Characterization of monoclonal and polyclonal antibodies to bovine enteric coronavirus: Establishment of an efficient ELISA for antigen detection in feces

Monoclonal antibodies to bovine enteric coronavirus (BEC) were produced. Additionally, polyclonal antibodies were made in rabbits and guinea pigs and extracted from the yolk of immunized hens. The antibodies were characterized by neutralization test, hemagglutination inhibition test, enzyme-linked immunosorbent assay (ELISA) and immunoblotting. Neutralizing antibody titers of polyclonal antisera ranged from 1:1280 to 1:40 000. Only one out of 908 hybridoma colonies tested secreted antibodies with neutralizing activity. By ELISA, polyclonal sera exhibited high background reactions that could be significantly reduced by treatment with kaolin in the case of rabbit sera. Attempts to establish an ELISA for BEC antigen detection based on polyclonal sera failed due to low sensitivity and specificity. Optimal results were achieved when a mixture of two monoclonal antibodies was coated onto microplates for antigen capture, while rabbit hyperimmune serum served as detecting antibodies in an indirect assay. The combination of the two monoclonal antibodies did not increase sensitivity synergistically, but in a compensatory fashion, probably because of epitope differences between BEC field strain

Kinematical and mechanical aspects of wafer slicing

Some recently achieved results concerning the technological fundamentals of slurry sawing are presented. The specific material removal process and the related kinematic and geometric contact conditions between workpiece and saw blade are described. The result of a functional description of the slurry sawing process is presented, expressing the main process criteria, such as infeed per stroke, specific removal rate, specific tool wear, and vertical stroke intensity, in terms of the dominating process parameters, such as stroke length, width of workpiece, stroke frequency, specific cutting force and slurry specification

The "Ridge" in Proton-Proton Scattering at 7 TeV

One of the most important experimental results for proton-proton scattering at the LHC is the observation of a so-called "ridge" structure in the two particle correlation function versus the pseudorapidity difference $\Delta\eta$ and the azimuthal angle difference $\Delta\phi$. One finds a strong correlation around $\Delta\phi=0$, extended over many units in $\Delta\eta$. We show that a hydrodynamical expansion based on flux tube initial conditions leads in a natural way to the observed structure. To get this result, we have to perform an event-by-event calculation, because the effect is due to statistical fluctuations of the initial conditions, together with a subsequent collective expansion. This is a strong point in favour of a fluid-like behavior even in $pp$ scattering, where we have to deal with length scales of the order of 0.1 fm.Comment: 5 pages, 4 figure