Kaplan-Meier curve of progression-free survival (PFS) according to ribonucleotide reductase M1 (RRM1) expression.

<p>(A) PFS for all patients with negative or positive RRM1 expression. (B) PFS for patients receiving gemcitabine-based therapy. (C) PFS for patients receiving docetaxel-based therapy. (D) PFS for patients receiving vinorelbine-based therapy.</p

Kaplan-Meier curve of progression-free survival (PFS) according to chemotherapy regimen.

<p>(A) PFS for patients with RRM1-negative tumors. (B) PFS for patients with RRM1-positive tumors.</p

Table1_The Prognostic Model and Drug Sensitivity of LKB1-Mutant Lung Adenocarcinoma Based on Immune Landscape.DOCX

Background: Lung cancer is the most common cause of cancer-related deaths worldwide. LKB1-mutant lung adenocarcinoma (LUAD) is a unique subtype of this deadly cancer. LKB1 mutations cause functional changes in a variety of cell processes, including immune functions, that affect prognosis. To date, the potential role of immunity in the prognosis of LKB1-mutant LUAD is not well understood.Methods: We systematically analyzed immune-related genes in LUAD samples from The Cancer Genome Atlas (TCGA) database. ESTIMATE and CIBERSORT algorithms were used to explore the immune microenvironment. A prognostic risk model was constructed, and prognostic, immune function, drug sensitivity, and model specificity analyses were performed to identify the effectiveness of the model.Results: Our results showed that LKB1 mutations suppressed immune function in LUAD. A three-gene signature was constructed to stratify patients into two risk groups. The risk score was an independent predictor for overall survival (OS) in multivariate Cox regression analyses [hazard ratio (HR) > 1, p = 0.002]. Receiver operating characteristic (ROC) curve analyses confirmed that the risk score has better performance than clinicopathological characteristics. Functional analysis revealed that the immune status was different between the risk groups. ZM.447439 was an appropriate treatment for the high-risk group of patients. This risk model is only suitable for LKB1-mutant tumors; it performed poorly in LUAD patients with wild-type LKB1.Conclusion: Our findings indicate the potential role of immunity in LKB1-mutant LUAD, providing novel insights into prognosis and guiding effective immunotherapy.</p

Characteristics of patients receiving chemotherapeutic regimens according to RRM1 expression.

<p>*Based on Fisher’s exact test.</p>#<p>Based on Student’s <i>t</i>-test.</p

Tailored pore structure of ZIF-8/chitosan-derived carbonaceous adsorbent by introducing mesoporous silica nanoparticles for superior CO₂ uptake

The shaped hierarchical porous carbon derived from ZIF-8, MSNs (mesoporous silica nanoparticles) and CS (chitosan) has been prepared by in situ assembly and post-annealing process. By tunning carbonization temperatures (from 450 to 650 °C), a series of CS/ZIF-8/MSNs carbonaceous millimetre-sized spheres with honeycomb internal structures and hierarchical pores have been developed for CO2 uptake. CS/ZIF-8/MSNs-525 (the sample treated at 525 °C) exhibited a higher CO2 capacity (1.32 mmol/g, 1 atm) than that of other samples due to its appropriate mesopores (∼11 nm), micropores (∼0.73 nm) and BET surface area (∼459.2 m2g−1). The XRD pattern of CS/ZIF-8/MSNs-525 suggested that MSNs would not affect sample structure, which performed largely maintained ZIF-8 diffraction. The SEM/EDS image of CS/ZIF-8/MSNs-525 showed that MSNs (∼50 nm) has been well decorated onto the surface of ZIF-8 by in situ assembly and carbonization, thus indicating a hierarchical structure formed. In addition, the TG-MS technology has been also utilized to clarify that MSNs assembly would not affect the carbonization process during the formation of CS/ZIF-8/MSNs-525. Moreover, the addition of MSNs facilitated a proper pore structure and higher CO2 capacity of CS/ZIF-8/MSNs-525 compared with that of CS/ZIF-8 carbon spheres (0.9 mmol/g, 1 atm). Particularly, the CS/ZIF-8/MSNs-525 exhibited excellent stability and recyclable performance after CO2 adsorption in 10 adsorption-desorption cycles. This MSNs assembly strategy could induce a simple way for obtaining novel shaped carbonaceous adsorbent for superior CO2 uptake in industrial fields.</p

Strategy to Control the Chromism and Fluorescence Emission of a Perylene Dye in Composite Organogel Phases

Composite organogels based on 1,3,5-tris(4-dodecyloxybenzoylamino)phenylbenzene (DBAPB), a known gelator, and N,N′-di(octadecyl)-perylene-3,4,9,10-tetracarboxylic diimide (C18PTCDI), a nongelator dye, have been achieved, leading to controllable color and emitting color changes. SEM images and XRD patterns revealed that the packing of the DBAPB-based gelator could almost be maintained in the composite gels. The temperature-dependent UV−vis absorption and temperature-dependent fluorescence emission spectra illustrated that the color and emitting color of the composite gels could be controlled by the content of C18PTCDI as well as the temperature in the gel phases. When the content of C18PTCDI was 1 mol %, C18PTCDI could be isolated as unimolecules in the composite gel, which was yellow and gave bright greenish-yellow emission under 365 nm light. For the mixed systems containing 2−10 mol % C18PTCDI, the fresh gels, which were obtained after cooling the hot solutions for a short time, were yellow and produced greenish-yellow emission under 365 nm illumination. However, the corresponding stable composite gels, which were obtained via prolonging the cooling time, were red and emitted weak red emission excited by UV light as a result of the formation of C18PTCDI aggregates. The reversible color and emitting color changes could be realized in the gel phases over a narrow temperature range. Moreover, the excitation energy of DBAPB could be transferred to C18PTCDI in the composite gels, leading to obvious emission quenching of the former

Response to chemotherapy according to RRM1 expression.

<p>*Based on Fisher’s exact test. In patients receiving gemcitabine-based therapy, the DCR of RRM1-negative patients was higher than RRM1-positive patients (<i>P = </i>0.041).</p>#<p>Based on Fisher’s exact test. In patients with RRM1-positive tumors, the DCRs for docetaxel and vinorelbine were higher than for gemcitabine-based therapy (<i>P = </i>0.047 and <i>P = </i>0.047, respectively).</p

Fluorescent Probe for Imaging Hydrogen Polysulfide Dynamics of Macrophage Inflammatory Regulation in Bone Homeostasis

Herein, a fluorescent probe BFO-HPS was developed for imaging the H2Sn dynamics of the macrophage inflammatory regulation in bone homeostasis. The probe indicated advantages including large response scale, rapid response, steady optical performance in various pH conditions, high selectivity, and good selectivity. BFO-HPS showed a dose-dependent fluorescence response at 570 nm for the H2Sn concentration with the linear range from 0.1 to 100 µM, limit of detection of 0.11 µM, and limit of quantitation of 0.10 µM. Based on the low cyto-toxicity, BFO-HPS provided the intracellular monitoring of the exogenous H2Sn level in uninduced RAW264.7 cells. In the receptor activator of nuclear factor-κB ligand (RANKL)-induced and RANKL-induced inhibited groups, BFO-HPS achieved the monitoring of the H2Sn dynamics to indicate the macrophage-involved bone homeostasis. This work was informative for further investigations on macrophage inflammatory regulation in bone homeostasis.</p

Synthesis and Gas Separation Properties of Aromatic Polyimides Containing Noncoplanar Rigid Sites

Four aromatic polyimides were prepared using a conventional two-stage polymerization reaction of diamine monomers containing different noncoplanar rigid sites with commercially available dianhydride 6FDA. All polymers displayed high relative molecular masses, excellent solubilities, and outstanding thermal properties with Tg values higher than 260 °C, which ensured that they were able to be casted as dense films for gas permeation measurement. The comprehensive gas transport properties of four prepared polyimides were higher than conventional aromatic polyimides (e.g., Matrimid 5218 and Kapton), which suggested that the incorporation of the noncoplanar rigid groups into the polymer main chain was beneficial to prevent segment packing as well as to enhance the gas separation properties. The pure CO2 permeability coefficient of the spirobichroman-based polyimide (6FDA-BDA) was 2.9 times as high as that of Matrimid 5218, without sacrificing selectivity. Moreover, the effect of different noncoplanar rigid sites on the gas separation performance of aromatic polyimides was systematically investigated. By measurements of FFV, density, and d-spacing, it has been observed that the incorporation of different noncoplanar rigid sites significantly affected the microstructure, thus affecting the gas transport. For example, because the polyimide containing pendent phenyl groups (6FDA-BDM) had the lowest FFV and the densest segment packing, it exhibited the lowest permeability. Hence, the basic insights to the structure/property relationships of the four polyimides obtained in this work offer a meaningful guide for next-generation gas separation membranes

Basic characteristics of NSCLC patients.

<p>Basic characteristics of NSCLC patients.</p