentives and barriers in their volunteer work, and improves recruiting and managing volunteers in subsequent public health emergencies.MethodsThis is a qualitative study using semi-structured interviews. Thirteen patient volunteers working in an FSH in Shanghai were included. Thematic analysis was applied to data analysis.ResultsFour themes and nine subthemes were identified. The wishes to give back to society and the responsibility of politics and religion were the main reasons for the patients to serve as volunteers in FSH. The patient volunteers served as the bridge to reduce the communication barriers between other patients and healthcare professionals. They also provided support in supply distribution and psychological counseling. They viewed voluntary work as a usual task and tried to solve the barriers in their work. In turn, the voluntary work brought them benefits in mental and physical health, as well as another chance for growth.ConclusionWorking as volunteers in FSHs not only brought personal benefits to the COVID patients but also fulfilled the needs of the healthcare system during public health emergencies. The mode of mutual help between patients could be taken as an example in other public health emergencies.</p

Table_1_Overexpression of PKMYT1 associated with poor prognosis and immune infiltration may serve as a target in triple-negative breast cancer.xlsx

Breast cancer (BC) is one of the most common malignancies among women worldwide. It is necessary to search for improvement in diagnosis and treatment methods to improve the prognosis. Protein kinase, membrane associated tyrosine/threonine 1 (PKMYT1), a member of the Wee family of protein kinases, has been studied in some tumors except BC. This study has explored that PKMYT1 functional role by bioinformatics methods combined with local clinical samples and experiments. Comprehensive analysis showed that PKMYT1 expression was higher in BC tissues, especially in advanced patients than that in normal breast tissues. The expression of PKMYT1 was an independent determinant for BC patients’ prognosis when combined with the clinical features. In addition, based on multi-omics analysis, we found that the PKMYT1 expression was closely relevant to several oncogenic or tumor suppressor gene variants. The analysis of single-cell sequencing indicated that PKMYT1 expression was upregulated in triple-negative breast cancer (TNBC), consistent with the results of bulk RNA-sequencing. High PKMYT1 expression was correlated with a poor prognosis. Functional enrichment analysis revealed that PKMYT1 expression was associated with cell cycle-related, DNA replication-related, and cancer-related pathways. Further research revealed that PKMYT1 expression was linked to immune cell infiltration in the tumor microenvironment. Additionally, loss-of-function experiments in vitro were performed to investigate the role of PKMYT1. TNBC cell lines’ proliferation, migration, and invasion were inhibited when PKMYT1 expression was knock-down. Besides, the down-regulation of PKMYT1 induced apoptosis in vitro. As a result, PKMYT1 might be a biomarker for prognosis and a therapeutic target for TNBC.</p

Additional file 4: Figure S4. of Breast cancer stem cell selectivity of synthetic nanomolar-active salinomycin analogs

Salinomycin treatment decreases the proportion of ALDH+ in a dose dependent manner. JIMT-1 cells were treated with salinomycin for 72 h at the indicated concentrations. The effect on the ALDH+ population was determined using flow cytometry. Data are represented as mean ± SEM for n = 3. (DOCX 38 kb

Additional file 7: Figure S7. of Breast cancer stem cell selectivity of synthetic nanomolar-active salinomycin analogs

Cell cycle effects of treating with 50 nM salinomycin or the analogs 2a-c for 72 h. (a) Cell cycle phase distribution evaluated using flow cytometry. (b) Representative Western blots used for densitometric scanning to obtain the data in (c) and (d). (c-d) Expression of cyclin A2 and p27, respectively. The columns in (c) and (d) show mean ± SEM for n = 6. * P < 0.05. SA: salinomycin, 2a: carbamate, 2b: acetate, 2c: carbonate and 3: C1-methyl ester. (JPG 737 kb

Highly Fluorescent Metal–Organic Frameworks Based on a Benzene-Cored Tetraphenylethene Derivative with the Ability To Detect 2,4,6-Trinitrophenol in Water

One tetracarboxylate functionalized benzene-cored tetraphenylethene derivative was designed as a linker for construction of aggregation-induced emission (AIE) active metal–organic frameworks (MOFs), which exhibit the distinct AIE behavior. Two two-dimensional MOFs materials, MOF-<b>1</b> and MOF-<b>2</b>, have been successfully synthesized by using the organic linker precursor. Photophysical studies reveal that both MOFs exhibit remarkable fluorescent properties with the absolute quantum yield as high as 43%. The high PL efficiency, good water stability, as well as the electron-donating nature of prepared MOF materials, allow us to explore their potentials in sensing applications for electron-deficient nitro explosives in aqueous media. Especially, water-stable MOF-<b>2</b> exhibits superior sensitivity and selectivity toward 2,4,6-trinitrophenol (TNP) over other selected nitro analytes, including nitromethane, 2,3-dimethyl-dinitrobutane, nitrobenzene, 2,4,6-trinitrotoluene, 2,6-dinitrotoluene, 2,4-dinitrotoluene, 1,3-dinitrobenzene, with the detection limit for TNP as low as 0.49 μM (∼110 ppb). In virtue of its excellent detection efficiency, applicability in water and reusability, MOF-<b>2</b> can be a promising fluorescent sensor material for the practical TNP sensing

Additional file 3: Figure S3. of Breast cancer stem cell selectivity of synthetic nanomolar-active salinomycin analogs

Representative cytograms of ALDH assay obtained using flow cytometry. JIMT-1 cells were treated with 50 nM salinomycin or salinomycin analogs for 72 h. SA: salinomycin, 2a: carbamate, 2b: acetate, 2c: carbonate and 3: C1-methyl ester. (DOCX 1678 kb

One-Dimensional Non-coplanar Nitrogen Chains in Manganese Tetranitride under High Pressure

Transition metal nitrides have great potential applications as incompressible and high energy density materials. Various polymeric nitrogen structures significantly affect their properties, contributing to their complex bonding modes and coordination conditions. Herein, we first report a new manganese polynitride MnN4 with bifacial trans–cis [N4]n chains by treating with high-pressure and high-temperature conditions in a diamond anvil cell. Our experiments reveal that MnN4 has a P-1 symmetry and could stabilize in the pressure range of 56–127 GPa. Detailed pressure–volume data and calculations of this phase indicate that MnN4 is a potential hard (255 GPa) and high energy density (2.97 kJ/g) material. The asymmetric interactions impel N1 and N4 atoms to hybridize to sp2–3, which causes distortions of [N4]n chains. This work discovers a new polynitride material, fills the gap for the study of manganese polynitride under high pressure, and offers some new insights into the formation of polymeric nitrogen structures

Confirmation of the Structural Phase Transitions in XeF₂ under High Pressure

Unique structures and properties will be introduced upon compression. To figure out the high pressure crystal structure of XeF₂, in situ synchrotron X-ray diffraction, Raman spectra, UV–vis absorption spectra, and theoretical calculations have been performed up to 86 GPa. The structural dispute between reported experimental and theoretical results is settled by this study. The experimental and theoretical Raman spectra results indicated that the ambient structure of XeF₂ (<i>I</i>4/<i>mmm</i>) transformed into a <i>Immm</i> structure at 28 GPa. Then this <i>Immm</i> structure transformed into <i>Pnma</i> structure at 59 GPa. The Rietveld refinement of the XRD results was in accordance with our Raman study. The optical absorption spectra revealed a reduction in the band gap as pressure increases. The reduction in the band gap decreases to 1.83 eV at 82 GPa while the color of the sample is getting dark. Our results provide a new insight into the high pressure behavior of noble gas compounds with the example of XeF₂

Pressure-Dependent Light Emission of Charged and Neutral Excitons in Monolayer MoSe₂

Tailoring the excitonic properties in two-dimensional monolayer transition metal dichalcogenides (TMDs) through strain engineering is an effective means to explore their potential applications in optoelectronics and nanoelectronics. Here we report pressure-tuned photon emission of trions and excitons in monolayer MoSe₂ via a diamond anvil cell (DAC) through photoluminescence measurements and theoretical calculations. Under quasi-hydrostatic compressive strain, our results show neutral (X⁰) and charged (X^–) exciton emission of monolayer MoSe₂ can be effectively tuned by alcohol mixture vs inert argon pressure transmitting media (PTM). During this process, X^– emission undergoes a continuous blue shift until reaching saturation, while X⁰ emission turns up splitting. The pressure-dependent charging effect observed in alcohol mixture PTM results in the increase of the X^– exciton component and facilitates the pressure-tuned emission of X^– excitons. This substantial tunability of X^– and X⁰ excitons in MoSe₂ can be extended to other 2D TMDs, which holds potential for developing strained and optical sensing devices

Coupling-Assisted Renormalization of Excitons and Vibrations in Compressed MoSe₂–WSe₂ Heterostructure

Vertical heterostructures (HSs) constructed with two-dimensional (2D) materials is expected to generate fascinating properties due to interlayer coupling between neighboring layers. However, interlayer coupling can be easily obscured by cross-contamination during transfer processes, rendering their experimental demonstration challenging. Here, we explore the coupling-assisted renormalization of excitons and vibrations in a mechanically fabricated MoSe₂–WSe₂ HS through high-pressure photoluminescence, Raman spectra, and density functional theory calculations. Accompanied by the interlayer coupling enhancement, the excitonic and vibrational renormalizations involving dimensionality and composition variations were achieved. A cycle of 2D–3D–2D excitonic evolution was disclosed and pressure-induced emergence of X^– exciton of MoSe₂ in HS was found reflecting the band structure transition in the MoSe₂–WSe₂ HS. The Raman spectra reveals that the coupled A₂″ vibrations of WSe₂ and MoSe₂ in HS was stiffened and out-of-plane A₁′ vibrations of WSe₂ and MoSe₂ in HS got coherent upon pressure modulation. This coupling-assisted renormalization in MoSe₂–WSe₂ HS can be extended to other 2D layered HSs, which indicates the possibility to design a flexible HS with controlled excitonic and vibrational system for light-emitting diodes, excitonic, and photovoltaic devices