Remodelling of human atrial K+ currents but not ion channel expression by chronic β-blockade

Chronic β-adrenoceptor antagonist (β-blocker) treatment in patients is associated with a potentially anti-arrhythmic prolongation of the atrial action potential duration (APD), which may involve remodelling of repolarising K+ currents. The aim of this study was to investigate the effects of chronic β-blockade on transient outward, sustained and inward rectifier K+ currents (ITO, IKSUS and IK1) in human atrial myocytes and on the expression of underlying ion channel subunits. Ion currents were recorded from human right atrial isolated myocytes using the whole-cell-patch clamp technique. Tissue mRNA and protein levels were measured using real time RT-PCR and Western blotting. Chronic β-blockade was associated with a 41% reduction in ITO density: 9.3 ± 0.8 (30 myocytes, 15 patients) vs 15.7 ± 1.1 pA/pF (32, 14), p < 0.05; without affecting its voltage-, time- or rate dependence. IK1 was reduced by 34% at −120 mV (p < 0.05). Neither IKSUS, nor its increase by acute β-stimulation with isoprenaline, was affected by chronic β-blockade. Mathematical modelling suggested that the combination of ITO- and IK1-decrease could result in a 28% increase in APD90. Chronic β-blockade did not alter mRNA or protein expression of the ITO pore-forming subunit, Kv4.3, or mRNA expression of the accessory subunits KChIP2, KChAP, Kvβ1, Kvβ2 or frequenin. There was no reduction in mRNA expression of Kir2.1 or TWIK to account for the reduction in IK1. A reduction in atrial ITO and IK1 associated with chronic β-blocker treatment in patients may contribute to the associated action potential prolongation, and this cannot be explained by a reduction in expression of associated ion channel subunits

Form factors and photoproduction amplitudes

We examine the use of phenomenological form factors in tree level amplitudes for meson photoproduction. Two common recipes are shown to be fundamentally incorrect. An alternate form consistent with gauge invariance and crossing symmetry is proposed.Comment: To be published in PR

Gauge-invariant tree-level photoproduction amplitudes with form factors

We show how the gauge-invariance formulation given by Haberzettl is implemented in practice for photoproduction amplitudes at the tree level with form factors describing composite nucleons. We demonstrate that, in contrast to Ohta's gauge-invariance prescription, this formalism allows electric current contributions to be multiplied by a form factor, i.e., it does not require that they be treated like bare currents. While different in detail, this nevertheless lends support to previous ad hoc approaches which multiply the Born amplitudes by an overall form factor. Numerical results for kaon photoproduction off the nucleon are given. They show that the gauge procedure by Haberzettl leads to much improved $\chi^2$ values as compared to Ohta's prescription.Comment: 5 pages, RevTeX, two eps figure

Extraction of the D13(1520) photon-decay couplings from pion- and eta-photoproduction data

We compare results for the D13(1520) photon-decay amplitudes determined in analyses of eta- and pion-photoproduction data. The ratio of helicity amplitudes (A_3/2 / A_1/2), determined from eta-photoproduction data, is quite different from that determined in previous analyses of pion-photoproduction data. We consider how strongly the existing pion-photoproduction data constrain both this ratio and the individual photon-decay amplitudes.Comment: 7 pages, 2 figure

Coherent Pair State of Pion in Constituent Quark Model

A coherent state of pions is introduced to the nonrelativistic quark model. The coherent pair approximation is employed for the pion field in order to maintain the spin-isospin symmetry. In this approximation the pion is localized in the momentum space, and the vertex form factor in the pion-quark interaction is derived from this localization. The nucleon masses and wave functions are calculated using this model, and our results are compared to those of the quark model with the one pion exchange potential. Similar result is obtained for the mass spectrum, but there exists a clear difference in the internal structure of nucleon resonances.Comment: 17 pages, 2 figures, revtex, submitted to Phys. Rev.

Low-energy Pion-nucleon Scattering

This paper contains the results of an analysis of recent low-energy pion-nucleon scattering experiments. Obtained are phase shifts, the pion-nucleon coupling constant and an estimate of the Sigma term.Comment: 30 pages, 11 figures, LaTe

Virtual-pion and two-photon production in pp scattering

Two-photon production in pp scattering is proposed as a means of studying virtual-pion emission. Such a process is complementary to real-pion emission in pp scattering. The virtual-pion signal is embedded in a background of double-photon bremsstrahlung. We have developed a model to describe this background process and show that in certain parts of phase space the virtual-pion signal gives significant contribution. In addition, through interference with the two-photon bremsstrahlung background, one can determine the relative phase of the virtual-pion process

The energies and residues of the nucleon resonances N(1535) and N(1650)

We extract pole positions for the N(1535) and N(1650) resonances using two different models. The positions are determined from fits to different subsets of the existing $\pi N\to\pi N$, $\pi N\to\eta N$ and $\gamma p\to\eta p$ data and found to be 1515(10)--i85(15)MeV and 1660(10)--i65(10)MeV, when the data is described in terms of two poles. Sensitivity to the choice of fitted data is explored. The corresponding $\pi \pi$ and $\eta \eta$ residues of these poles are also extracted.Comment: 9 page

Problems with Extraction of the Nucleon to Delta(1232) Photonic Amplitudes

We investigate the model dependence and the importance of choice of database in extracting the {\it physical} nucleon-Delta(1232) electromagnetic transition amplitudes, of interest to QCD and baryon structure, from the pion photoproduction observables. The model dependence is found to be much smaller than the range of values obtained when different datasets are fitted. In addition, some inconsistencies in the current database are discovered, and their affect on the extracted transition amplitudes is discussed.Comment: Revtex, 2 figs., submitted to PR

Baryon Octet to Decuplet Electromagnetic Transitions

The electromagnetic transition moments of the $SU(3)$-flavor baryon octet to decuplet are examined within a lattice simulation of quenched QCD. The magnetic transition moment for the $N \; \gamma \to \Delta$ channel is found to be in agreement with recent experimental analyses. The lattice results indicate $\mu_{p \Delta} / \mu_p = 0.88(15)$. In terms of the Particle Data Group convention, $f_{M1} = 0.231(41)$ GeV${}^{-1/2}$ for $p \; \gamma \to \Delta^+$ transitions. Lattice predictions for the hyperon $M1$ transition moments agree with those of a simple quark model. However the manner in which the quarks contribute to the transition moments in the lattice simulation is different from that anticipated by quark model calculations. The scalar quadrupole form factor exhibits a behavior consistent with previous multipole analyses. The $E2/M1$ multipole transition moment ratios are also determined. The lattice results suggest $R_{EM} \equiv -{\cal G}_{E2}/{\cal G}_{M1} = +3\pm 8$ \% for $p \; \gamma \to \Delta^+$ transitions. Of particular interest are significant nonvanishing signals for the $E2/M1$ ratio in $\Xi^-$ and $\Sigma^-$ electromagnetic transitions.Comment: PostScript file, 37 pages including figures. U. MD PP #93-085, U. KY PP #UK/92-09, TRIUMF PP #TRI-PP-92-12