The principle underlying CC (centrifuge column) and MB (magnetic bead) methods for DNA extraction.

(TIF)</p

PCR reaction details.

PCR reaction details.</p

Fig 2 -

(a) The amount of extracted DNA during repeated serial elutions among TIANGEN, NANOEAST and QIAGEN. (b) DNA electrophoresis of the related samples (Marker,TIANGEN, NANOEAST and QIAGEN).</p

Degree of linear fitting and correlation among the extracted DNA, cellulose, hemicellulose, lignin, AmorI, and CA in the adsorbents.

Degree of linear fitting and correlation among the extracted DNA, cellulose, hemicellulose, lignin, AmorI, and CA in the adsorbents.</p

Fig 7 -

(a) Comparison of the DNA extracting capacities of the current approaches, BP/DC with commercial protocols, and BP/DC with the new protocol. (b) A UV imager was used to compare DNA absorbed utilizing a single piece of PF-BA against blank controls. (c) DNA electrophoresis of the blanks of BP/DC with the new protocol (Marker; BP; DC). (d) The PCR products of negative control, TIANGEN, NANOEAST, QIAGEN and DC.</p

S5 Fig -

(a) The capacities of lysis buffer C (pH 4 to 10), and elution buffer Q (pH 9) for extracting DNA on DC, using the new protocol. (b). DNA electrophoresis of the related samples (Marker, *lyC4,5,6,7,8,9,10DC; *lyC and the number refers to lysis buffer C and the pH of lysis buffer C). (c) The OD260/OD280 (black) and OD260/OD230 (purple) purity ratios of the related samples. (TIF)</p

S2 Fig -

(a) The customized 7 mm hole puncher (HONGXUAN). (b). Representative contents of the assembled extraction spin column sets. (TIF)</p

S6 Fig -

(a) The capacities of lysis buffer N (pH 4 to 10) and elution buffer Q (pH 9) for extracting DNA on DC, using the new protocol. (b) DNA electrophoresis of the related samples (Marker, *lyN4,5,6,7,8,9,10DC; *lyC and the number refers to lysis buffer N and the pH of lysis buffer N). (c) The OD260/OD280 (black) and OD260/OD230 (purple) purity ratios of the related samples. (TIF)</p

Overview of protocols for the five current DNA extraction approaches.

(TIF)</p

pH of the serial buffer solutions and ethanol used in the existing DNA extraction protocols of current approaches.

pH of the serial buffer solutions and ethanol used in the existing DNA extraction protocols of current approaches.</p