Additional file 1 of miR-107 is involved in the regulation of NEDD9-mediated invasion and metastasis in breast cancer

Additional file 1: Supplemental Figure S1. MiR-107 is involved in promoting the migration, invasion and proliferation of MDA-MB-231 cells. (a) The scratch test was used to assess the migration ability of stably transfected lines with miR-107 overexpression and miR-107 silencing as well as of empty vector-transfected cells. Scale bar = 100 μm. (b) Transwell assays were selected to assess the migration ability of cells with miR-107 overexpression and miR-107 silencing as well as of empty vector-transfected MCF-7 cells. Scale bar = 100 μm. (c) The colony formation assay was used to evaluate the proliferation ability of stably transfected cells with miR-107 overexpression and miR-107 silencing as well as cells transfected with empty vector. ***p < 0.001, **p < 0.01, *p < 0.05. (d) Statistical analysis of wound closure ability (n = 6). (e) Statistical analysis of the migration ability of cells (n = 6). (f) Statistical analysis of the proliferation ability of cells (n = 6)

Additional file 4 of miR-107 is involved in the regulation of NEDD9-mediated invasion and metastasis in breast cancer

Additional file 4: Supplemental Table S2. Description of the parameters and values shown in the results delivered by TargetScan, miRanda and Diana Tools

Mechanism of Synergistic Effect on Electron Transfer over Co–Ce/MCM-48 during Ozonation of Pharmaceuticals in Water

The same amount of metal was deposited on the surface of three-dimensional mesoporous MCM-48 by a facile impregnation–calcination method for catalytic ozonation of pharmaceutical and personal-care products in the liquid phase. At 120 min reaction time, Co/MCM-48 and Ce/MCM-48 showed 46.6 and 63.8% mineralization for clofibric acid (CA) degradation, respectively. Less than 33% mineralization was achieved with Co/MCM-48 and Ce/MCM-48 during sulfamethazine (SMZ) ozonation. In the presence of monometallic oxides modified MCM-48 catalysts, total organic carbon (TOC) removal of diclofenac sodium (DCF) was around 80%. The composite Co–Ce/MCM-48 catalyst exhibited significantly higher activity in terms of TOC removal of CA (83.6%), SMZ (51.7%) and DCF (86.8%). Co–Ce/MCM-48 inhibited efficiently the accumulation of small molecular carboxyl acids during ozonation. A detailed research was conducted to detect the nature of material structure and mechanism of catalytic ozonation by using a series of characterizations. The main reaction pathway of CA was determined by the analysis of liquid chromatography-mass spectrometry, in line with the results of frontier electron density calculations that reactive oxygen species (ROSs) were easy to attack negative regions of pharmaceuticals. The Si–O–Si, Co···HO–Si–O–Si–OH···Ce, and O3···Co–HO–Si–O–Si–OH···Ce–OH···O3 basic units in catalysts were constructed to detect the orbit-energy-level difference. The results revealed that a synergistic effect existed at the interface between cobalt and cerium oxides over MCM-48, which facilitated the ROSs sequence in solution with ozone. Therefore, the multivalence redox coupling of Ce4+/Ce3+ and Co3+/Co2+ along with electron transfer played an important role in catalytic ozonation process

Additional file 5 of miR-107 is involved in the regulation of NEDD9-mediated invasion and metastasis in breast cancer

Additional file 5

Additional file 2 of miR-107 is involved in the regulation of NEDD9-mediated invasion and metastasis in breast cancer

Additional file 2: Supplemental Figure S2. miR-107 contributes to the inhibition of breast cancer development in an orthotopic breast cancer model. (a) Stably transfected MDA-MB-231 cells overexpressing miR-107 were injected into subcutaneous mammary fat pads of nude mice. Diagram of subcutaneous tumour formation in nude mice. (b) Body weight curve. (c, d) Tumour volume of xenograft tumours in different groups. NC, control group; miR-107-OE, miR-107 overexpression group; NC-OE, miR-107 empty group. n = 5; **p < 0.01

Additional file 3 of miR-107 is involved in the regulation of NEDD9-mediated invasion and metastasis in breast cancer

Additional file 3: Supplemental Table S1. The database for miRNA sequence-based prediction