The Nei's genetic distance between species pairs in <i>Physalis</i>.

<p>The Nei's genetic distance between species pairs in <i>Physalis</i>.</p

Number of polymorphic markers and alleles amplified from 38 <i>Physalis</i> accessions and 25 <i>P. philadelphica</i> accessions.

<p>The accessions with ambiguous species information were excluded from <i>P. philadelphica</i> cluster for calculation.</p

Distribution of Nei's genetic distance values.

<p>The genetic distance was obtained from pair wise comparisons of 38 accessions of <i>Physalis</i> and 23 accessions of <i>P</i>. <i>philadelphica</i> with molecular marker data using the software NTSYSpc 2.11a.</p

Information for 38 accessions of the genus <i>Physalis</i> and two tomato lines used in this study.

<p>The information for all except the tomato line OH88119 were obtained from the website of Northeast Regional PI Station at Geneva, New York, USA (<a href="http://www.ars-grin.gov" target="_blank">http://www.ars-grin.gov</a>).</p

Dendrogram of 38 <i>Physalis</i> accessions based on all marker data (A) and <i>P. peruviana</i> SSR marker data (B).

<p>The dendrogram was generated from Nei’s genetic distance matrix by UPGMA in NTSYSpc 2.11a. Tomato lines OH88119 and PI435238 were used as out-group controls.</p

Population structure of 38 <i>Physalis</i> accessions and two tomato lines using STRUCTURE software and 122 markers.

<p>The coefficients of estimated ancestry per accession in each cluster were represented by an individual bar, where each color refers to a distinct cluster. The name of the accession is below the bar.</p

PCR successes and number of alleles amplified in 38 accessions of the genus <i>Physalis</i> and two tomato lines.

<p>PCR successes and number of alleles amplified in 38 accessions of the genus <i>Physalis</i> and two tomato lines.</p

qRT-PCR analysis of 10 differentially expressed TDFs in tomato.

<p>PT: PI 114490 inoculated with T3. PM: PI 114490 mock-inoculated with the sterile solution containing 10 mM MgSO₄·7H₂O and 0.025% (v/v) Silwet L77. OT: OH 88119 inoculated with T3. OM: OH 88119 mock-inoculated with the sterile solution containing 10 mM MgSO₄·7H₂O and 0.025% (v/v) Silwet L77. Gene expression was determined relative to <i>EF1-α</i> transcript levels in the same samples. The data are means SD of three experimental replicates. The asterisk above the bars indicates statistically significant differences between the infected samples and corresponding mock treatment.</p

Images of cDNA-AFLP and Semi-quantitative RT-PCR analysis of 19 representative TDFs in leaves of tomato lines PI 114490 and OH 88119 at various time-points after inoculation of bacterial spot race T3 strain <i>Xv829</i>.

<p>PT: PI 114490 inoculated with T3. PM: PI 114490 mock-inoculated with the sterile solution containing 10 mM MgSO₄·7H₂O and 0.025% (v/v) Silwet L77. OT: OH 88119 inoculated with T3. OM: OH 88119 mock-inoculated with the sterile solution containing 10 mM MgSO₄·7H₂O and 0.025% (v/v) Silwet L77. The EF1-α was used as a constitutive control for the RT-PCR.</p

Enumerating main types of differentially expressed fragment during cDNA-AFLP analysis.

<p>PT: PI 114490 inoculated with T3. PM: PI 114490 mock-inoculated with the sterile solution containing 10 mM MgSO₄·7H₂O and 0.025% (v/v) Silwet L77. OT: OH 88119 inoculated with T3. OM: OH 88119 mock-inoculated with the sterile solution containing 10 mM MgSO₄·7H₂O and 0.025% (v/v) Silwet L77. A: Common persistent up-regulated TDF (indicated by an arrow) in both tomato lines. B: Common persistent down-regulated TDF in both tomato lines. C: Specific persistent up-regulated TDF in PI 114490. D: Specific persistent down-regulated TDF in PI 114490. E: Constitutive expressed only in PI 114490. F: Constitutive expressed only in OH 88119. G: Length polymorphism fragment between two tomato lines.</p