The influence of the subsidy coefficient on the manufacturer’s profit.

The influence of the subsidy coefficient on the manufacturer’s profit.</p

The influence of the subsidy coefficient on the wholesale price.

The influence of the subsidy coefficient on the wholesale price.</p

The influence of the subsidy coefficient on the assembly rate.

The influence of the subsidy coefficient on the assembly rate.</p

The influence of the subsidy coefficient on the assembler’s profit.

The influence of the subsidy coefficient on the assembler’s profit.</p

The influence of the subsidy coefficient on the retail price.

The influence of the subsidy coefficient on the retail price.</p

Notations of parameters and variables.

Notations of parameters and variables.</p

Data file.

(XLSX)</p

S1 Raw images -

(PDF)</p

Pyroptosis was dependent on caspase-1 in ZIKV-infected human and murine macrophages.

Cell lysates were prepared at various time points p.i. from ZIKV-infected THP-1 (A) and RAW264.7 (B) cells which were untreated or pre-treated with VX765, a caspase-1 inhibitor, at 20μM. The lysates were analysed by western blot analyses with an anti-cleaved caspase-1 antibody. Morphological changes were observed on ZIKV-infected THP-1 (C) and RAW264.7. Arrows, swollen or ruptured cells. (D) cells, which were untreated or pre-treated with VX765, under a light microscope (Magnification x200). (E & F) Cell viability of THP-1 and RAW264.7, pre-treated with or without VX765 and followed by ZIKV infection (0.1 MOI), was measured by a CCK8 assay. (G & H) Culture medium was sampled at various time points p.i. from ZIKV-infected THP-1 and RAW264.7 cells, which were untreated or pre-treated with VX765, for measurement of secreted IL-1β by ELISA. The experiments were performed in triplicates and the data were shown as mean+SD and analyzed by unpaired Students t-test. *, P<0.05; **, P<0.01; ***, P<0.001. ns, no significance.</p

ZIKV infection induced apoptosis in macrophages.

PMA-differentiated THP-1 cells were infected with ZIKV and observed under a light microscope at 12 and 24 hrs p.i. for the cells showing apoptotic blebing (arrow) (A). The cells were treated with 10 μM of apoptosis activator 2, a caspase3 activator, for 2 hrs as a positive control. A TUNEL assay was applied to ZIKV-infected THP-1 cells at 12 and 24 hrs p.i. and the TUNEL positive cells were shown (arrow). The cells were treated with 20 U/mL of DNase I for 10 min as a positive control (B).</p