Synthesis, Characterization, and Thermodynamic Properties of the Rare Earth Coordination Complex [Sm(C₆H₄NO₂)₂·C₉H₆NO]

This article reports the synthesis and thermodynamic properties of a novel rare earth coordination complex, samarium chloride hexahydrate (SmCl₃·6H₂O) with nicotinic acid (C₆H₅NO₂) and 8-hydroxylquinoline (C₉H₇NO), whose composition and structure were characterized by elemental analysis, molar conductance, thermogravimetric analysis (TG–DTG), UV spectroscopy, IR spectroscopy, and X-ray powder diffraction. During the process of coordination, C₆H₅NO₂ was bidentate-coordinated with the rare earth ion (Sm³⁺) through an acidic group that was formed by removing the proton; the hydroxyl oxygen atom and heterocyclic nitrogen atom of C₉H₆NO^– formed a chelate ring with Sm³⁺ for coordination. The X-ray powder diffraction pattern demonstrated that the crystal type of [Sm­(C₆H₄NO₂)₂·C₉H₆NO] is similar to that of C₅H₁₁NO₂, with the cell parameters <i>a</i> = 5.426 nm, <i>b</i> = 22.105 nm, and <i>c</i> = 5.277 nm. At a constant temperature of 298.15 K, the dissolution enthalpies of the reactants and products of the coordination reaction in the optimized calorimetric solvent were determined with an advanced solution–reaction isoperibol microcalorimeter. The standard molar enthalpy change of the coordination reaction was determined to be Δ_r<i>H</i>_m^Θ = (167.49 ± 0.39) kJ·mol^–1. The standard molar enthalpy of formation of the title complex, [Sm­(C₆H₄NO₂)₂·C₉H₆NO], was estimated to be Δ_f<i>H</i>_m^Θ[Sm­(C₆H₄NO₂)₂·C₉H₆NO(s), 298.15 K] = −(1483.4 ± 2.4) kJ·mol^–1, from a combination of the experimental values of enthalpies of dissolution and some other auxiliary thermodynamic data through a designed thermochemical cycle based on a supposed chemical reaction

Table_4_Nanopore long-read RNAseq reveals transcriptional variations in citrus species.xlsx

The number of studies on plant transcriptomes using ONT RNAseq technology is rapidly increasing in recent. It is a powerful method to decipher transcriptomic complexity, particularly alternative splicing (AS) event detection. Citrus plants are the most important widely grown fruit crops. Exploring different AS events in citrus contributes to transcriptome improvement and functional genome study. Here, we performed ONT RNAseq in 9 species (Atalantia buxifolia, Citrus clementina, C. grandis, C. ichangensis, C. reticulata, C. sinensis, Clausena lansium, Fortunella hindsii, and Poncirus trifoliata), accompanied with Illumina sequencing. Non-redundant full-length isoforms were identified between 41,957 and 76,974 per species. Systematic analysis including different types of isoforms, number of isoforms per gene locus, isoform distribution, ORFs and lncRNA prediction and functional annotation were performed mainly focused on novel isoforms, unraveling the capability of novel isoforms detection and characterization. For AS events prediction, A3, RI, and AF were overwhelming types across 9 species. We analyzed isoform similarity and evolutionary relationships in all species. We identified that multiple isoforms derived from orthologous single copy genes among different species were annotated as enzymes, nuclear-related proteins or receptors. Isoforms with extending sequences on 5’, 3’, or both compared with reference genome were filtered out to provide information for transcriptome improvement. Our results provide novel insight into comprehending complex transcriptomes in citrus and valuable information for further investigation on the function of genes with diverse isoforms.</p

DataSheet_1_Nanopore long-read RNAseq reveals transcriptional variations in citrus species.pdf

The number of studies on plant transcriptomes using ONT RNAseq technology is rapidly increasing in recent. It is a powerful method to decipher transcriptomic complexity, particularly alternative splicing (AS) event detection. Citrus plants are the most important widely grown fruit crops. Exploring different AS events in citrus contributes to transcriptome improvement and functional genome study. Here, we performed ONT RNAseq in 9 species (Atalantia buxifolia, Citrus clementina, C. grandis, C. ichangensis, C. reticulata, C. sinensis, Clausena lansium, Fortunella hindsii, and Poncirus trifoliata), accompanied with Illumina sequencing. Non-redundant full-length isoforms were identified between 41,957 and 76,974 per species. Systematic analysis including different types of isoforms, number of isoforms per gene locus, isoform distribution, ORFs and lncRNA prediction and functional annotation were performed mainly focused on novel isoforms, unraveling the capability of novel isoforms detection and characterization. For AS events prediction, A3, RI, and AF were overwhelming types across 9 species. We analyzed isoform similarity and evolutionary relationships in all species. We identified that multiple isoforms derived from orthologous single copy genes among different species were annotated as enzymes, nuclear-related proteins or receptors. Isoforms with extending sequences on 5’, 3’, or both compared with reference genome were filtered out to provide information for transcriptome improvement. Our results provide novel insight into comprehending complex transcriptomes in citrus and valuable information for further investigation on the function of genes with diverse isoforms.</p

Table_1_Nanopore long-read RNAseq reveals transcriptional variations in citrus species.xlsx

The number of studies on plant transcriptomes using ONT RNAseq technology is rapidly increasing in recent. It is a powerful method to decipher transcriptomic complexity, particularly alternative splicing (AS) event detection. Citrus plants are the most important widely grown fruit crops. Exploring different AS events in citrus contributes to transcriptome improvement and functional genome study. Here, we performed ONT RNAseq in 9 species (Atalantia buxifolia, Citrus clementina, C. grandis, C. ichangensis, C. reticulata, C. sinensis, Clausena lansium, Fortunella hindsii, and Poncirus trifoliata), accompanied with Illumina sequencing. Non-redundant full-length isoforms were identified between 41,957 and 76,974 per species. Systematic analysis including different types of isoforms, number of isoforms per gene locus, isoform distribution, ORFs and lncRNA prediction and functional annotation were performed mainly focused on novel isoforms, unraveling the capability of novel isoforms detection and characterization. For AS events prediction, A3, RI, and AF were overwhelming types across 9 species. We analyzed isoform similarity and evolutionary relationships in all species. We identified that multiple isoforms derived from orthologous single copy genes among different species were annotated as enzymes, nuclear-related proteins or receptors. Isoforms with extending sequences on 5’, 3’, or both compared with reference genome were filtered out to provide information for transcriptome improvement. Our results provide novel insight into comprehending complex transcriptomes in citrus and valuable information for further investigation on the function of genes with diverse isoforms.</p

Table_3_Nanopore long-read RNAseq reveals transcriptional variations in citrus species.xlsx

The number of studies on plant transcriptomes using ONT RNAseq technology is rapidly increasing in recent. It is a powerful method to decipher transcriptomic complexity, particularly alternative splicing (AS) event detection. Citrus plants are the most important widely grown fruit crops. Exploring different AS events in citrus contributes to transcriptome improvement and functional genome study. Here, we performed ONT RNAseq in 9 species (Atalantia buxifolia, Citrus clementina, C. grandis, C. ichangensis, C. reticulata, C. sinensis, Clausena lansium, Fortunella hindsii, and Poncirus trifoliata), accompanied with Illumina sequencing. Non-redundant full-length isoforms were identified between 41,957 and 76,974 per species. Systematic analysis including different types of isoforms, number of isoforms per gene locus, isoform distribution, ORFs and lncRNA prediction and functional annotation were performed mainly focused on novel isoforms, unraveling the capability of novel isoforms detection and characterization. For AS events prediction, A3, RI, and AF were overwhelming types across 9 species. We analyzed isoform similarity and evolutionary relationships in all species. We identified that multiple isoforms derived from orthologous single copy genes among different species were annotated as enzymes, nuclear-related proteins or receptors. Isoforms with extending sequences on 5’, 3’, or both compared with reference genome were filtered out to provide information for transcriptome improvement. Our results provide novel insight into comprehending complex transcriptomes in citrus and valuable information for further investigation on the function of genes with diverse isoforms.</p

Table_2_Nanopore long-read RNAseq reveals transcriptional variations in citrus species.xlsx

The number of studies on plant transcriptomes using ONT RNAseq technology is rapidly increasing in recent. It is a powerful method to decipher transcriptomic complexity, particularly alternative splicing (AS) event detection. Citrus plants are the most important widely grown fruit crops. Exploring different AS events in citrus contributes to transcriptome improvement and functional genome study. Here, we performed ONT RNAseq in 9 species (Atalantia buxifolia, Citrus clementina, C. grandis, C. ichangensis, C. reticulata, C. sinensis, Clausena lansium, Fortunella hindsii, and Poncirus trifoliata), accompanied with Illumina sequencing. Non-redundant full-length isoforms were identified between 41,957 and 76,974 per species. Systematic analysis including different types of isoforms, number of isoforms per gene locus, isoform distribution, ORFs and lncRNA prediction and functional annotation were performed mainly focused on novel isoforms, unraveling the capability of novel isoforms detection and characterization. For AS events prediction, A3, RI, and AF were overwhelming types across 9 species. We analyzed isoform similarity and evolutionary relationships in all species. We identified that multiple isoforms derived from orthologous single copy genes among different species were annotated as enzymes, nuclear-related proteins or receptors. Isoforms with extending sequences on 5’, 3’, or both compared with reference genome were filtered out to provide information for transcriptome improvement. Our results provide novel insight into comprehending complex transcriptomes in citrus and valuable information for further investigation on the function of genes with diverse isoforms.</p

Table_5_Nanopore long-read RNAseq reveals transcriptional variations in citrus species.xlsx

The number of studies on plant transcriptomes using ONT RNAseq technology is rapidly increasing in recent. It is a powerful method to decipher transcriptomic complexity, particularly alternative splicing (AS) event detection. Citrus plants are the most important widely grown fruit crops. Exploring different AS events in citrus contributes to transcriptome improvement and functional genome study. Here, we performed ONT RNAseq in 9 species (Atalantia buxifolia, Citrus clementina, C. grandis, C. ichangensis, C. reticulata, C. sinensis, Clausena lansium, Fortunella hindsii, and Poncirus trifoliata), accompanied with Illumina sequencing. Non-redundant full-length isoforms were identified between 41,957 and 76,974 per species. Systematic analysis including different types of isoforms, number of isoforms per gene locus, isoform distribution, ORFs and lncRNA prediction and functional annotation were performed mainly focused on novel isoforms, unraveling the capability of novel isoforms detection and characterization. For AS events prediction, A3, RI, and AF were overwhelming types across 9 species. We analyzed isoform similarity and evolutionary relationships in all species. We identified that multiple isoforms derived from orthologous single copy genes among different species were annotated as enzymes, nuclear-related proteins or receptors. Isoforms with extending sequences on 5’, 3’, or both compared with reference genome were filtered out to provide information for transcriptome improvement. Our results provide novel insight into comprehending complex transcriptomes in citrus and valuable information for further investigation on the function of genes with diverse isoforms.</p

Metal–Organic Rotaxane Frameworks Assembly by Cucurbit[6]uril-Based Pseudorotaxanes and Mixed Ligands

Four novel high-dimensional metal–organic rotaxane frameworks (MORFs) were successfully fabricated by cucurbit[6]­uril-based pseudorotaxanes ([PR43]) and rigid carboxylate ligands by adjusting the synthesis temperature and the length of ligands. Compound <b>1</b> represents a three periodic 2-fold interpenetrating dia network based on a binuclear {Cd₂Cl₄O₄} second building unit (SBU). <b>2</b> and <b>3</b> are isostructural pcu networks constructed by binuclear and trinuclear cadmium clusters, respectively, while compound <b>4</b> is a two-dimensional layered network with a large grid 44.3 × 19.8 Ų. Furthermore, their fluorescent properties in different solvents are investigated

Metal–Organic Rotaxane Frameworks Assembly by Cucurbit[6]uril-Based Pseudorotaxanes and Mixed Ligands

Four novel high-dimensional metal–organic rotaxane frameworks (MORFs) were successfully fabricated by cucurbit[6]­uril-based pseudorotaxanes ([PR43]) and rigid carboxylate ligands by adjusting the synthesis temperature and the length of ligands. Compound <b>1</b> represents a three periodic 2-fold interpenetrating dia network based on a binuclear {Cd₂Cl₄O₄} second building unit (SBU). <b>2</b> and <b>3</b> are isostructural pcu networks constructed by binuclear and trinuclear cadmium clusters, respectively, while compound <b>4</b> is a two-dimensional layered network with a large grid 44.3 × 19.8 Ų. Furthermore, their fluorescent properties in different solvents are investigated